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EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 July 2002 to 15 November 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: final effluent stage at Severn Trent Water plc sewage treatment plant at Loughborough, Leicestershire, UK
- Storage conditions: filtered through course filer paper (first 200 mL discarded) and maintained on aeration at 21 ± 1 ºC - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
SOLUTION A
KH2PO4: 8.50 g/L
K2HPO4: 21.75 g/L
Na2HPO4.2H2O: 33.40 g/L
NH4Cl: 0.50 g/L
SOLUTION B
CaCl2: 27.50 g/L
SOLUTION C
MgSO4.7H2O: 22.50 g/L
SOLUTION D
FeCl3.6H2O: 0.25 g/L
To 1 L of purified water was added 10 mL of solution A and 1 mL each of solutions B to D.
TEST SYSTEM
The following were prepared and inoculated in 500 mL bottles:
(a) four replicates containing inoculated culture medium to act as the control. These bottles contained 5 mL of inoculum and 495 mL of culture medium.
(b) three replicates containing inoculated culture medium and aniline at a concentration of 100 mg/L.
(c) three replicates containing inoculated culture medium and the test substance at a concentration of 50 mg/L.
(d) two replicates containing inoculated culture medium, aniline at a concentration of 100 mg/L and test substance at a concentration of 50 mg/L.
All bottles were inoculated with the prepared inoculum at a rate of 1 % v/v.
- Culturing apparatus: sample flask sealed by a sensor head/CO2 trap immersed in a temperature controlled water bath.
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
On day 28, two control, one standard material, two test material and one toxicity control vessel that were considered to have given the most consistent BOD values were selected for pH and DOC analysis.
STATISTICAL METHODS:
A Students t-test was carried out for the control and test substance vessels to determine any statistically significant differences between the BOD values on day 28 for the control and test groups. - Reference substance:
- aniline
- Preliminary study:
- An initial experiment in which dispersed test material was exposed to sewage sludge microorganisms at a concentration of 100 mg/L showed no biodegradation in the toxicity control vessel (test material plus the standard material, aniline) after 14 days. However, results obtained from the standard material vessel indicated that sufficient biodegradation (68 %) had occurred after 14 days, therefore satisfying the validation criterion given in the test guidelines and confirming the suitability of the inoculum used and culture conditions.
Under the definition given in the test guidelines, this would indicate that the test material, at a concentration of 100 mg/L, exhibited an inhibitory effect on the sewage sludge microorganisms. However results obtained from the test material and toxicity control vessels indicated that although no biodegradation had occurred, the oxygen consumption values obtained were similar to those obtained from the control vessels. This suggests that although the test material was exhibiting an inhibitory effect on biodegradation of the standard material in the toxicity control vessel, as the oxygen consumption values in the test material vessels are similar to those in the control vessels, the test material was not exhibiting a toxic effect. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Following the recommendations of the test guidelines, dispersed test material was exposed in the definitive test to sewage sludge micro-organisms at a reduced test concentration of 50 mg/L with culture medium in sealed culture vessels in the dark at 21 ± 0.7 °C for 28 days. The degradation of the test material was assessed by the measurement of daily oxygen consumption values. Control solutions with inoculum and the standard material, aniline, together with a toxicity control were used for validation purposes.
No degradation of the test material was observed after 28 days and therefore it cannot be considered to be readily biodegradable. - Results with reference substance:
- The standard material vessels indicated that sufficient biodegradation (68 %) had occurred after 14 days, therefore satisfying the validation criterion given in the test guidelines and confirming the suitability of the inoculum used and culture conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the conditions of the test, no degradation of the test material was observed after 28 days and the test material is not considered to be readily biodegradable.
- Executive summary:
In a GLP compliant ready biodegradability study conducted in line with standardised guidelines OECD 301F, EU Method C.4 -D and EPA OPPTS 835.3110, the biodegradability of the test material was determined. Under the conditions of the test, no degradation of the test material was observed after 28 days and the test material is not considered to be readily biodegradable.
Reference
Description of key information
The test material was determined to be not ready biodegradable according to a study performed in line with OECD Guideline 301F, EU Method C.4-D and EPA OPPTS 835.3110.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
See 'Environmental Fate and Pathways' endpoint summary.
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