Guanidine, N-phenyl-, compd. with carbonic acid, hydrate (2:1:2)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July - December 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Remarks:

This study has been performed in compliance with GLP in Switzerland, Procedures and Pronciples, March 1986, issued by the Swiss federal Department of the Interior and the Intercantonal Office for the Control of Medicaments.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

155 mg CaCO3/l.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: none

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: none

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: none

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: none

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortalities (Initial number of fishes = 10/test conc.)

 

Conc. nominal mg/l	Mortalities Number of dead fish
	24 h	48 h	72 h	96 h
Blank	0	0	0	0
10	0	0	0	0
18	0	0	0	0
32	0	0	0	0
58	0	0	0	0
100	0	0	0	0

 

Symptoms observed   

Conc. nominal mg/l	Swimming behaviour				Loss of equilibrium				Respiratory function				Exophtalmus				Pigmentation
	24	48	72	96	24	48	72	96	24	48	72	96	24	48	72	96	24	48	72	96 h
Blank	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
18	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
32	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
58	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

 0 = no symptoms

Validity criteria fulfilled:

yes

Remarks:

Criteria: mortality in blank < 10%, constant conditions throughout the test, dissolved oxygen concentration > 60 % of the air saturation value, stable concentration of test substance throughout the test

Conclusions:

LC50,96h calculated: > 100 mg/l
NOEC96h: 100 mg/l
LC0,96h: 100 mg/l
LC100,96h: > 100 mg/l

Executive summary:

This study was performed in order to determine the acute toxicity of CA 1139 A to Rainbow Trout (Oncorhynchus mykiss).

The test was conducted according the OECD guideline 203.

10 fishes per concentration and control were used. Each aquarium was occupied by 5 fishes.

The nominal test concentrations were: 10, 18, 32, 58 and 100 mg test substance/l.

Mortalities were recorded after 24, 48, 72 and 96h exposure.

The fishes were examined daily for the symptoms.

The observed results were as follows:

LC50,96h calculated: > 100 mg/l

NOEC96h: 100 mg/l

LC0,96h: 100 mg/l

LC100,96h: > 100 mg/l

Values are based on nominal concentrations.

Description of key information

This study was performed in order to determine the acute toxicity of CA 1139 A to Rainbow Trout

(Oncorhynchus mykiss).
The test was conducted according the OECD guideline 203.
10 fishes per concentration and control were used. Each aquarium was occupied by 5 fishes.
The nominal test concentrations were: 10, 18, 32, 58 and 100 mg test substance/l.
Mortalities were recorded after 24, 48, 72 and 96h exposure.
The fishes were examined daily for the symptoms.
The observed results were as follows:
LC50,96h calculated: > 100 mg/l
NOEC96h: 100 mg/l
LC0,96h: 100 mg/l
LC100,96h: > 100 mg/l
Values are based on nominal concentrations.

Additional information

 

 

Guideline Test Type	Species	Conclusion	Reference	Reliability
OECD 203	Oncorhynchus mykiss	LC50(96 h) > 100 mg/l NOEC (96 h): 100 mg/l LC0(96 h): 100 mg/l	Grade R., Ciba-Geigy, 1992	Reliable without restrictions