1,6-hexanediyl-bis(2-(2-(1-ethylpentyl)-3-oxazolidinyl)ethyl)carbamate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1990-09-12 to 1990-10-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

according to guideline

Guideline:

other: Biologische Abbaubarkeit - Respirometer test. The test was performed in accordance with regulation (EC) 79/831 EWG Annex V. Modifications were done in accordance with EEC Directive 79/831 (Painter).

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: 10 L laboratory (Wupperverband)
- Sampling date: 12.09.1990
- Pretreatment: no

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Procedure:
The test item was dissolved and suspendeed, respectively, in non-mineralic medium. Afterwards non-adapted activated sludge was added. After 28 days exposure (contact time) the oxygen consumption was measured in a closed respirometer (at a temperature of 20 ± 1 °C) automatically. The biological degradation was determined as the oxygen consumption (%) based on the theoretical oxygen demand (TOD) and CSB (corrected by the oxygen demand of the blank), respectively.

Others:
- Amount of substance: 100 mg/L
- TOD (Theoretical oxygen demand): 2646 mg/L
- Concentration of inoculum: 30 mg/L

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

43

Sampling time:

28 d

Details on results:

Time (days) / oxygen consumption mg/L of test item; blank / biodegradation (%)
- 2 / 10; 7 / 1
- 6 /93; 14 / 30
- 8 / 110; 18 / 35
- 12 / 129; 22 / 40
- 14 / 133; 23 / 42
- 16 / 136; 24 / 42
- 20 / 142; 29 / 43
- 22 / 145; 32 / 43
- 26 / 147; 33 / 43
- 28 / 148; 33 / 43

The theoretical oxygen demand of NO3- was used as reference for the calculation of the biodegradation.

Results with reference substance:

Aniline showed 61 % biodegradation after 28 days.

Based on the results with the reference substance the study result was considered valid.

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable, fulfilling specific criteria

Conclusions:

The test item was biodegraded 43 % at day 28 in the test using a closed respirometer. The test item was considered inherently biodegradable.

Executive summary:

A ready biodegradability test with the test item was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined using a closed respirometer according to test method outlined in EEC Directive 79/831. The test item was biodegraded 43 % at day 28. Therefore, the test substance was considered inherently biodegradable.

Description of key information

The test item was biodegraded 43 % at day 28 in the test using a closed respirometer. The test item was considered to be inherently biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable, fulfilling specific criteria

Type of water:

freshwater

Additional information

A ready biodegradability test with the test item was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined using a closed respirometer according to test method outlined in EEC Directive 79/831. The test item was biodegraded 43 % at day 28. Therefore, the test substance was considered to be inherently biodegradable.