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EC number: 700-292-6 | CAS number: 135043-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Follows a recognised guideline and performed to GLP standards.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
- IUPAC Name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Mixed population of activated sewage sludge micro-organisms obtained from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, UK, which predominantly treats domestic sludge.
- Preparation of inoculum for exposure: washed three times by settlement and resuspension in culture medium to remove any excessive amounts of dissolved organic carbon (DOC) that may be present. The washed sample was then maintained on continuous aeration in the laboratory at a temperature of approximately 21 C and used on the day of collection.
- Suspended solids concentration: 1.9 g/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Solution A: KH2PO4 8.50 g/l, K2HPO4 21.75 g/l, Na2HPO4.2H2O 33.40 g/l. NH4Cl 0.50 g/l; Solution B: CaCl2 27.50 g/l; Solution C: MgSO4.7H2O; Solution D: FeCl3.6H2O 0.25 g/l.
- Test temperature: 21 C
- pH: 7.4
- pH adjusted: no
- Suspended solids concentration: 30 mg (ss)/l
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5 litre culture vessels.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: CO2-free air bubbled through the solution at a rate of approximately 40 ml/minute stirred continuously by magnetic stirrer.
- Measuring equipment: CO2 analyser - Tekmar-Dohrmann Apollo 9000 TOC analyser and a Shimadzu TOC-V(CSH) TOC analyser. DOC analyser - Shimadzu TOC-5050A TOC analyser.
- Details of trap for CO2 and volatile organics if used: CO2 produced by degradation was collected in 2 500ml Dreschel bottles containing 350 ml of 0.05 M NaOH.
SAMPLING
- Sampling frequency: Samples were taken from the first CO2 absorber vessel on days 0, 1, 2, 3, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 27, 28 and 29. The second absorber vessel was sampled on days 0 and 29.
- Other: CO2 analysis: The samples taken on day 0, 1, 2, 3, 6, 8, 10, 14, 16, 20, 24, 28 and 29 were analysed for CO2 immediately. The samples taken on days 12, 18, 22 and 27 were stored at approximately -20 C. On day 28, 1 ml of concentrated HCl was added to each absorber vessel to drive off any inorganic carbonates formed. These vessels were resealed, aerated overnight and the final samples taken from absorber vessels on day 29. DOC analysis: On day 0 and 28, samples were removed from all culture vessels and filtered through filters prior to DOC analysis.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- Results show that trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate did not absorb to filter matrices or to activated sewage sludge. Therefore samples taken for DOC analysis were filtered to remove the suspended solids present without the loss of any test material.
- Test performance:
- The total CO2 evolution in the control vessels on day 28 was 15.70 mg/l and the IC content of the test material suspension in the mineral medium at the start of the test was below 5% of the TC content; both satisfied the validation criterion given in the OECD test guidelines.
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 108
- Sampling time:
- 29 d
- Details on results:
- Results of the main study are in table 1. Trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate attained 108% degradation after 29 days and satisfied the 10-day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation exceeding 10%.
The toxicity control attained 96% degradation after 14 days and 106% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions. Analysis of the test media from the test material culture vessels on day 0 and 28 for DOC can be seen in table 2.
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate attained 103% degradation after 14 days and 105% degradation after 28 days thereby confirming the suitability of the inoculum and test conditions (see table 1).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate attained 108% degradation after 29 days and satisfied the 10-day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation exceeding 10%. Therefore trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate can be considered to be readily biodegradable under the conditions of OECD guideline 301B.
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