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EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 July 1987 to 01 August 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- 50 mL aliquots of test aquaria water were transferred to 70 mL culture tubes. 10 mL of toluene was added to each tube and shaken vigirously for three minutes. The phases were allowed to separate and the toluene phase was transferred to clean culture tubes. The samples were then diluted with toluene when necessary.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: in-house culture
- Feeding during test
- Food type: algal suspension (Selenastrum capricornutum) with additional suspension of Tetramin, cereal leaves, vitamin and yeast
- Frequency: three times daily - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 238-278 mg/L as CaCO3 (well water)
- Test temperature:
- 20 °C
- pH:
- 8.0-8.2
- Dissolved oxygen:
- 8.5-9.1 mg/L
- Nominal and measured concentrations:
- 0, 0.3, 0.6, 1.2, 2.5 and 5.0 mg/L (nominal)
0, 0.32, 0.56, 1.0, 2.3 and 4.3 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 litre glass beakers
- Type (delete if not applicable): closed
- Type of flow-through: proportional diluter
- Renewal rate of test solution (frequency/flow rate): 5.7 times in a 24 hour period
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ABC well water
- Total organic carbon: 0.6-3.8 mg/L
- Particulate matter: 0-1.3 mg/L
- Metals: <0.2 mg/L
- Chlorine: <0.05 mg/L
- Alkalinity: 300-320 mg/L (CaCO3)
- Conductivity: 480-540 µmhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hour light/8 hour dark
EFFECT PARAMETERS MEASURED: survival, abnormal effects and observance of first brood daily; length of surviving adults at study termination
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: 0.3, 0.6, 1.2, 2.5 and 5.0 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Key result
- Duration:
- 21 d
- Dose descriptor:
- other: Maximum Acceptable Toxicant Concentration
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, young/adult/reproduction day and adult mean length
- Details on results:
- An overview of the effects observed can be found in Table 1.
Daphnid survival was significantly different from the pooled controls at a mean measured concentration of 4.3 mg test material/L. The mean young/adult/reproduction day for 21 days were significantly affected in the mean measured exposure levels of 2.3 and 4.3 mg/L. No reproduction was observed in the 4.3 mg/L test concentration during this study. The average length in days to first brood for the controls was 8.5 days. The lengths to first brood were significantly affected in the mean measured exposure level of 1.0 and 4.3 mg/L. The effect identified for the 1.0 mg/L was considered aberrant since no effect was identified at the next higher test level. The adult daphnid lengths in the test material mean measured concentrations of 2.3 and 4.3 mg/L were significantly different from the pooled controls. - Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, the 21 day EC50 based on immobility was 3.4 mg/L. Based on the statistical analysis of survival, young/adult/reproduction day, and adult mean length, the MATC (Maximum Acceptable Toxicant Concentration) limits were estimated to be the mean measured concentration of > 1.0 and < 2.3 mg/L. The estimated MATC value was calculated from these data to be 1.5 mg/L.
- Executive summary:
In a GLP compliant chronic Daphnia toxicity study conducted in line with standardised guideline EPA OPP 72-4, the chronic toxicity to Daphnia of the test material over 21 days was determined. Daphnia were exposed to the test material at nominal test concentrations of 0, 0.30, 0.60, 1.2, 2.5 and 5 mg/L (equivalent to mean measured concentrations of 0.32, 0.56, 1.0, 2.3 and 4.3 mg/L). Under the conditions of the test, the 21 day EC50 based on immobility was 3.4 mg/L. Based on the statistical analysis of survival, young/adult/reproduction day, and adult mean length, the MATC (Maximum Acceptable Toxicant Concentration) limits were estimated to be the mean measured concentration of > 1.0 and < 2.3 mg/L. The estimated MATC value was calculated from these data to be 1.5 mg/L.
Reference
Table 1: Percent survival, young/adult reproduction day, length to first brood and adult length
Nominal conc. (mg/L) | Adult mean legth (mm) | Mean survival (%) | Mean young/adult reproduction day | Mean length to first brood (days) |
0 (control) | 3.8 | 95 | 5.4 | 8.5 |
0 (solvent control) | 3.8 | 95 | 5.4 | 8.5 |
0.30 | 3.8 | 95 | 5.3 | 8.5 |
0.60 | 3.9 | 92 | 5.4 | 8.2 |
1.2 | 3.9 | 95 | 5.2 | 101 |
2.5 | 3.4* | 95 | 3.6* | 9 |
5.0 | 2.1* | 18* | 0* | 21* |
*significanly different from pooled controls
1considered aberrant value
Description of key information
The EC50 of the test material, based on immobility, was determined to be 3.4 mg/L according to a study performed in line with EPA Guideline 72-4 (equivalent to NOEC of 1.06 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.06 mg/L
Additional information
In a GLP compliant chronic Daphnia toxicity study conducted in line with standardised guideline EPA OPP 72-4, the chronic toxicity to Daphnia of the test material over 21 days was determined.
Daphnia were exposed to the test material at nominal test concentrations of 0, 0.30, 0.60, 1.2, 2.5 and 5 mg/L (equivalent to mean measured concentrations of 0.32, 0.56, 1.0, 2.3 and 4.3 mg/L).
Under the conditions of the test, the 21 day EC50 based on immobility was 3.4 mg/L. Based on the statistical analysis of survival, young/adult/reproduction day, and adult mean length, the MATC (Maximum Acceptable Toxicant Concentration) limits were estimated to be the mean measured concentration of > 1.0 and < 2.3 mg/L. The estimated MATC value was calculated from these data to be 1.5 mg/L (equivalent to a NOEC of 1.06 mg/L in line with Table R.10-1 of 'Guidance on information requirements and chemical safety assessment Chapter R.10').
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