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EC number: 932-349-8 | CAS number: 8006-64-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Crude sulfate turpentine is a UVCB substance that contains the same main constituents as gum turpentine oil. Therefore, data on crude sulfate turpentine can be extrapolated to gum turpentine oil. See read-across justification document in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Post exposure observation period:
- None
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 17.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Water Accommodated Fractions (WAFs)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 16.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Water Accommodated Fractions (WAFs)
- Basis for effect:
- biomass
- Remarks:
- (yield)
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Water Accommodated Fractions (WAFs)
- Basis for effect:
- other: growth rate and biomass (yield)
- Details on results:
- - Exponential growth in the control: yes, the cell concentration in the control cultures increased by a factor of 230 in 72 h.
- Effect concentrations exceeding solubility of substance in test medium: the loading rates at which the water-accommodated fractions were prepared exceeded the solubility of the test substance. - Reported statistics and error estimates:
- The results of the Alga Test were analysed statistically with reference to the specific growth rate [µ] and biomass (yield).
Normality of the data was tested using a Kolmogorov-Smirnov-test (α = 0.05).
Homogeneity of variances was tested by Bartlett`s test (α = 0.05).
For all measured end values, statistical differences among treatment groups were determined using a one-way analysis of variance (ANOVA).
Two-sided Dunnet's procedure for multiple comparison was used to determine, which concentration of the test item differed significantly from the control (p = 0.05).
The results of the two-sided Dunnet's test (p = 0.05) were used to determine the NOELR (No Observable Effect Loading Rate) of the specific growth rate [µ] and of the biomass (yield).
The NOELR (No Observable Effect Loading Rate) is defined as the highest test loading rate, where inhibition of the specific growth rate [µ] and of the biomass was statistically not significantly different when compared to the control. - Validity criteria fulfilled:
- yes
- Conclusions:
- A 72h EL50 of 17.1 mg/L and NOELr of 10 mg/L have been determined for the effect of the test substance on growth rate of Desmodesmus subspicatus.
- Executive summary:
An algal toxicity study was performed in accordance with OECD guideline 201 and GLP. Algal cells (Desmodesmus subspicatus) were exposed to Water Accommodated Fractions (WAFs) of the test item, over a range of nominal loading values of 5, 10, 25, 50 and 100 mg/L. The inhibition of growth in relation to control cultures was determined over a test period of 72 h, and thus several algal generations, in closed test vessels.
The EL50 and NOELr at 72 h for the test item to algae (Desmodesmus subspicatus) based on the nominal loading rates were determined to be 17.1 and 10 mg/L, respectively.
Reference
Table 1. Test results – average specific growth rates (µ) over the test period 0-72 hours
Loading rate [mg/L] |
Cells / mL |
Average Specific growth rate [µ] |
Average Specific growth rate [µ] Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Average Specific growth rate [%] |
Inhibition of Average Specific growth rate Mean value [%] |
Control 1 |
576000 |
1.81 |
1.81 |
0.00 |
0.15 |
|
|
Control 2 |
568000 |
1.81 |
|||||
Control 3 |
576000 |
1.81 |
|||||
5.0 |
584000 |
1.82 |
1.81 |
0.01 |
0.45 |
-0.34 |
-0.08 |
5.0 |
560000 |
1.80 |
0.43 |
||||
5.0 |
584000 |
1.82 |
-0.34 |
||||
10 |
568000 |
1.81 |
1.80 |
0.01 |
0.68 |
0.17 |
0.79 |
10 |
528000 |
1.78 |
1.52 |
||||
10 |
552000 |
1.80 |
0.70 |
||||
25 |
5000 |
0.23 |
0.15 |
0.09 |
57.1 |
87.3 |
91.8 |
25 |
3000 |
0.06 |
96.7 |
||||
25 |
4000 |
0.16 |
91.4 |
||||
50 |
4500 |
0.20 |
0.10 |
0.10 |
95.7 |
89.2 |
94.3 |
50 |
2500 |
0.00 |
100 |
||||
50 |
3500 |
0.11 |
93.8 |
||||
100 |
3000 |
0.06 |
0.09 |
0.06 |
59.7 |
96.7 |
94.9 |
100 |
3000 |
0.06 |
96.7 |
||||
100 |
4000 |
0.16 |
91.4 |
Remark: A negative inhibition value means an enhancement of the parameter.
Table 2. Test results – biomass (yield) over the whole test period 0-72 hours
Loading rate [mg/L] |
Cells/mL |
Yield Cells/mL |
Yield Cells/mL Mean value |
Standard deviation |
Coefficient of variation Vc [%] |
Inhibition of Yield [%] |
Inhibition of Yield [%] Average |
Control 1 |
576000 |
573500 |
570833 |
4619 |
0.81 |
|
|
Control 2 |
568000 |
565500 |
|||||
Control 3 |
576000 |
573500 |
|||||
5.0 |
584000 |
581500 |
573500 |
13856 |
2.42 |
-1.87 |
-0.47 |
5.0 |
560000 |
557500 |
2.34 |
||||
5.0 |
584000 |
581500 |
-1.87 |
||||
10 |
568000 |
565500 |
546833 |
20133 |
3.68 |
0.93 |
4.20 |
10 |
528000 |
525500 |
7.94 |
||||
10 |
552000 |
549500 |
3.74 |
||||
25 |
5000 |
2500 |
1500 |
1000 |
66.7 |
99.6 |
99.7 |
25 |
3000 |
500 |
99.9 |
||||
25 |
4000 |
1500 |
99.7 |
||||
50 |
4500 |
2000 |
1000 |
1000 |
100 |
99.7 |
99.8 |
50 |
2500 |
0 |
100 |
||||
50 |
3500 |
1000 |
99.8 |
||||
100 |
3000 |
500 |
833 |
577 |
69.3 |
99.9 |
99.9 |
100 |
3000 |
500 |
99.9 |
||||
100 |
4000 |
1500 |
99.7 |
Remark: A negative inhibition value means an enhancement of the parameter.
Table 3. Results of TOC analysis of the test media
Time [h] |
Control |
5.0 mg/L |
25 mg/L |
100 mg/L |
0 |
0.3 |
0.3 |
1.7 |
4.7 |
72 |
≤0.3 |
0.1 |
1.2 |
3.8 |
Table 4. Results of GC-MS analysis of test media
Vessel |
Time [h] |
a-Terpineol |
a-Pinene |
b-Pinene |
3-Carene |
Limonene |
Total [µg/L] |
Control |
0 |
0.214** |
< 0.367* |
0.227** |
< 0.151* |
0.212** |
< 1.17 |
Control |
72 |
0.214** |
< 0.367* |
0.227** |
< 0.151* |
0.212** |
< 1.17 |
5.0 mg/L |
0 |
5.81 |
94.1 |
10.1 |
23.2 |
9.12 |
142 |
5.0 mg/L |
72 |
3.76 |
11.1 |
2.32 |
7.12 |
2.82 |
27.1 |
25 mg/L |
0 |
37.6 |
735 |
75.3 |
222 |
96.1 |
1166 |
25 mg/L |
72 |
22.5 |
116 |
22.8 |
84.5 |
38.4 |
285 |
100 mg/L |
0 |
125 |
1111 |
116 |
243 |
113 |
1708 |
100 mg/L |
72 |
104 |
173 |
32.7 |
81.6 |
41.5 |
432 |
Remarks:
* Limit of detection
** derived from equation of the calibration line (axis intercept); no signal was measured in the controls for α-Terpineol, β-Pinene and Limonene
Quantitative GC-MS-values are based on results and/or limits of detection
Description of key information
A 72-h EL50 of 17.1 mg/L and a NOELR of 10
mg/L have been determined for the effect of Crude Sulfate Turpentine on
growth rate of Desmodesmus subspicatus.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 17.1 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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