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Diss Factsheets
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EC number: 201-195-7 | CAS number: 79-31-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given, comparable to guideline study with acceptable restrictions (no analytical monitoring)
- Qualifier:
- according to guideline
- Guideline:
- other: German industrial standard test guideline DIN 38412, part 11
- Principles of method if other than guideline:
- Method used is comparable to OECD TG 202 (Daphnia sp., Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique Appliquee" and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation (mean and range, SD): 2-24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.63 mmol/L (total hardness)
- Test temperature:
- 20 °C
- pH:
- lowest test substance concentration: 7.89
highest test substance concentration: 4.28
pH-values were nearly stable over the test period - Dissolved oxygen:
- 8.16 - 9.2
- Nominal and measured concentrations:
- Nominal test concentrations (mg/L): control, 15.625, 31.3, 62.5, 125, 250, 500
- Details on test conditions:
- TEST SYSTEM
- Test vessel: reagent tubes with flat bottom, 10 ml test volume
- Type (delete if not applicable): open / closed
- Fill volume: 20 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater.
- Ks up to pH 4.3:= 0.9 ± 0.1 mM
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement: pH values after 0 and 48 h
OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours day night regime
- Light intensity: 5 - 6 µE/(m2 * s) (400 -750 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 3, 6, 24 and 48 hours - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 31.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 38.28 - 64.22 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0 / 20
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a Daphnia immobilization test, the LC50 of isobutyric acid was determined to be 51.25 mg/L.
- Executive summary:
Isobutyric acid was tested on the waterflea Daphnia magna in a 48-h acute toxicity test similar to OECD TG 202 under static conditions at concentrations of 0 (water control), 15.625, 31.3, 62.5, 125, 250, and 500 mg/L. Immobilization and other toxic effects were observed 3, 6, 24, and 48 h after initiation of the test.
The 48-h EC50 was determined to be 51.25 mg/L (BASF AG, 1989).
This acute toxicity study with Daphnia is acceptable and satisfies the information requirements for an acute toxicity study with freshwater invertebrates.
Reference
Mobility of Daphnids
Concentration |
Number of mobile test animals after |
|||
3 h |
6 h |
24 h |
48 h |
|
0 (controls) |
20 |
20 |
20 |
20 |
15.625 |
20 |
20 |
20 |
20 |
31.3 |
20 |
20 |
20 |
20 |
62.5 |
20 |
20 |
15 |
4 |
125 |
14 |
7 |
0 |
0 |
250 |
0 |
0 |
0 |
0 |
500 |
0 |
0 |
0 |
0 |
Description of key information
In a valid Daphnia acute immobilization test similar to OECD TG 202, the EC50 of isobutyric acid was determined to be 51.25 mg/L (BASF AG, 1989)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 51.25 mg/L
Additional information
This endpoint is covered by two studies. One study is a valid 48-h acute Daphnia immobilization test (BASF AG, 1989). The second study (Hüls, 1979) cannot be used for assessment of this endpoint due to critical deviations from requirement of actual test guidelines (test period only 24 h).
BASF AG 1989
Isobutyric acid was tested on the waterflea Daphnia magna in a 48-h acute immobilization test similar to OECD TG 202 under static conditions at concentrations of 0 (water control), 15.625, 31.3, 62.5, 125, 250, and 500 mg/L. Immobilization and other toxic effects were observed 3, 6, 24, and 48 h after initiation of the test.
The 48-h EC50 was determined to be 51.25 mg/L (BASF AG, 1989).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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