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EC number: 210-323-0 | CAS number: 612-83-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
For the determination of the toxicity of the submission substance 3,3´-dichlorobenzidine dihydrochloride its corresponding base, 3,3´-dichlorobenzidine, being of equivalent toxicological concern, had been tested in short-term toxicity studies in fish, aquatic invertebrates (Daphnia magna), aquatic plants (algae, Scenedesmus subspicatus) and microorganisms.
The key study for fish (reliability category 1) had been performed on Brachydanio rerio (HAMILTON - BUCHANAN) equivalent to OECD 203 fulfilling all the validity criteria of the guideline. The LC50 after 96 hours had been determined to be 3.3 mg/L 3,3´-dichlorobenzidine.
Other LC50 -values for fish species from the secondary literature (ECOTOX-database) are between 1.1 and 2.8 mg/L (all Pimephales promelas) 3,3´-dichlorobenzidine and thus not in contradiction to the key study.
The key study for aquatic invertebrates (reliability category of 1) had been performed on Daphnia magna STRAUS equivalent to OECD 202, fulfilling all the validity criteria of the guideline. The EC50 (immobilization) after 48 hours had been determined to be 2.47 mg/L 3,3´-dichlorobenzidine with a 95% confidence interval of 2.12 - 2.86 mg/L. Another EC50 -value (mortality) for daphnia magna from secondary source (ECOTOX-database) is 1.05 mg/L 3,3´-dichlorobenzidine and thus well in accordance with the result of the key study. The EC0 after 48 h as reported in the key study was 1.14 mg/L.
The key study for aquatic plants (reliability category 1) had been performed on the unicellular alga Scenedesmus subspicatus. In a growth inhibition test according to OECD 201 fulfilling all the validity criteria demanded by OECD 201 as well as the requirements on the reporting on experimental procedures and results, an EC50 (growth reduction) after 72 hours of 4.3 mg/L 3,3´-dichlorobenzidine with a 95% confidence interval of 3.2 - 5.6 mg/L in comparison to the solvent control group had been determined. The no observed effect concentration (NOEC) as defined in the OECD-test guidelines (no significant growth inhibition and no cell deformation) was found after 72 hours to be 0.32 mg/L 3,3´-dichlorobenzidine.
Microorganisms: With the exception of Photobacterium phosphoreum (EC50 light emission = 0.058 mg/L, no incubation duration given), the secondary literature reports effect values well above the solubility limit of 3,3´-dichlorobenzidine in water (3 mg/L) or no effect at all for activated sludge bacteria and several single strains.
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