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EC number: 206-055-9 | CAS number: 298-06-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water and sediment: simulation tests
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study is a publication with detailed description of the method used.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- The degradation of sodium O,O-dietyl dithiophosphate by bacteria from metalworking fluids
- Author:
- Sherburn, R. E & Large, P. J.
- Year:
- 1 999
- Bibliographic source:
- Letters in Applied Microbiology, 28, 61-65
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Bacteria were isolated from contaminated metalworking fluids and identified using standard biochemical tests. Bacteria were cultured with the test substance NaDDP (sodium O,O-diethyl phosphorodithioate) and analysed for the formation of degradation products.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Sodium O,O-diethyl dithiophosphate
- EC Number:
- 222-079-2
- EC Name:
- Sodium O,O-diethyl dithiophosphate
- Cas Number:
- 3338-24-7
- IUPAC Name:
- sodium O,O-diethyl dithiophosphate
- Details on test material:
- - Name of test material (as cited in study report): Sodium O,O-diethyl dithiophosphate (O,O-diethyl phosphorodithioate; NaDDP)
Constituent 1
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- not specified
- Inoculum or test system:
- other: bacterial growth medium
- Duration of test (contact time):
- 120 min
Initial test substance concentration
- Initial conc.:
- 50 mmol/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: Production of degradation products
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
per 1 L of solution: 0.4 g MgSO4*7H2O, 7 g (NH4)SO4, 25 mg FeSO4*7H2O, 4.1 mg MnSO4*4H2O, 4.4 mg ZnSO4*7H2O, 0.79 mg CuSO4*5H2O, 73.4 mg CaCl2*2H2O, 80 mmol/L potassium sodium phosphate buffer (pH 7) and 50 mmol/L glycerol
TEST SYSTEM
ethanol was measured colorimemetrically with alcohol oxidase;
aldehydes were measured by the MBTH method;
phosphate was measured by the method of Lowry and Lopez;
hydrogen sulphide was measured by the method of Trüper and Schlegel;
acid phosphorodiesterase activity was measured by incubating extract in 2.2 mmol/L sodium citrate buffer (pH 6) with 0.1 mmol/L 2'-deoxythymidine-3'-(4-nitro-phenyl)phosphate for 5 min and stopping the reaction by adding 2.9 mL of 0.1 mol/L NaOH, absorbance was then measured at 405 nm;
alkaline phosphodiesterase activity was measured by incubating extract in 0.2 mol/L Tris-HCl (pH 8.9) containing 3.3 mg bis-4-nitrophenyl phosphate/mL, absorbance was determined at 405 nm;
protein concentrations were measured by the Bradford method with a bovine gamma-globulin standard.
SAMPLING
- Sampling frequency: every 5 minutes
Reference substance
- Reference substance:
- not specified
Results and discussion
- Test performance:
- Approximately 30 organisms were isolated from contaminated metalworking fluids. Most were gram-negative rod-shaped bacteria. Four organisms were selected for the study: AV1, CL1, FM2 and SP1.
- Transformation products:
- yes
Identity of transformation productsopen allclose all
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- alcohol
- Identifier:
- common name
- Identity:
- alcohol
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- aldehyde
- Identifier:
- common name
- Identity:
- aldehyde
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- phosphate
- Inventory number:
- InventoryMultipleMappingImpl [inventoryEntryValue=EC 604-302-9]
- Identifier:
- common name
- Identity:
- phosphate
- Details on results:
- Bacteria isolated from contaminated metalworking fluids:
AV1 (Flavobacterium) reduced nitrate, had urease activity, assimilated glucose, arabinose, mannose, N-acetyl-glucosamine, maltose and malate.
CL1 (Bacillus) reduced nitrate, had beta-glucosidase, beta-galactosidase activities and assimilated glucose, arabinose, mannose, mannitol, maltose, gluconate, malate and citrate.
FM2 (Aeromonas) produced indole and acid, had beta-glucosidase activity and assimilated N-acetylglucosamine, maltose and citrate.
SP1 (Pseudomonas) assimilated glucose, arabinose, mannose, N-acetylglucosamine, maltose and malate.
Formation of products from sodium diethyl dithiophosphate (NaDDP):
Extracts of all the organisms studied produced alcohol, aldehyde and phosphate in excess. No products were found in control experiments where boiled extracts were used or where the extract was omitted. In all cases, ethanol was present in lower detectable quantities than aldehyde or phosphate. Of the four organisms studied, Flavobacterium (AV1) appeared to catabolize the test material most rapidly. However, only 10% of the total phosphate of the NaDDP molecle was realized during the 2 hour study period. No hydrogen sulphide production by any of the four organisms was detected under the assay conditions used. Neither was alkaline phosphodiesterase activity detected. - Results with reference substance:
- Not applicable
Any other information on results incl. tables
No other information.
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The breakdown of sodium O,O-diethyl dithiophosphate by four bacterial strains (Aeromonas, Pseudomonas, Flavobacterium and Bacillus) isolated from contaminated metalworking fluids was shown to involve the successive formation of ethanol, aldehyde and orthophosphate.
- Executive summary:
Four bacterial strains were isolated from contaminated metalworking fluids and subsequently identified as Aeromonas, Pseudomonas, Flavobacterium and Bacillus.
These bacterial strains were cultured in the presence of the test substance sodium O,O-diethyl dithiophosphate and samples were taken every 5 minutes over a 2 hour period and examined for degradation products of the test substance.
Ethanol, aldehyde and orthophosphate were identified as breakdown products of the test substance.
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