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EC number: 206-055-9 | CAS number: 298-06-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study fulfiled the criteria of validation as determined by the non-standard guideline.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DS/EN ISO 14669 " water quality - determination of acute lethal toxicity to marine copepods (Copopeda, Crustacea)", 1999
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phosphorodithioic acid, O,O-diethylester, potassium salt
- IUPAC Name:
- Phosphorodithioic acid, O,O-diethylester, potassium salt
- Reference substance name:
- 3454-66-8
- Cas Number:
- 3454-66-8
- IUPAC Name:
- 3454-66-8
- Details on test material:
- - Name of test material (as cited in study report): EP-1, potassium salt (Potassium O,O-diethyl phosphorodithioate)
- Molecular formula (if other than submission substance): C4H10KO2PS2
- Molecular weight (if other than submission substance):224.32 g/mol
- Physical state: white powder
- Analytical purity: 99.7% (w/w)
- Lot/batch No.: 343-BSe-66E
- Storage condition of test material: Test substance was stored at -20°C.
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): 80 g/L
- log Pow: -0.46
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by dissolving the test substance directly in test medium (filtered clean sea water), from which the test solutions were prepared
- Controls: Filtered clean sea water without test substance
Test organisms
- Test organisms (species):
- other aquatic crustacea: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Name:Acartia tonsa (Dana)
- Source: Originally collected in the North Sea by Danish Institute for Fisheries Research and cultured at DHI Water & Environment (since 1987); Animals were taken from this culture.
- Age at study initiation: Animals were 2-3 weeks of age
Study design
- Test type:
- not specified
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Not applicable
- Test temperature:
- 19.5°C +/- 0.5°C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- Oxygen saturation was 96% even at the highest test substance concentration.
- Salinity:
- 32%
- Nominal and measured concentrations:
- nominal: 0, 100, 200, 500, 1000 and 2000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL glass beakers with 25 mL test solution
- No. of organisms per vessel: 7 adults
- No. of vessels per concentration (replicates): 3 per concentration
- No. of vessels per control (replicates): 5 beakers with 7 animals each
OTHER TEST CONDITIONS
- Photoperiod: light/dark period of 16:8
TEST CONCENTRATIONS
- Test concentrations: 0, 100, 200, 500, 1000 and 2000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-DCP
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC10
- Effect conc.:
- 529 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 271 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality of control: < 10%
- Results with reference substance (positive control):
- - LC50: 1.39 mg/L after 48 hours
- LC10: 0.36 mg/L after 48 hours
- LC90: 2.42 mg/L after 48 hours - Reported statistics and error estimates:
- Not applicable
Any other information on results incl. tables
The validation criteria of the test according to the DS/EN ISO 14669, 1999 are considered fulfilled:
- At the end of the test, the O2 -saturation was 96% even at the highest test concentration.
- The mortality of the control group was < 10%.
- The toxicity of the reference chemical (3,5 -DCP) revealed an LC50 of 1.39 mg/L (range: 1.16 - 1.72 mg/L) and was within the specified range of 0.5 - 1.5 mg/L.
Mean LC10, LC50 and LC90 values for treatment with EP-1, potassium salt:
Endpoint |
24 hours |
48 hours |
LC10 [mg/L] |
1106 |
529 |
LC50 [mg/L] |
> 2000 |
1271 |
LC90 [mg/L] |
> 2000 |
> 2000 |
Mean LC10, LC50 and LC90 values for treatment with 3,5 -DCP:
Endpoint |
24 hours |
48 hours |
LC10 [mg/L] |
1.05 |
0.36 |
LC50 [mg/L] |
2.11 |
1.39 |
LC90 [mg/L] |
3.13 |
2.42 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute toxicity test in Acartia tonsa, after 48 hours of exposure towards EP-1, potassium salt, an LC50 value of 1271 mg/L was obtained.
- Executive summary:
The test substance "EP-1, potassium salt" was tested for the acute toxic effects on the crustacean Acartia tonsa in accordance with the DS/EN ISO 14669 guideline of 1999.
The test was carried out in 50 mL test beakers with 25 mL of test solution in each beaker. The number of dead animals was counted after 24 and 48 hours; total exposure time was 48 hours.
A stock solution of EP-1 potassium salt was prepared by dissolving 400 mg of the test substance in 200 mL seawater, thereby generating a conentration of 2000 mg/L of EP-1 potassium salt. From this, the follwing test concentrations were generated (dilution in seawater): 100, 200, 500, 1000 and 2000 mg/L. Seawater without test substance served as negative control. As reference substance, 3,5 -DCP was used, to test the sensitivity of the test organism.
Chemical analysis revealed that the actual concentration in the stock solution was 1960 mg/L which is equivalent to the nominal concentration. The toxicity of the reference substance was within the expected range.
The results of the study expressed in nominal concentrations can be summarized as follows:
LC10 = 529 mg/L after 48 hours of exposure,
LC50 = 1271 mg/L, after 48 hours of exposure,
LC90 > 2000 mg/L, after 48 hours of exposure.
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