Registration Dossier
Registration Dossier
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EC number: 248-479-7 | CAS number: 27472-21-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 05 to 07 May 2010
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Unsuitable test system and documentation is insufficient for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (3,4-diethoxyphenyl)acetonitrile
- EC Number:
- 248-479-7
- EC Name:
- (3,4-diethoxyphenyl)acetonitrile
- Cas Number:
- 27472-21-5
- Molecular formula:
- C12H15NO2
- IUPAC Name:
- 2-(3,4-diethoxyphenyl)acetonitrile
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- other: hisD3052 rfa uvrB +R
- Species / strain / cell type:
- other: Salmonella his- TA7001 - 7006
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 0, 16, 50, 160, 500, 1600 and 5000 µg/mL
Controls
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- other: 2-Aminoanthracene
- Remarks:
- -S9-Mix: 625/1200 ng/ml 4-nitroquinoline N-oxide/2-nitrofluorene +S9-Mix: 10 µg/ml 2-aminoanthracene
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: Salmonella his- TA7001 - 7006
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the experimental conditions of the study, the substance is considered to be not mutagenic with and without metabolic activation in a bacterial reverse mutation test conducted on the Salmonella typhimurium TA98 and mixed strains (TA7001 - 7006), testing up to 5000 µg/mL.
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