Ethylenediamine, salt with phosphoric acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization Study of test material on Daphnia magna in a Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

180 mg of CaCO3

Test temperature:

20±2

pH:

7.4

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 12.5 - < 25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: The EC50 > 12.5 mg/L but < 25 mg/L.

9.1. Immobility:

Nominal	Number	of	Immobility
Concentration	Daphnids	0 h	24 h	48 h
(mg/l)
Control	10	-	-	-
1.5625	10	-	-	-
3.125	10	-	-	-
6.25	10	-	-	-
12.5	10	-	1	1
25	10	-	3	6

9.2. pH and oxygen content:

Nominal Concentration Number of		pH(mg/L)
(mg/l)	daphnids	0h	48h
Control	10	7.4	7.2
1.5625	10	7.7	7.1
3.125	10	7.8	7.2
6.25	10	7.5	7.7
12.5	10	7.7	7.4
25	10	7.7	7.4

Nominal Concentration Number of		DO(mg/L)
(mg/l)	daphnids	0h	48h
Control	10	8.6	6.9
1.5625	10	8.7	6.9
3.125	10	8.4	6.3
6.25	10	8.3	6.4
12.5	10	8.2	6.2
25	10	7.3	6.5

9.3. Test temperature:

Nominal Concentration Number of		Temparature
(mg/l)	daphnids	0h	48h
Control	10	21.4	21.1
1.5625	10	21.5	21.1
3.125	10	21.5	21.2
6.25	10	21.5	21.2
12.5	10	21.5	21.3
25	10	21.5	21.4

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be > 12.5 mg/L but < 25 mg/L mg/L.

Executive summary:

The effect of test chemical was studied by taking the daphnia magna as the animal model. Daphnia sp., Acute Immobilization Test was conducted according to OECD Guideline 202. The stock solution was prepared by dissolving test chemical of 200 mg in 200ml of ADAMS media. The test concentration selected for the exposure was 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively. The study was conducted in static conditions for the period of 48 hours and observed for any occurance immobilization. The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Short-term toxicity to aquatic invertebrates:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium butanedioate. The test substance was soluble in ADaMs’ media. The in-house water solubility of test substance in milli Q water was found to be 5509.96 mg/L. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively.

 The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

574 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium butanedioate. The test substance was soluble in ADaMs’ media. The in-house water solubility of test substance in milli Q water was found to be 5509.96 mg/L. Therefore, the stock solution was prepared by dissolving 200 mg of the test substance in 200 ml of ADaMs’’ media with continuous 15 minutes of stirring and the concentration of stock solution was analytically detected and then the test solution was prepared foe achieving test concentrations of 1.5625mg/L,3.125mg/L,6.25mg/L,12.5mg/L,25mg/L, respectively. The median lethal concentration (EC50) for test substance on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 12.5 mg/L but < 25 mg/L mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, exhibit short term toxicity to Daphnia and can be classified as aquatic chronic 3. But as the chemical was readily biodegradable in water, thus on the basis of readily biodegradable nature of chemical, chemical consider to be nontoxic and not classified as per the CLP classification criteria.