Registration Dossier
Registration Dossier
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EC number: 412-420-5 | CAS number: 133909-99-6
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Bacteriologcal test (Suspension of bacterial cells) with metabolic activation / without metabolic activation
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
Method
- Target gene:
- Bacterial cells
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Supplemented liver fraction (S-9 mix) prepared from an animal previously treated with Aroclor 1254
- Test concentrations with justification for top dose:
- Concentration range in the main test (µg/plate)
with metabolic activation : 50 5000
without metabolic activation : 50 5000
Concentration of test substance observed to be toxic to bacteria (µg/plate) :
a) in a preliminary test with metabolic activation >5000
without metabolic activation > 5000
b) in the main test with metabolic activation >5000
without metabolic activation > 5000
Concentration of the test substance resulting in precipitation : 1500 µg/plate
Controls
- Untreated negative controls:
- yes
- Remarks:
- Dimethyl sulfoxide
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl sulfoxide
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
With metabolic activation : negative
Without metabolic activation : negative
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
