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EC number: 202-267-0 | CAS number: 93-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted and reported according to the OECD Guideline for the testing of chemicals 401 and principles of GLP.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2'-methylacetoacetanilide
- EC Number:
- 202-267-0
- EC Name:
- 2'-methylacetoacetanilide
- Cas Number:
- 93-68-5
- Molecular formula:
- C11H13NO2
- IUPAC Name:
- N-(2-methylphenyl)-3-oxobutanamide
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- TS: Mitsuboshi Chemical Co., Ltd.
Purity: 99.9%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Source: Japan Charles River Co. Ltd.
Age: 5 weeks old
Weight at initiation: 120-137g for males, 106-118g for females
Pellet food: free take till 17:00 on the day before test and from 3 hours after dose onward
Water: free take
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1% Methylcellulose solution
- Details on oral exposure:
- Vehicle: 1% Methylcellose water solution
Route: 1.0mL/100g body weight by gavage
Post dose observation: till 14 days after administration - Doses:
- 0, 819, 1024, 1280, 1600, 2000, 2500 mg/kg for both sexes
- No. of animals per sex per dose:
- 5 animals per sex per dose
- Control animals:
- yes
- Details on study design:
- Post dose observation: till 14 days after administration
No further data available. - Statistics:
- No data available.
Results and discussion
- Preliminary study:
- Not applicable
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD0
- Effect level:
- 1 024 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 854 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 1 549 - 2 298
- Sex:
- male
- Dose descriptor:
- LD100
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD0
- Effect level:
- 1 280 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 945 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 1 654 - 2 318
- Sex:
- female
- Dose descriptor:
- LD100
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- MORTALITY
number of number of deaths
dose animals male female
mg/kg per sex
-----------------------------------------------------------------------------------------------
0 5 0 0
819 5 0 0
1024 5 0 0
1280 5 1(Hr.3) 0
1600 5 0 1(Day3)
2000 5 3(Hr.3, Day3) 2(Hr.3,6)
2500 5 5(Hr.3, Day3) 5(Hr.3,6,Day2,3)
-----------------------------------------------------------------------------------------------
Hr.: hours after dose,
Day: days from dose - Clinical signs:
- other: From 10 minutes after dose, decreased locomotor activity and adoption of prone position were observed in all treated groups, and hypomyotonia, ptosis and deep respiration were observed in many of treated groups. From 1 to 3 hours later, piloerection, hypo
- Gross pathology:
- At necropsy, bloody material in the stomach and intestine, petechiae in the glandular stomach and
distension of the urinary bladder were observed in the animals that died. - Other findings:
- No other findings available.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- The LD50 value by oral for rat is 1854 mg/kg for male and 1945 mg/kg for female.
- Executive summary:
The study was published in the OECD SIDS dossier in year 2003 conducted and reported in year 1999 according to the OECD Guideline for the testing of chemicals 401 and principles of GLP. The first mortality was oberserved at 1280 mg/kg dose level for males and at 1600 mg/kg for females, further deaths at 2000 mg/kg dose level for males and females.
Dead animals showed serious those clinical signs and weak respiration before die.
Therefore, the LD50 value by oral for rat is 1854 mg/kg for male and 1945 mg/kg for female.
The substance will be classified as harmful (H 302) according to GHS.
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