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EC number: 939-600-0 | CAS number: 1471316-27-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro genotoxicity studies were performed with Alcohols, C16-18 (even numbered) and C18 unsaturated, propoxylated, < 2.5 PO (CAS 70955-07-6).
Mutagenicity in bacteria
Mutagenicity in bacteria was assessed in a study performed with Alcohols, C16-18 (even numbered) and C18 unsaturated, propoxylated, < 2.5 PO (CAS 70955-07-6) according to OECD Guideline 471 and under GLP conditions (Woitkowiak, 2012). Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100, and E.coli strain WP2 were treated using the plate incorporation method as well as the preincubation method, both with and without the addition of a rat liver S9-mix. Concentrations tested were 33, 100, 333, 1000, 2500 and 5000 µg/plate. All tests were done in triplicates. The vehicle (acetone) and negative (untreated) control plates produced counts of revertant colonies within an acceptable range. All positive controls used in the test induced marked increases in the frequency of revertant colonies, both with and without the metabolising system. A reproducible mutagenic activity of the test material to any of the tester strains was not observed with and without metabolic metabolic activation. Precipitation of the test substance was observed from 2500μg/plate onward with and without S9 mix.
Thus, under the conditions of this test the test substance can be regarded as not mutagenic in bacteria.
Mutagenicity in mammalian cells
The mutagenic potential in mammalian cells was assessed with Alcohols, C16-18 (even numbered) and C18 unsaturated, propoxylated, < 2.5 PO (CAS 70955-07-6) by a HPRT-assay according to OECD Guideline 476 and under GLP conditions (Wolny, 2013).In the first experiment Chinese hamster lung fibroblasts (V79) were exposed for 4 h to 2.5, 5.0, 10.0, 20.0, 40.0 and 60.0 µg/mL test substance in the absence and 2.5, 5.0, 10.0, 20.0, 40.0, 80.0, and 120 µg/mL test substance in the presence of metabolic activation. In the second experiment the cells were exposed for 24 h (without metabolic activation) and 4 h (with metabolic activation) to 2.5, 5.0, 10.0, 20.0, 40.0 and 60.0 µg/mL test substance. Positive and vehicle (ethanol) control cultures were included in each assay. Cytotoxicity was observed in the first (4 h) and the second experiment (24 h) at concentrations at 40 µg/mL without metabolic activation. No increased number of chromosome aberrations in the presence or absence of metabolic activation was observed at any concentration tested. Appropriate reference mutagens used as positive controls showed a significant increase in chromosome aberrations, thus indicating the sensitivity of the assay, and the efficacy of the S9-mix. Hence, the test substance is not clastogenic.
Genotoxicity in mammalian cells
The clastogenic potential was assessed in a chromosomal aberration test with Alcohols C16-18 (even numbered) and C18 unsaturated, propoxylated, < 2.5 PO (CAS 70955-07-6) in mammalian cells according to OECD Guideline 473in five independent experiments where two parallel cultures were set up, according to the following scheme:
|
Without S9 mix |
With S9 mix |
|||
Exp. IA |
Exp.IIA |
Exp. IB and IC |
Exp. IIA and IIB |
||
Exposure period |
4h |
18 h |
4 h |
4h |
|
Recovery |
14 h |
-- |
-- |
14 h |
24 h |
Preparation interval |
18 h |
18 h |
28 h |
18 h |
28 h |
100 metaphases per culture were evaluated for structural chromosome aberrations. For the positive controls in Experiment IIA and IIB after 28 hours preparation interval with metabolic, only 50 metaphases were evaluated.
The highest applied concentration (5.2µL/mL) was chosen with regard to the purity (96.6%) of the test item and the recommended top dose.
The concentration selection for the cytogenicity experiments was performed considering the toxicity data of a pre-experiment.
In experiment IA in the absence of metabolic activation and in Experiment IC in the presence of metabolic activation, cytotoxicity indicated as reduced mitotic index was observed at the highest evaluated concentration (49.7, 45. 3% of control, respectively).
No clastogenicity was observed at concentrations evaluated either with or without metabolic activation. No relevant increase in polyploid metaphases was observed after treatment with the test item in comparison with the control cultures.
Positive controls (EMS and CPA) induced significant increases with structural chromosome aberrations.
In conclusion, under the experimental conditions of the study, the test item did not induce structural chromosome aberrations in V79 cells in vitro.
Summary
Alcohols, C16-18 (even numbered) and C18 unsaturated, propoxylated, < 2.5 PO (CAS 70955-07-6) is regarded as non-genotoxic.
Justification for selection of genetic toxicity endpoint
No study selected as all results are negative.
Short description of key information:
Ames (OECD 471): not mutagenic in bacteria
Chromosomal Aberration (OECD 473): not clastogenic in mammalian cells
HPRT (OECD 476): not mutagenic in mammalian cells
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
According to the classification criteria of Directive 67/548/EEC
and Regulation (EC) No 1272/2008 the substance does not need to be
classified for genotoxicity.
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