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EC number: 939-529-5 | CAS number: 1471313-51-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-11-15 to 2012-11-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study compliant with GLP, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acid chlorides, C12-18 (even numbered) and C18 unsatd., reaction products with sodium N-methyltaurinate
- EC Number:
- 939-529-5
- Cas Number:
- 1471313-51-5
- Molecular formula:
- not applicable
- IUPAC Name:
- Fatty acid chlorides, C12-18 (even numbered) and C18 unsatd., reaction products with sodium N-methyltaurinate
- Details on test material:
- - Name of test material (as cited in study report): Sodium methyl cocoyl taurate
- Physical state: Slightly yellowish coarse powder, solid
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Directly weighing of the stock solution, 30 min ultrasound treatment at room temperature
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: 100 - 180 - 320 - 560 - 1000 mg/L corresponding to 72.3 - 130 - 231 - 405 - 723 mg a.i./L
- Controls: Triplicates at the beginning of the test + triplicates at the end of the test
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: Washed twice with autoclaved tap water and an adjustment to a dry weight concentration of 3.0 g/L +/- 10 % was not necessary. The sludge was used within 24 h after sampling
- Dry sludge concentration: 3.18 g/L corresponding to 1.59 g/L in the test vessel
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 19.8 °C
- pH:
- pH-value of the activated sludge 7.98
pH-value of the synthetic waste water 7.24 - Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 3
- Composition of test medium:
Test item concentration [mg/L]
100 180 320 560 1000
Inoculum 250
Synthetic waste water 16
Stock solution [g/L] 5
Volume stock solution [mL] 10 18 32 56 100
Demineralised water [mL] 224 216 202 178 134
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Range finding study
Test item concentration pH (test item in water) Inhibition [%]
[mg/L] [mg a.i./L]
10 7.23 - -10
100 72.3 - 11
1000 723 - #
100 72.3 6.61 5
1000 723 6.80 49 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- is UVCB means 100% act.ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 513 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- is UVCB means 100% act.ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95 CI 465-572 mg/L
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 94.6 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test were 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC-values of the test item were calculated by sigmoidal dose-response regression and the EC50-value of the reference item by linear regression using software GraphPad Prism, GRAPHPAD SOFTWARE, INC. Calculation of the confidence intervals for the EC50-values was carried out using standard procedures.
Any other information on results incl. tables
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h.
The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison with the oxygen uptake rates of the control.
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Control and Reference Item Concentrations
Test Item |
Repl. |
Oxygen Uptake Rate |
Specific Respiration Rate |
Inhibition |
Mean Inhibition |
|
[mg/L] |
[mg a.i./L] |
[mg O2/L×h] |
[mg O2/g×h] |
[%] |
[%] |
|
Control |
1 |
24.0 |
15.1 |
– |
– |
|
2 |
28.4 |
17.9 |
||||
3 |
28.4 |
17.9 |
||||
4 |
18.8 |
11.8 |
||||
5 |
20.4 |
12.8 |
||||
6 |
20.4 |
12.8 |
||||
100 |
72.3 |
1 |
24.8 |
-6 |
-1 |
|
2 |
23.2 |
1 |
||||
3 |
23.2 |
1 |
||||
4 |
24.4 |
-4 |
||||
5 |
24.8 |
-6 |
||||
180 |
130 |
1 |
20.0 |
15 |
12 |
|
2 |
21.2 |
9 |
||||
3 |
20.4 |
13 |
||||
4 |
21.2 |
9 |
||||
5 |
20.0 |
15 |
||||
320 |
231 |
1 |
14.8 |
37 |
37 |
|
2 |
14.4 |
38 |
||||
3 |
14.8 |
37 |
||||
4 |
14.0 |
40 |
||||
5 |
14.8 |
37 |
||||
560 |
405 |
1 |
11.6 |
50 |
50 |
|
2 |
11.6 |
50 |
||||
3 |
11.6 |
50 |
||||
4 |
11.2 |
52 |
||||
5 |
12.0 |
49 |
||||
1000 |
723 |
1 |
10.0 |
57 |
57 |
|
2 |
10.0 |
57 |
||||
3 |
10.8 |
54 |
||||
4 |
9.2 |
61 |
||||
5 |
10.8 |
54 |
Repl. = Replicate
Mean specific respiration rate of the control: 14.7 mg O2/g×h
Coefficient of variation of oxygen uptake rates of the control: 18.1 %
Suspended solids in the test vessel: 1.59 g/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item Sodium methyl cocoyl taurate is 180 mg/L, corresponding to 130 mg a.i./L. The test item is not toxic up to the concentration of 180 mg/L, corresponding to 130 mg a.i./L, to activated sludge of a municipal sewage treatment plant. The EC50 is 513 mg/L, corresponding to 371 mg a.i./L.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Sodium methyl cocoyl taurate (batch number: DEB4003323) from 2012-11-15 to 2012-11-16, with the definite study on 2012-11-16 at the Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the substance concentrations 100 - 180 - 320 - 560 - 1000 mg/L, corresponding to 72.3 - 130 - 231 - 405 - 723 mg a.i./L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -1 % to 57 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 94.6 mg/L.
IMPORTANT NOTE TO AVOID CONFUSION:
As the registration substance is a UVCB this means that the test item is 100% active ingredient.
NOEC and EC-Values with Confidence Intervals of Sodium methyl cocoyl taurate
EC-values
[mg/L]Confidence interval
P = 95 [%]
[mg/L]NOEC*
180
–
EC20
215
204 - 226
EC50
513
465 - 572
EC80
> 1000
–
The NOEC of the test item Sodium methyl cocoyl taurate is 180 mg/L.
The test item is not toxic at concentrations up to 180 mg/L
to activated sludge of a municipal sewage treatment plant.
The EC50 of the test item is 513 mg/L.
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