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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
1967-06
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study is classified as reliable with restriction because although the study was not GLP compliant, it was conducted according to FHSLA recommendations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Federal Hazardous Substances Labeling Act
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Hexadec-1-ene
EC Number:
211-105-8
EC Name:
Hexadec-1-ene
Cas Number:
629-73-2
IUPAC Name:
hexadec-1-ene
Details on test material:
- Name of test material (as cited in study report): Alpha olefins (C16)
- Substance type: C16 alpha olefin
- Physical state: Liquid
-Specific gravity at 20 degrees Celsius: 0.7817

Test animals / tissue source

Species:
other: albino rabbit

Test system

Vehicle:
not specified
Controls:
other: The non-treated eye of each animal was used as a control.
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.01 millilitre
Duration of treatment / exposure:
One application/exposure
Observation period (in vivo):
Animals were observed 24, 48 and 72 hours after exposure.
Number of animals or in vitro replicates:
0.01 ml of the test material was instilled into one eye of six male rabbits.
Details on study design:
SCORING SYSTEM: The Draize scoring system was used.

Results and discussion

In vivo

Results
Irritation parameter:
other: Draize
Basis:
mean
Time point:
other: 24 hour
Score:
0.7
Remarks on result:
other: Not irritating
Irritant / corrosive response data:
Instillation of 1-hexadecene in the eyes produced mild, transient redness of the conjunctival tissue. No effects on the cornea or iris were observed. Based on this test result, the author concluded that 1-hexadecene is not an eye irritant to albino rabbits.
Other effects:
The eye treated with control substance, 5% Ivory solution, showed greater response than any of the test substances.

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating according to EU CLP classification
Remarks:
Criteria used for interpretation of results: EU
Conclusions:
Instillation of 1-hexadecene in the eyes produced mild, transient redness of the conjunctival tissue. No effects on the cornea or iris were observed. Based on this test result, the author concluded that 1-hexadecene is not an eye irritant to albino rabbits.
Executive summary:

In a primary eye irritation study, 0.1 millilitre of 1-hexadecene was instilled into the conjunctival sac of six male albino rabbits. Treated eyes were not washed during exposure. The animals were observed at 24, 48 and 72 hours after exposure. Irritation was scored by the Draize method. In this study, 1-hexadecene is not an eye irritant based on the 24 hour average redness Draize score of 0.7.

This study received a Klimisch score of 2 and is classified as reliable with restrictions because although the study was not GLP compliant, it was conducted according to FHSLA recommendations.