1,8-naphthylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

(1992)

Deviations:

no

Principles of method if other than guideline:

At defined concentrations of a test substance arise after the testing period, different percentages of dead fish. The LC 100 and LC 0-values ​​are determined directly from the analysis, while the LC 50 value is determined by calculation or graphically from the concentration-response curve.
The test duration is 96 hours.

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

- Concentration: Concentration of 1 mg/L and concentration of 0 mg/L (control) were measured at 0, 24, 48, 72 and 96 hours; and a concentration of 3.16 mg/L was measured at 0 and 24 hours.
- The samples were not analysed monitored.

Vehicle:

no

Details on test solutions:

- The weighed substance in water (1 and 3.16 mg / L) was used to prepare the individual assays for 1 hour in an ultrasonic bath and stirred for 24 hours on a magnetic stirrer.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Name: Zebra fish (Danio rerio)
- Source: Bio International B.V. {Niederlande)
- Date supplied: 1997-04-08
- Temperature: 21.0 - 22.5 °C
- Dissolved oxygen: 7.4 - 9.2 mg/L
- Mortalities during acclimatisation period: < 2 %
- Medication: none
- Mean standard lenght: 2.5 - 3.5 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

no data

Test temperature:

21.7 - 22.5 °C measured at each test vessel at the beginning and the end of the test
21.0 - 22.4 °C measured at each test vessel at the beginning and the end of the control

pH:

7.5 - 8.0 measured at each test vessel at the beginning and the end of the test
7.6 - 8.2 measured at each test vessel at the beginning and the end of the control

Dissolved oxygen:

8.1 - 9.2 mg O2/L (with 94.4 - 108.0 % saturation) measured at each test vessel at the beginning and the end of the test
7.4 - 8.5 mg O2/L (with 85.3 - 100.4 % saruration) measured at each test vessel at the beginning and the end of the control

Nominal and measured concentrations:

Test concentrations: 1 and 3.16 mg/L plus control

Details on test conditions:

- Test vessles: glass aquaria holding 5 L of the test media
- Experimental design: 2 test concentrations plus 1 control, 10 fish per test concentration, ventilated, static
- Photoperiod: 16 h light : 8 h dark
- Temperature: 21.0- 22.5 °C
- Test item concentrations: 1 and 3.16 mg/L
- Method of administration: direct weighing
- Duration of exposure: 96 hours
- Criteria of effects: The criteria of death used in this study was the absence of response to physical stimulation. In addition to observations on mortality at 24, 48, 72 and 96 hours, type and incidence of sub lethal effects compared with control fish were observed.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

3.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: LC 0/LC 100 (geometric mean)

Effect conc.:

1.78 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity to fish (danio rerio) was tested in a staic test. After 96 hours of exposure a LC0 of 1 mg/L and a LC100 of 3.16 mg/L were determined. The geometric mean at LC0 / LC100 was calculated to be 1.78 mg/L.

Executive summary:

A study was performed to assess the acute toxicity to fish (danio rerio) of the substance under static conditions. The study was conducted in accordance with council directive 67/548/EWG, method C.1 'acute toxicity to Fish' (1992). After 96 hours of exposure a LC0 of 1 mg/L and a LC100 of 3.16 mg/L was determined. The geometric mean at LC0 / LC100 was calculated to be 1.78 mg/L.

Description of key information

The acute toxicity to fish (danio rerio) was tested in a staic test. After 96 hours of exposure a LC0 of 1 mg/L and a LC100 of 3.16 mg/L were determined. The geometric mean at LC0 / LC100 was calculated to be 1.78 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.78 mg/L