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EC number: 413-860-0 | CAS number: 1187-74-2 ACETONYLBERNSTEINSÄUREESTER; ASE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant study conducted to Internation test guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Diethyl 4-oxopentane-1,2-dicarboxylate
- EC Number:
- 413-860-0
- EC Name:
- Diethyl 4-oxopentane-1,2-dicarboxylate
- Cas Number:
- 1187-74-2
- Molecular formula:
- C11H18O5
- IUPAC Name:
- 1,4-diethyl 2-(2-oxopropyl)butanedioate
- Details on test material:
- - Name of test material (as cited in study report): CA787A (intermediate of CGA163935)
- Physical state: Liquid
- Analytical purity: 98.9%
- Purity test date: 1996-06-03
- Lot/batch No.: SKCA001
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature, protected from light
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control) and 100 mg/L
- Sampling method: Direct sampliing of fresh media (study initiation) and, at study end, direct sampling of a composite of the 3 replicates after centrifugation to remove algae. Samples taken from mid-point of test vessel
- Sample storage conditions before analysis: Analysis undertaken on the day of sampling
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution with test medium
- Eluate: OECD medium
- Differential loading: No
- Controls: Yes - OECD medium alone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None observed
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapita
- Strain:
- Source (laboratory, culture collection): Laboratory culture originally sourced from Plant Physiologic Institute Gottingen Germany
- Age of inoculum (at test initiation): 5 days
- Method of cultivation: 250 mL glass flasks containing 100 mL OECD medium, shaking at 90 rpm, constant illumination at 6026-8070 Lux, at 24.3-24.9 degrees C
ACCLIMATION
- Acclimation period: 5 days
- Culturing media and conditions (same as test or not): Same as test conditions
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 24.3-24.8 degrees c
- pH:
- Control: 8.02 at test start, 9.81 at test termination
Test: 7.96 at test start, 9.34 at test termination - Dissolved oxygen:
- Not reported
- Salinity:
- Not relevant for fresh water tests
- Nominal and measured concentrations:
- Nominal: 0 mg/L (control) and 100 mg/L (limit test)
Measured at test start: <5.345 mg/L (control) and 102 mg/L(limit test)
Measured at test termination: <4.746 mg/L (control) and 49 mg/L (Limit test) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed with stainless steel caps
- Material, size, headspace, fill volume: Glass 250 mL flasks, filled with 100 mL medium
- Aeration: none
- Initial cells density: 1E4 cells/mL
- Control end cells density: 137.8E4 cells/mL
- No. of organisms per vessel: 1E6 cells
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Sterile deionised water
- Total organic carbon: Not reported
- Particulate matter: Not reported
- Metals: Meets ASTM E729-88
- Pesticides: Meets ASTM E729-88
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: 5.3
- Conductivity: Not repoted
- Culture medium different from test medium: No
- Intervals of water quality measurement: Annual
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: Yes
- Photoperiod: Continuous
- Light intensity and quality: 6241-7747 lux, cool white fluorescent lamps
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None (study conducted as a limit test)
- Justification for using less concentrations than requested by guideline: Expected lack of toxicity at limit concentration
- Range finding study
- Test concentrations: 0.01 - 100 mg/L
- Results used to determine the conditions for the definitive study: Lack oif growth inhibition at 100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 75.26 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 75.26 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 75.26 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 75.26 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: Bloated appearance
- Colour differences:
- Any stimulation of growth found in any treatment: Yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance tested does not inhibit the growth of aquatic algae
- Executive summary:
The substance tested does not inhibit the growth of aquatic algae
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