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EC number: 204-800-2 | CAS number: 126-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Published public domain study
- Qualifier:
- according to guideline
- Guideline:
- other: FEA 1984 - Provisional Procedure: extended toxicology test with Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of first appearance of offspring; 21d)
- Deviations:
- yes
- Remarks:
- chemical determinations of substance concentrations were modified for reasons of practicality
- Principles of method if other than guideline:
- The 21-d reproduction test was utilised to determine the effect of tributyl phosphate on Daphnia. A semi-static method was used to determine the NOEC and lethal concentration on the parent Daphnia.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant. - Analytical monitoring:
- yes
- Details on sampling:
- Concentrations: samples were taken twice from selected concentration levels of the test series during the test period and analysed chemically. The first sample was taken before the 7th day (in the period during which no offspring appeared), and the second sample was taken between the 16th and 21st day.
- Vehicle:
- not specified
- Details on test solutions:
- The test substance was dissolved in synthetic fresh water (dilution water) using magnetic stirrers for 1 hour.
Graduated dilutions using dilution water were produced in the concentration range in which effects were expected, based on the 24h Daphnia test and a preliminary 3d Daphnia test.
Controls were produced that contained no test substance. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia magna IRCHA strain was used in the test. The strain had been maintained in accordance with the procedure practised since 1978.
20-30 specimens were placed in forty 2l beakers which had been filled with at least 1.6l Berlin tap water. They provided 24 hour old animals when the offspring were removed daily from the cultures.
Cultures were maintained in dechlorinated and oxygen-saturated tap water (which had been left to stand for 24h) kept at 20 °C. They were kept in glass beakers on a white surface, under exclusion of daylight but lit with fluorescent lamps for 9 hours a day.
Feeding took place daily with dry algae from the Scenedesmus genus; 9g feed suspended in 1L tap water, 2ml of this suspension was added to each culture beaker. Feeding continued throughout the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- No information.
- Hardness:
- The amount of calcium and magnesium ions in the dilution water was 2.5mmol/L.
- Test temperature:
- 25 ± 1 °C.
- pH:
- 8.0 ± 0.2
- Dissolved oxygen:
- The water was aerated up to the water saturation level.
- Salinity:
- Not relevant.
- Nominal and measured concentrations:
- No information provided.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open (beakers covered with watch glasses)
- Material, size, headspace, fill volume: 400ml glass beaker with 250ml useful capacity
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): Semi-static procedure, parent animals transferred to fresh solution 3 times a week
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (see below)
- Metals: Molar relationship of sodium to potassium ions was 10:1
- Ca/mg ratio: 2.5mmol/l
- Conductivity: when conductivity was <1µS/com, the dilution water was diluted with 10% tap water
- Culture medium different from test medium: yes; culture medium was 20 °C dechlorinated and oxygen saturated tap water which had been left to stand for 24 hours. pH 7.6-7.7, German hardness 16°.
OTHER TEST CONDITIONS
- Adjustment of pH: pH valure measured as 8.0±0.2
- Photoperiod: 9 hours fluorescent light (7am to 4pm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 24h EC0 & EC50, 21d NOEC - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- other: LC0
- Effect conc.:
- 9.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- No further information
- Results with reference substance (positive control):
- Not relevant.
- Reported statistics and error estimates:
- The student's t-test and the U-test were used when calculating the NOEC from the mortality of the parent animals and the reproduction rate.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The NOEC was determined to be 1.3mg/L in the 21-day reproduction test.
- Executive summary:
The 21-day reproduction test was carried out in Daphnia magna to determine the NOEC for tributyl phosphate. Evaluation parameters for fixing the NOEC were parent animal mortality, reproduction rate and the appearance of the first offspring during the test period. Animals were aged 24 hours at the start of the test. A semi-static procedure was used with open vessels maintained under fluorescent lighting, animals were fed daily with algae suspension (the test was carried out in accordance with an FEA guideline). The 24 hour EC50 value was also determined. The NOEC was found to be 1.3 mg/L in the 21-day reproduction test.
Reference
The NOEC was determined to be 1.3 mg/L in the 21-day reproduction test.
Description of key information
One proprietray and one published study are available.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 1.3 mg/L
Additional information
A 21-day reproduction test was carried out in Daphnia magna to determine the NOEC for tributyl phosphate (Kuehn et al, 1989). Evaluation parameters for fixing the NOEC were parent animal mortality, reproduction rate and the appearance of the first offspring during the test period. Animals were aged 24 hours at the start of the test. A semi-static procedure was used with open vessels maintained under fluorescent lighting, animals were fed daily with algae suspension (the test was carried out in accordance with an FEA guideline). The 24 hour EC50 value was also determined. The NOEC was found to be 1.3 mg/l in the 21 day reproduction test.
The 21 day EC50 (immobilisation) of tributyl phosphate in Daphnia magna is also reported to be >2.1mg/l (ABC Laboratories, 1991).
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