p-[(2-chloroethyl)ethylamino]benzaldehyde

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

occlusive coverage

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-[(2-chloroethyl)ethylamino]benzaldehyde

EC Number:

220-150-2

EC Name:

p-[(2-chloroethyl)ethylamino]benzaldehyde

Cas Number:

2643-07-4

Molecular formula:

C11H14ClNO

IUPAC Name:

4-[(2-chloroethyl)(ethyl)amino]benzaldehyde

Details on test material:

N-Ethyl-N-(2-chlorethyl)-p-amino-benzaldehyd (purity> 90 %)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

other: no vehicle or aqua dest.

Details on dermal exposure:

TEST SITE
- Area of exposure: 50 cm²

Duration of exposure:

24 h

Doses:

200, 400, 1000, 1470, 2000, 2610, 3160, 4640 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

1 630 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 4 600 mg/kg bw

Mortality:

Mortality was observed in the dose 4640, 3160, 2610, 2000, 1470 mg/kg b.w.

Clinical signs:

other: dyspnea, apathy, excitation, abdominal position, staggering, atonia, tremor, spastic gait, saltation convulsions, bite convulsions, tonic convulsions, tonic-clonic convulsions, anemic paleness, poor general state, piloerrection

Gross pathology:

Dead animals: heart: dilatation right side; acute congestive hyperemia; liver: almost loamy grey due to broadened grey hepatic lobules
Sacrificed animals: nothing abnormal detected

Other findings:

LOCAL SKIN IRRITATIONS:
4640 mg/kg bw: after 24 h slight erythema, slight edema, after 7 days nothing abnormal detected
3160 mg/kg bw: after 24 h questionable slight erythema, slight edema, after 7 days partial scaling, after 13 days yellow substance residuals (male animals), female animals after 13 days: no abnormality detected
2610 mg/kg bw: after 24 h questionable slight erythema, slight edema, after 7 days nothing abnormal detected
2000 mg/kg bw: after 24 h slight erythema, slight edema, after 7 days scaling, after that nothing abnormal detected
1470 mg/kg bw: after 24 h questionable erythema, very slight edema, after 7 days nothing abnormal detected
1000 mg/kg bw: after 24 h slight edema, strong edema, after 7 days nothing abnormal detected
400 mg/kg bw: after 24 h slight erythema, slight edema, after 7 days nothing abnormal detected
200 mg/kg bw: after 24 h questionable erythema, slight edema, after 7 days nothing abnormal detected

Any other information on results incl. tables

Mortality data:

Dose (mg/kg)	Conc. (%)	No. of animals	died within
			1 hour	24 hours	48 hours	7 days	14 days
4640	100	4 males	0/4	1/4	1/4	1/4	1/4
4640	100	5 females	0/5	4/5	4/5	5/5	5/5
3160	100	4 males	0/4	0/4	0/4	0/4	0/4
3160	100	5 females	0/5	3/5	3/5	3/5	3/5
2610	100	5 males	0/5	0/5	0/5	1/5	1/5
2610	100	5 females	0/5	3/5	3/5	3/5	3/5
2000	100	5 males	0/5	0/5	0/5	0/5	0/5
2000	100	5 females	0/5	1/5	2/5	3/5	3/5
1470	100	5 males	0/5	0/5	0/5	0/5	0/5
1470	100	5 females	0/5	4/5	4/5	4/5	4/5
1000	100	5 males	0/5	0/5	0/5	0/5	0/5
1000	100	5 females	0/5	1/5	1/5	1/5	1/5
400	50	5 males	0/5	0/5	0/5	0/5	0/5
400	50	5 females	0/5	0/5	0/5	0/5	0/5
200	25	5 males	0/5	0/5	0/5	0/5	0/5
200	25	5 females	0/5	0/5	0/5	0/5	0/5