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EC number: 200-819-5 | CAS number: 74-88-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 April 2000 to 6 June 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Version / remarks:
- 1998
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF, 59 NouSan No. 4200
- Version / remarks:
- 1985
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Iodomethane
- EC Number:
- 200-819-5
- EC Name:
- Iodomethane
- Cas Number:
- 74-88-4
- Molecular formula:
- CH3I
- IUPAC Name:
- iodomethane
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report): Iodomethane
- Physical state: liquid
- Analytical purity: 99.7%
- Impurities (identity and concentrations): 0.2 % water and <0.1 % methanol
- Composition of test material, percentage of components: not stated
- Isomers composition: not stated
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Females nulliparous and non-pregnant: yes
- Age at study initiation: range-finder: males and females-11 weeks, main study: males-10 to 13 weeks and females-10 to 12 weeks
- Weight at study initiation: range-finder: males-306 to 358 g and females- 205 to 216 g, main study: males-283 to 399 g and females- 208 to 270 g
- Fasting period before study: overnight before dosing
- Housing: The animals were housed individually in suspended stainless steel cages.
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: a minimum of 5 days
ENVIRONMENTAL CONDITIONS
- Temperature: 18 to 22 °C
- Humidity: 37 to 59 %
- Air changes: room ventilation was set to produce 10-15 air changes/hour.
- Photoperiod: Light timers were set to maintain a 12-hour light/12-hour dark cycle
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- DOSAGE PREPARATION:
- The test material was administered as received or mixed with corn oil to produce a 10 % w/v concentration and dispensed fresh on the day of dosing. The preparations were stirred continuously during dosing.
- The density of the test material was determined twice to confirm the volatile nature of the test material. The average of the two density measurements resulted in 2.22 g/mL. The density of the test material (bulk) was determined to be 2.22 g/mL.
- For the range finding study the test material was dosed as supplied for all concentrations. The maximum volume applied was 2.25 mL/kg
- For the main LC50 study at 50 and 75 mg/kg the test material was dosed from the 10 % w/v dilution prepared in corn oil. For all other dose groups the test material was dosed as supplied (100 %).
- Rationale for the selection of the starting dose: The range-finding data indicated that the test material produced mortality at the 500 mg/kg level in males and in females. Delayed mortality (day 5) was observed at the lowest dose level tested (100 mg/kg) in males, but no mortality occurred in the females. - Doses:
- Range finding study: 100, 500, 1250, 2500 and 5000 mg/kg bw
Main LC50 study: 50, 75, 85, 100, 150, 250, 350 mg/kg bw - No. of animals per sex per dose:
- - Range finding study: 1 animal per sex per dose
- Main LC50 study:
50 mg/kg: 5 animals per sex per dose
75 mg/kg: 5 males
85 mg/kg: 5 males
100 mg/kg: 5 animals per sex per dose
150 mg/kg: 5 females
250 mg/kg: 5 animals per sex per dose
350 mg/kg: 5 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed for clinical abnormalities a minimum of two times on study day 0 (post-dose) and daily thereafter (days 1-14). A general
health/mortality check was performed twice daily (in the morning and in the afternoon).
- Individual body weights were obtained for the study animals prior to fasting (day -1), prior to dosing on day 0 and for all surviving animals on days 7 and 14.
- Necropsy of survivors performed: yes, all study animals which died spontaneously during the study or were euthanised by carbon dioxide inhalation at study termination (day 14) were necropsied. Body cavities (cranial, thoracic, abdominal and pelvic) were opened and examined. All gross lesions were collected and retained in 10 % neutral buffered formalin for possible future histological evaluation. - Statistics:
- The LD50 and 95 % confidence intervals were calculated separately for each sex using a computer adaptation of the method of Litchfield and Wilcoxon.
Results and discussion
- Preliminary study:
- The range-finding data indicated that the test material produced mortality at the 500 mg/kg level in males and in females. Delayed mortality (day 5) was observed at the lowest dose level tested (100 mg/kg) in males, but no mortality occurred in the females.
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 79.84 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 77.25 - <= 82.55
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 131.98 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 95.35 - <= 182.7
- Mortality:
- - All male rats died at the 85 mg/kg dose. No males died at the 75 mg/kg dose.
- For females, all rats died at the 350 mg/kg high dose but none at the 50 mg/kg low dose. Partial deaths were observed at the intermediate doses.
- All mortality occurred by study day 2.
- A summary is shown in Table 1. - Clinical signs:
- other: - The most notable clinical abnormalities observed during the study included breathing abnormalities, prostration, tremors, soft stools/few faeces, faecal stain, dilated pupils, skin pale in colour, ocular discharge, salivation, decreased activity, wobbly
- Gross pathology:
- - The most notable gross internal findings were observed in the animals that died and included abnormal content of the digestive tract, foci/thickening of the stomach, mottled and/or dark red lung lobes, foci on the thymus and blackish-purple lobes of the liver. No significant gross internal findings were observed at necropsy on study day 14 .
Any other information on results incl. tables
Table 1: Summary of mortalities
Dose level (mg/kg) |
Sex |
No. of animals |
Study day |
Mortality |
|||
0 |
1 |
2 |
3 to 14 |
||||
50 |
Male |
5 |
0 |
0 |
0 |
0 |
0/5 |
75 |
5 |
0 |
0 |
0 |
0 |
0/5 |
|
85 |
5 |
4 |
1 |
- |
- |
5/5 |
|
100 |
5 |
4 |
0 |
1 |
- |
5/5 |
|
250 |
5 |
5 |
- |
- |
- |
5/5 |
|
50 |
Female |
5 |
0 |
0 |
0 |
0 |
0/5 |
100 |
5 |
1 |
0 |
0 |
0 |
1/5 |
|
150 |
5 |
3 |
0 |
1 |
0 |
4/5 |
|
250 |
5 |
4 |
0 |
0 |
0 |
4/5 |
|
350 |
5 |
5 |
- |
- |
- |
5/5 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Category 3 according to EU criteria.
- Conclusions:
- Under the conditions of this study, the acute oral LD50 was determined to be 79.84 mg/kg and 131.98 mg/kg in male and female rats respectively.
- Executive summary:
The acute oral toxicity of the test material was determined in accordance with the standardised guidelines EPA OPPTS 870.1100 and JMAFF 59 NohSan No. 3850, under GLP conditions.
The single-dose oral toxicity of the test material was evaluated in Sprague-Dawley rats. An LD50 study was performed in which 5 groups of five male and five female rats received a single oral administration of the test material at graded dosage levels. Following dosing, the LD50 study rats were observed daily and weighed weekly. A gross necropsy examination was performed on all LD50 study animals at the time of death or scheduled euthanasia (day 14).
All male rats died at the 85 mg/kg dose. No males died at the 75 mg/kg dose. For females, all rats died at the 350 mg/kg high dose but none at the 50 mg/kg low dose. Partial deaths were observed at the intermediate doses. All mortality occurred by study day 2. The most notable clinical abnormalities observed during the study included breathing abnormalities, prostration, tremors, soft stools/few faeces, faecal stain, dilated pupils, skin pale in colour, ocular discharge, salivation, wobbly gait, decreased activity, eyelids partially closed, and dark material around the facial area. Body weight gain/maintenance was noted for all surviving animals during the test period. The most notable gross internal findings were observed in the animals that died and included abnormal content of the digestive tract, foci/thickening of the stomach, mottled and/or dark red lung lobes, foci on the thymus and blackish-purple lobes of the liver. No significant gross internal findings were observed at necropsy on study day 14.
Under the conditions of this study, the acute oral LD50 was determined to be 79.84 mg/kg and 131.98 mg/kg in male and female rats respectively.
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