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EC number: 212-298-1 | CAS number: 778-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 – 16 Mar 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes
- Remarks:
- The test was performed in compliance with the requirements of the Principles of Good Laboratory Practices (GB/T 22278-2008).
Test material
- Reference substance name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- EC Number:
- 212-298-1
- EC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- Cas Number:
- 778-94-9
- Molecular formula:
- C8H3F3N2O2
- IUPAC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 1.32, 1.98, 2.97, 4.45, and 6.67 mg/L
- Sampling method: Water samples were taken at 0, 24, 48, 72, and 96 h
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.2020 g was weighed, and test water used to dilute to 2 L, then stirred continuously for 30 minutes at 500 rpm at room temperature (23-25 °C), yielding the stock solution with a 100 mg/L concentration.
- Other relevant information: The test substance was fully dissolved.
Test organisms
- Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: rare minnow
- Strain: Batch number: F20181121G
- Source: Institute of Hydrobiology of the Chinese Academy of Sciences
- Length at study initiation (length definition, mean, range and SD): 3.37 cm (SD 4.04%)
- Weight at study initiation (mean and range, SD): 0.257 g (SD 8.38%)
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): The test fish underwent preliminary rearing in aerated, circulating water for a minimum of 12 days or more, during which period fish with abnormal growth or dead fish were removed. Prior to testing, they were acclimated in the test environment for 7 days.
- Type and amount of food during acclimation: They were fed newly hatched Artemia salina
- Feeding frequency during acclimation: Twice daily, terminating 24 h prior to the start of the test.
FEEDING DURING TEST
- Food type: No feeding
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 148 mg (CaCO3)/L
- Test temperature:
- 22.6 - 23.5 °C
- pH:
- 7.82 - 8.29
- Dissolved oxygen:
- 68.4 - 104.6%
- Nominal and measured concentrations:
- Nominal concentrations: 0.00 (Control), 1.32, 1.98, 2.97, 4.45, and 6.67 mg/L
Mean measured concentrations: < LOD/LOQ, 1.28, 1.95, 2.91, 4.30, and 6.62 mg/L - Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: 0.257 g (wet weight)/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water aerated for 24 h or more
- Metals: Arsenic 0.5 μg/L; Cadmium <0.5 μg/L; Lead <2.5 μg/L; Cobalt < 0.02 mg/L; Nickel < 0.05 mg/L; Mercury 0.10 μg/L; Copper 5.2 μg/L; Chromium6+ 0.004 mg/L; Iron <0.01 mg/L; Zinc < 0.09 mg/L; Silver < 0.03 mg/L
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Photoperiod: 12 h light each day
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Every day, test temperature, pH, and dissolved oxygen water quality parameters were measured and recorded.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study: yes
- Test concentrations: Control, 0.100, 1.00, and 10.0 mg/L
- Results used to determine the conditions for the definitive study: Maximum concentration causing zero mortality in the test fish (LC0) and minimum concentration causing 100% mortality in the test fish (LC100) were recorded. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.47 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.28 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: At concentrations 2.97 mg/L and higher fish lay flat or side-on. After 96 h of exposure, the test fish in the 2.97 mg/L, 4.45 mg/L, and 6.67 mg/L treatment groups were all dead.
- Mortality of control: Swimming behaviour of the test fish in the blank control group was normal and there were no deaths. - Results with reference substance (positive control):
- - Results with reference substance were valid.
- Mortality: Up to a potassium dichromate concentration of 200 mg/L no mortality was observed.
- LC50: The potassium dichromate 24 h-LC50 in the test fish was between 200 mg/L - 400 mg/L.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Measurement Results for Stability of the Test Substance in Test Medium
Prepared concentration (mg/L)
Measured concentration (mg/L)
0 h
24 h
48 h
72 h
96 h
Mean
0
ND(1)
ND
ND
ND
ND
-
1.32
1.33
1.31
1.28
1.26
1.23
1.28
1.98
2.00
1.97
1.96
1.94
1.88
1.95
2.97
3.00
2.96
2.91
2.88
2.80
2.91
4.45
4.41
4.33
4.28
4.16
-
4.30
6.67
6.71
6.59
6.55
-
-
6.62
(1) ND: lower than the limit of detection
- : 100% mortality in test fish not detected.
Table 2: Test Fish Fatalities
Prepared concentration (mg/L)
Measured concentration
(mg/L)
Number of fish (n)
Number of dead fish (n)
3h
6h
24 h
48 h
72 h
96 h
0
ND
10
0
0
0
0
0
0
1.32
1.28
10
0
0
0
0
0
3
1.98
1.95
10
0
0
1
1
8
9
2.97
2.91
10
0
0
1
9
10
10
4.45
4.30
10
0
0
5
10
10
10
6.67
6.62
10
0
2
10
10
10
10
LC50 (mg/L) (based on actual measured concentration)
4.01
2.38
1.72
1.47
95% confidence interval (mg/L) (based on actual measured concentration)
3.38-4.75
2.13-2.65
1.55-1.90
1.28-1.70
Table 3: Validity criteria for OECD 203 (2019)
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
Mortality rate in the blank control group was 0%.
yes
The dissolved oxygen concentration must have been at least 60% of the air saturation value throughout the test
Dissolved oxygen: greater than 68.4% of the air saturation value.
yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20%, results should be based on the measured concentration.
Change in concentration was less than 7% of the initial concentration.
yes
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 in "Any other information on results incl. tables".
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