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EC number: 500-322-6 | CAS number: 144086-03-3 1 - 6.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Glycerol, ethoxylated, esters with acrylic acid
- EC Number:
- 500-322-6
- EC Name:
- Glycerol, ethoxylated, esters with acrylic acid
- Cas Number:
- 144086-03-3
- Molecular formula:
- UVCB substance
- IUPAC Name:
- Glycerol, ethoxylated, esters with acrylic acid
- Details on test material:
- - Name of test material (as cited in study report): Glycerin3EOTA
- Physical state: Beige, turbid liquid
- Analytical purity: >99%
- Lot/batch No.: GK0561/160
- Stability under test conditions: The stability of the test substance under storage conditions throughout the study period is guaranteed until June 15, 2005 as indicated by the sponsor, and the sponsor holds this responsibility
- Storage condition of test material: Room temperature
Constituent 1
Method
- Target gene:
- S. typhimurium: his-
E. coli: trp-
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 1535, TA 100, TA 1537, TA 98; E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S-9 mix from Aroclor 1254 induced male rats
- Test concentrations with justification for top dose:
- 0; 20; 100; 500; 2500 and 5000 µg/plate in both the SPT and the PT
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: see below
- Details on test system and experimental conditions:
- Experiment 1: Standard plate test (SPT)
Test tubes containing 2 ml soft agar kept in a water bath at 45°C, and remaining components added in the following order:
0 .1 ml test solution or vehicle
0 .1 ml bacterial suspension
0 .5 ml S-9 mix (in tests with metabolic activation) or 0 .5 ml phosphate buffer (in tests without metabolic activation).
After mixing, the samples are poured onto Vogel-Bonner agar plates.
Experiment 2: Preincubation assay (PT)
0.1 ml test solution or vehicle, 0.1 ml bacterial suspension and 0 .5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes. Subsequently, 2 ml of soft agar is added and, after mixing, the samples are poured onto the Vogel-Bonner agar plates.
Experiment1 & 2:
In each experiment 3 test plates per dose or per control used; after incubation at 37°C for 48 hours in the dark, the bacterial colonies ( his+/trp+ revertants) are counted.
Positive control:
with metabolic activation: 2.5 (Salmonella) or 60 (E. coli) μg/plate 2-aminoanthracene for all strains;
without metabolic activation: 5 μg/plate N-methyl-N'-nitro-N-nitrosoguanidine for TA 100 and TA 1535, 10 μg/plate 4-nitro-o-phenylendiamine for TA 98, 100 μg/plate 9-aminoacridine for TA 1537 and 5 µg/plate 4-nitroquinoline-N-oxide for E. Coli WP2 uvrA, all substances were dissolved in DMSO.
The titer was determined and in regularly measurements the strain characteristics were checked. Sterility control was performed. - Evaluation criteria:
- The test chemical is considered positive in this assay if the following criteria are met:
A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system. - Statistics:
- not performed
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA 1535, TA 100, TA 1537, TA 98; E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: >=2500 µg/plate in both the SPT and PIT; depending on strain and test condition
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Standard plate test (20 - 5000 µg/plate) | |||||
Strain | Metabolic activation system | mean revertants in Controls | maximum revertant factor | dose dependency | Assessment |
TA 98 | no | 33 | 0.9 | no | negative |
yes | 44 | 0.9 | no | negative | |
TA 100 | no | 103 | 1.1 | no | negative |
yes | 114 | 1.2 | no | negative | |
TA 1535 | no | 18 | 1.1 | no | negative |
yes | 18 | 1.0 | no | negative | |
TA 1537 | no | 9 | 1.4 | no | negative |
yes | 11 | 1.1 | no | negative | |
WP2 uvr A | no | 37 | 1.1 | no | negative |
yes | 37 | 1.1 | no | negative | |
Preincubation test (20 - 5000 µg/plate) | |||||
Strain | Metabolic activation system | mean revertants in Controls | maximum revertant factor | dose dependency | Assessment |
TA 98 | no | 26 | 1.0 | no | negative |
yes | 31 | 0.9 | no | negative | |
TA 100 | no | 110 | 1.0 | no | negative |
yes | 108 | 1.0 | no | negative | |
TA 1535 | no | 17 | 1.0 | no | negative |
yes | 17 | 0.9 | no | negative | |
TA 1537 | no | 10 | 1.1 | no | negative |
yes | 10 | 0.9 | no | negative | |
WP2 uvr A | no | 31 | 1.0 | no | negative |
yes | 36 | 1.0 | no | negative |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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