Di(benzothiazol-2-yl) disulphide

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov 2015 - Jan 2016

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

via HPLC-MS-MS

Details on sampling:

Sampling schedule:
Control : on day 0, 9 and 19 in the freshly prepared media; on day 2, 12 and 21 in the old media
Test concentration : on day 0, 9 and 19 in the freshly prepared media; on day 2, 12 and 21 in the old media

Vehicle:

no

Details on test solutions:

Ensured by renewal of test media three times per week and verified by chemical analysis (HPLC-MS-MS) according to the sampling scheme described below.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, parthenogenetic females

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

21 d

Hardness:

The hardness of the dilution water ranged from 14.0 °dH to 16.0 °dH
(= 250 – 286 mg/L CaCO3) during the test.

Test temperature:

20 +/- 2 °C

pH:

7.6 - 7.8

Dissolved oxygen:

> 3 mg/L throughout exposure period

Nominal and measured concentrations:

10 mg/L (nominal)

Details on test conditions:

A 16 : 8 hour light-dark photoperiod (light intensity: < 20 μE x m-2 x s-1) was applied. The culture water (so-called 'M4 medium') was partly renewed once a week.

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: equal an analytical value of 0.1 μg/L (limit of quantitation).

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: equal an analytical value of 0.1 μg/L (limit of quantitation).

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: equal an analytical value of 0.1 μg/L (limit of quantitation).

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: equal an analytical value of 0.1 μg/L (limit of quantitation).

Details on results:

The criterion of adverse effects used in this study was the item-induced inhibitory effect on the parthenogenetic reproduction rate and the mobility behaviour/mortality rate of parent Daphnia, all effects being assessed at least three times a week.

Validity criteria fulfilled:

yes

Conclusions:

No toxic effects of Rubator MBTS against Daphnia were observed at a limit test concentration of 10 mg/L (nominal) over 21 days, which equals to an analytical value of 0.1 μg/L (limit of quantitation).

Executive summary:

Long-term toxicity on invertebrates were investigated according to OECD 211 over 21 days under semi static conditions in a daphnia reproduction test. Analysis was performed via HPLC-MS-MS. As a result, Rubator MBTS showed no toxic effect in a limit test with a nominal concentration of 10 mg/L which equal an analytical value of 0.1 µg/L which was the limit of quantification (Currenta, 2016). Therefore a 21d-NOEC of >= 10 mg/L (reproduction and mortality) and 21d-LOEC of > 10 mg/L (reproduction and mortality) could be determined.

Description of key information

No toxic effects of Rubator MBTS against Daphnia were observed at a limit test concentration of 10 mg/L (nominal) over 21 days, which equals to an analytical value of 0.1 μg/L (limit of quantitation).