Registration Dossier
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EC number: 248-211-9 | CAS number: 27076-30-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study witout GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-[(2-hydroxyethyl)amino]propionamide
- EC Number:
- 248-211-9
- EC Name:
- 3-[(2-hydroxyethyl)amino]propionamide
- Cas Number:
- 27076-30-8
- Molecular formula:
- C5H12N2O2
- IUPAC Name:
- N~3~-(2-hydroxyethyl)-beta-alaninamide
- Test material form:
- other: colourless slightly viscous liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Source: The aeration stage of Severn Trent Water PLC Sewage
Treatment Plant, Belper, Derbyshire.
Preparation: The activated sludge was washed by repeatedly settling and resuspending with the test water.
Usage rate: Equivalent to 750 mg dry weight/L .
Standard Material:Diethylene glycol - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 327 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Losses by evaporation were made good by the addition of de-ionised water immediately prior to sampling.
20 ml samples were withdrawn from each vessel and filtered through 0.45 um disposable filters, the first 5 ml was discarded.
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 81
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 91% after 8 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- ACMEO attained 8l% degradation after 28 days and so, therefore, has shown evidence of
ultimate biodegradabiIity. - Executive summary:
ACMEO attained 8l% degradation after 28 days and so, therefore, has shown evidence of ultimate biodegradability. The Standard Material, Digol, attained 99% degradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions. pH values did not drop below 5.1 in any culture vessel during the course of the study and were adjusted to pH 7.3 daily. Dissolved oxygen levels remained at approximately 6.3 mg O2/L or above in all cultures at all times.
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