Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 939-725-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- other information
- Study period:
- 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Referenced sources in an international report.
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Dimethylformamide, SIDS Initial Assessment Report For SIAM 13
- Author:
- OECD
- Year:
- 2 001
- Bibliographic source:
- UNEP PUBLICATIONS
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: to Ulrich, P. et al.: Toxicology 125, 149-168
- GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- N,N-dimethylformamide
- EC Number:
- 200-679-5
- EC Name:
- N,N-dimethylformamide
- Cas Number:
- 68-12-2
- Molecular formula:
- C3H7NO
- IUPAC Name:
- N,N-dimethylformamide
- Test material form:
- not specified
- Details on test material:
- No further data
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- female
- Details on test animals and environmental conditions:
- Age: 6-8 weeks old
Study design: in vivo (non-LLNA)
- Positive control substance(s):
- not specified
Study design: in vivo (LLNA)
- Vehicle:
- other: DAE433, DMSO, DMF and acetone /oil olive (AOO)
- Concentration:
- Not reported
- No. of animals per dose:
- 6 animals
- Details on study design:
- N,N-dimethylformamide was used as a vehicle in the present investigation. The publication describes the validation of a two-tiered murine local lymph node assay. Groups of 6 female BALB/C strain mice (6-8 weeks old) were used. During tier I a wide range of concentrations of test chemical solutions or vehicle (volume: 25 μl) were applied on three consecutive days to the dorsum of both ears. Mice were killed 24 hours after the last application to determine ear and local lymph node weights and lymph node cell counts. Ear weights were determined to correlate chemical induced skin irritation with the eardraining lymph node activation potential. For comparison of the induction and challenge responses, mice were treated on the shaved back with 50 μl of test chemical or vehicle alone on three consecutive days (induction phase treatment). Then mice were challenged 12 days after the final induction phase exposure with 25 μl of test chemical or vehicle on the dorsum of both ears for a further 3 days (challenge phase treatment). Lymph nodes were excised 24 hours after the final challenge phase treatment. A tier II LLNA protocol was used to finally differentiate between true irritants and contact allergens.
To investigate the impact of different vehicles on the primary response induced by two contact allergens, i.e dinitrochlorobenzene (DNCB) at 0.5% and by eugenol at 35%, DAE433, DMSO, DMF and acetone /oil olive (AOO) were used. Both contact allergens were compared either to the untreated control (aqua bidest) or to the corresponding vehicle control.
Results and discussion
Any other information on results incl. tables
Topical treatment of mice with the vehicle N,N-dimethylformamide led to slight ear-draining lymph node activation as expressed by increased weights and cell counts in comparison to the untreated animals. However, this observation was not reproducible in a second experiment (i.e. when DMF was tested as vehicle for eugenol and as vehicle alone in comparison to the respective untreated control group).
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Topical treatment of mice with the vehicle N,N-dimethylformamide led to slight ear-draining lymph node activation as expressed by increased weights and cell counts in comparison to the untreated animals. However, this observation was not reproducible in a second experiment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.