Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-728-6 | CAS number: 99-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- NEN 6502: Determination of chronic toxicity to Daphnia magna (1980)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 3-Nitrotoluene
- Analytical purity: > 98% - Details on test solutions:
- synthetic test medium (NPR, 1980)
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- < 24 h old
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
- Hardness:
- 200 mg/l as CaCO3
- Test temperature:
- 20 ± 0.5
- pH:
- 8.4 ± 0.1
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.8 mg/L
- Basis for effect:
- growth
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.21 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 8.3 mg/L
- Executive summary:
Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). All daphnids used were < 24 h old at the start of the experiments. The study reports the following effect concentrations:
log (LC50): 1.78 corresponds to 60.255 µmol/L; 8.3 mg/L
log (LOEC reproduction): 1.37 corresponds to 23.44 µmol/L; 3.21 mg/L
log (LOEC growth): 1.12 corresponds to 13.18 µmol/L; 1.8 mg/L
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- in analogy with the OECD 202 proposal 1979.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 3-nitrotoluene
- Analytical purity: 99.5 % - Analytical monitoring:
- yes
- Details on test solutions:
- - Culturing and test medium: NaHCO3 100 mg/l, CaCl2*2H2O 200 mg/l, KHCO3 20 mg/l, MgSO4*7H2O 180 mg/l
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Daphnias were 1 day old at start of incubation
- Food during incubation: Chlorella
- 25 organisms per 1 litre of test medium, 2 replicates - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- - Incubation temperature 19 ± 1 °C
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 3.5 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.5 mg/L
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 9 mg/L
- Basis for effect:
- other: mortality + behaviour
- Executive summary:
The toxicity of 3-nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.
Referenceopen allclose all
Two additional chronic effects presented:
1. Population growth:
LRCT(Rm) = Lowest rejected concentration (µmol/l) tested that significantly (p<0.01) lowered the population growth constant (Rm) after 21 days of exposure.
log LRCT(Rm) = 1.37
2. Body length:
LRCT(L) = Lowest rejected concentration (µmol/l
tested that significantly (p< 0.01) lowered the mean length (L) of animals after 21 days of exposure.
log LRCT(L) = 1.12
Description of key information
For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".
The toxicity of 3-nitrotoluene to the aquatic invertebrate Daphnia magna was evaluated in a 21 d study performed in analogy with the OECD 202 proposal (1979). The 21d-EC50 for reproduction was determined to be 3.5 mg/L, while the same endpoint for mortality and behaviour was 9.0 mg/L (Canton et al. 1985). Additionally, a NOEC value of 0.5 mg/L was accounted in the same study.
Deneer et al. (1989) carried out a study on Daphnia magna, in semi-static test conditions, following the protocol of the Dutch Standards Organization NEN 6502 (1980). All daphnids used were < 24 h old at the start of the experiments. The study reports the following effect concentrations:
log (LC50): 1.78 corresponds to 60.255 µmol/L; 8.3 mg/L
log (LOEC reproduction): 1.37 corresponds to 23.44 µmol/L; 3.21 mg/L
log (LOEC growth): 1.12 corresponds to 13.18 µmol/L; 1.8 mg/L
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.