Octanoyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Octanoyl chloride

EC Number:

203-891-6

EC Name:

Octanoyl chloride

Cas Number:

111-64-8

Molecular formula:

C8H15ClO

IUPAC Name:

octanoyl chloride

Details on test material:

- Name of test material (as cited in study report): n-Octansaeurechlorid
- Physical state: Colorless liquid
- Analytical purity: 97% (by GC)
- Lot/batch No.: Vers. 62/V
- Storage condition of test material: Room temperature

Method

Target gene:

his- (S. typhimurium)
trp- (E. coli)

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254 induced rat liver S-9 mix

Test concentrations with justification for top dose:

4.0 µg - 1000 µg/plate (SPT)
0.8 µg - 500 µg/plate (PIT)

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: see table

Details on test system and experimental conditions:

Experiment 1: Standard plate test (SPT)
Test tubes containing 2 ml soft agar kept in a water bath at 45°C, and remaining components added in the following order:
0 .1 ml test solution or vehicle
0 .1 ml bacterial suspension
0 .5 ml S-9 mix (in tests with metabolic activation) or 0 .5 ml phosphate buffer (in tests without metabolic activation).
After mixing, the samples are poured onto Vogel-Bonner agar plates.

Experiment 2: Preincubation test (PIT)
0.1 ml test solution or vehicle, 0.1 ml bacterial suspension and 0 .5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes. Subsequently, 2 ml of soft agar is added
and, after mixing, the samples are poured onto the Vogel-Bonner agar plates.

Experiment1&2
In each experiment 3 test plates per dose or per control used; after incubation at 37°C for
48 hours in the dark, the bacterial colonies ( his+ revertants) are counted.
Positive control:
with metabolic activation: 2.5 μg/plate 2-aminoanthracene for each S. typhimurium strain; 60 μg/plate 2-aminoanthracene for the E. coli strain
without metabolic activation: 5 μg/plate N-methyl-N'-nitro-N-nitrosoguanidine for TA 100 and TA 1535, 10 μg/plate 4-nitro-o-phenylendiamine
for TA 98, 100 μg/plate 9-aminoacridine chloride monohydrate for TA 1537 and 10 µg/plate N-Ethyl-N-nitro-N-nitrosoguanidine for E. coli WP2 uvrA, all substances were dissolved in DMSO.
The titer was determined and in regularly measurements the strain characteristics were
checked. Sterility control was performed.

Evaluation criteria:

Positive results
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the result

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

A bacteriotoxic effect was observed at doses >= 500 µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

A bacteriotoxic effect was observed at doses >= 500 µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Standard plate test (4 - 1000 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 98	no	32	0.9	no	negative
	yes	42	1.1	no	negative
TA 100	no	125	1.1	no	negative
	yes	127	1.3	no	negative
TA 1535	no	21	1.0	no	negative
	yes	20	1.0	no	negative
TA 1537	no	9	1.2	no	negative
	yes	9	1.2	no	negative
WP2 uvr A	no	33	1.3	no	negative
	yes	37	1.1	no	negative
Preincubation test (0.8 - 500 µg/plate)
Strain	Metabolic activation system	mean revertants in Controls	maximum revertant factor	dose dependency	Assessment
TA 98	no	29	1.0	no	negative
	yes	45	0.9	no	negative
TA 100	no	139	1.0	no	negative
	yes	136	1.3	no	negative
TA 1535	no	23	0.8	no	negative
	yes	24	0.8	no	negative
TA 1537	no	11	1.1	no	negative
	yes	12	0.9	no	negative
WP2 uvr A	no	32	1.0	no	negative
	yes	36	0.9	no	negative