Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 July 1987 to 20 July 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: EPA Guideline 123-2
- Deviations:
- yes
- Remarks:
- See principles of method if other than guideline
- Principles of method if other than guideline:
- According to the protocol the light intensity for the 5-day toxicity test with Anabaena flos-aquae should be 2000 lux. Cell biomass of Anabaena flos-aquae increased slowly when cultured under this low light intensity. Therefore, the light intensity was increased during culturing and testing of this species to 5000 and 4500 lux, respectively. It is the opinion of the testing laboratory that this did not affect the results of the study.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Prepared every 3 days as a stock solution by adding test material to M-Type Hoaglands medium. The stock was ultrasonicated (for 10 minutes) to ensure that the test material dissolved. - Test organisms (species):
- Lemna gibba
- Details on test organisms:
- TEST ORGANISM
- Common name: duckweed
- Strain: Lemna gibba G3 - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC10
- Effect conc.:
- 19 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 33 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, the 14 day EC50 for inhibition of growth (frond production) of Lemna gibba G3 was determined to be 33 µg/L (95 % C.I. 15-75 µg/L).
- Executive summary:
In a GLP compliant effects on aquatic plants study conducted in line with standardised guideline EPA Guideline 123 -2, the effects of the test material on aquatic plants was determined. Aquatic plants were exposed to the test material at nominal test concentrations of 0, 6.3, 13, 25, 50 and 100 µg/L. Under the conditions of the test, the 14 day EC50 for inhibition of growth (frond production) was determined to be 33 µg/L.
Reference
The results of this test are detailed in Table 1 below.
Table 1: Mean frond production
Days of exposure | |||||
Exposure concentration (µg/L) | 3 | 6 | 9 | 12 | 14 |
Controls | 43 | 103 | 211 | 358 | 463h |
6.3 | 43 | 103 | 223g | 399 | 517h |
13 | 36 | 114f | 337g | 634f | 875f |
25 | 24a | 41f | 96f | 154i | 266i |
50 | 20b,c | 18d | 23e | 22e,j | 23e |
100 | 16 | 15d | 15e | 16e | 16e |
EC50 | 48 | 30 | 36 | 33 | 33 |
aAll fronds slightly yellow; bAll fronds mostly brown; cMany small individual fronds; dSome fronds brown, individual; eFronds brown, individual; fFronds small, some are yellow; gFronds smaller than controls; hFew yellow fronds; iFew fronds same size as controls, most are very small, few roots observed; jRoots are brown
The EC10, EC50 and EC90 values and their 95% confidence limits were determined by linear regression of the response (percent reduction of frond production, as compared with controls) versus nominal exposure concentration over the range of test concentrations where a clear exposure-response relationship was observed. They are presented in Table 2 below.
Table 2: EC10, EC50 and EC90 values
Exposure duration | EC10 (µg/L) (95% C.I.) | EC50 (µg/L) (95% C.I.) | EC90 (µg/L) (95% C.I.) |
3 days | 8.6 (4.6 -15) | 48 (27 -87) | 270 (140 -550) |
6 days | 10 (3.0 -31) | 30 (9.7 -95) | 85 (28 -310) |
9 days | 19 (7.4 -47) | 36 (14 -92) | 71 (29 -200) |
12 days | 18 (7.2 -44) | 33 (14 -82) | 63 (26 -170) |
14 days | 19 (8.2 -41) | 33 (15 -75) | 63 (29 -150) |
Description of key information
The EC50 of the test material was determined to be 33 µg/L according to a study performed in line with EPA Guideline 123-2.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 33 µg/L
- EC10 or NOEC for freshwater plants:
- 19 µg/L
Additional information
In a GLP compliant effects on aquatic plants study conducted in line with standardised guideline EPA Guideline 123-2, the effects of the test material on aquatic plants was determined. Aquatic plants were exposed to the test material at nominal test concentrations of 0, 6.3, 13, 25, 50 and 100 µg/L. Under the conditions of the test, the 14 day EC50 for inhibition of growth (frond production) was determined to be 33 µg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.