Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: sediment simulation testing
- Remarks:
- The test addresses both the surface water and sediment compartments
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 September 2003 to 26 May 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 308 (Aerobic and Anaerobic Transformation in Aquatic Sediment Systems)
- Deviations:
- yes
- Remarks:
- (the study integrity was not affected by the deviations)
- GLP compliance:
- yes
- Radiolabelling:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water / sediment
- Details on source and properties of surface water:
- System Goorven Englese Dijk
Total N 2.8 mg/L 1.4 mg/L
Total P 0.1 mg/L 0.1 mg/L
Hardness: 29 mg/L CaCO3 126 mg/L CacO3
DOC: 14.6 mg/L (start); 9.7 mg/L (end) 19.5 mg/L (start); 4.9 mg/L (end)
pH: 5.44 7.46
Temperature: 18 ºC 16.7 ºC
Redox potential: 157 mV 39 mV
Oxygen: 7.9 mg/L 7.19 mg/L - Details on source and properties of sediment:
- System Goorven Englese Dijk
Sand (63µm-2mm) 99.37 % 17.97 %
Silt (2µm-63µm) 0.56 % 49.97 %
Clay (<2µm) 0.07 % 32.06 %
Texture Sand Silty clay loam
Organic carbon 1.2% (start); 0.5 % (end); 0.8 % (mean) 5.4% (start); 7.2 % (end); 6.3 % (mean)
pH (water) 6.2 (start); 5.4 (end) 7.5 (start); 7.3 (end)
pH (KCl) 5.2 (start); 4.6 (end) 7.3 (start, end)
pH (CaCl2) 5.7 (start); 4.6 (end) 7.5 (start); 7.1 (end)
CEC 1.1 mEq/100g 45.2 mEq/100g
Total N 448 mg/kg 922.5 mg/kg
Total P 36.4 mg/kg 2165.4 mg/kg - Duration of test (contact time):
- 100 d
- Initial conc.:
- 2.7 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- EQUILIBRATION:
For the Goorven system, approximately 250 g wet sediment and 450 mL water were each placed in 24 1 L Brown metabolism flasks. For the Engelse Dijk system, approximately 125 g wet sediment and 470 mL water were each placed in 24 1 L Brown metabolism flasks. The wet sediment weights and water volumes were each chosen so that a sediment later of approximately 2 cm and a water layer of approximately 6 cm was obtained. The metabolism flasks were placed in a climatised room at ~20ºC and the next day connected to an air stream which was allowed to bubble gently through the upper layer of the water during the equilibration period. The equilibration period last 12 days for the Goorven system and 20 days for the Engelse Dijk system.
INCUBATION
450 µL of a spiked solution (equivalent to 2.7 mg test material/L) was added to 18 metabolism flasks for each system. Immediately after spiking, the metabolism flasks were placed in the climatised room in the dark and connected to a series of traps: (1) a polyurethane foam flask inserted in the neck of the flask, (2) a liquid trap containing 2-methoxyethanol and (3) two liquid traps containing 2N NaOH. During incubation, aeration took place twice daily for 30 minutes. The ingoing air was allowed to bubble gently through the upper part of the water layer so as to not disturb the sediment layer (see Figure 1).
SAMPLING
Two flasks of each system were harvested at T=0 (30 minutes after spiking), 3, 7, 15, 30, 59 and 100 days after spiking. At sampling, the polyurethane foam plugs and liquid traps were also analysed and replaced in the flasks not being sampled to avoid saturation.
WATER LAYER
The water layer was carefully decanted and weighed. Radioactivity in the water and extracts generated by extraction with dichloromethane were analysed by LSC.
SEDIMENT LAYER
The sediment later was added to a centrifuge bottle and the metabolism flask rinsed with 100 mL methanol which was added to the sediment for 30 minutes extraction on a shaker. The supernatant was centrifuged for 5 minutes at 1761 g. This procedure was repeated twice more. Radioactivity was determined in the methanol extracts by LSC. The post-extraction sediment was transferred to a Soxhlet apparatus and extracted with 250 mL methanol for 3 hours. Total radioactivity in the Soxhlet extract was determined by LSC. The remaining sediment was air-dried for combustion analysis.
POLURETHANE PLUGS
The plugs were twice extracted with acetonitrile and radioactivity in the extracts determined by LSC.
LIQUID TRAPS
Total radioactivity was determined by LSC. - Test performance:
- Redox potential and oxygen concentration measurements indicated aerobic conditions in the water layer and anaerobic conditions in the sediment throughout the test.
The mass balances for the Goorven system ranged from 97.20 to 100.18 % of applied radioactivity and from 97.54 to 101.45 % for the Engelse Dijk system and fulfil the guideline criteria of 90-110 %. - Key result
- Compartment:
- sediment
- DT50:
- >= 10.1 - <= 10.9 d
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Key result
- Compartment:
- sediment
- DT50:
- >= 3.5 - <= 6.3 d
- Type:
- other: hockystick model
- Temp.:
- 20 °C
- Transformation products:
- yes
- No.:
- #1
- Details on transformation products:
- Metabolites in the sediment were 2,6-dichlorobenzamide (maximum 1.73 (GV) - 2.04 % (ED) of applied) and four unidentified minor metabolites (in ED system only), none of which exceeded 10 % of applied radioactivity or 5 % of applied radioactivity at two consecutive time points (individual maximum 3.86 % (ED)). See Tables 3 and 4.
- Evaporation of parent compound:
- yes
- Volatile metabolites:
- not specified
- Residues:
- not specified
- Details on results:
- The fate of the test material in water/sediment systems can be described as follows: Upon addition to the water, a rapid partitioning of the test material between water and sediment took place in both water/sediment systems. The test material in the water phase was removed through volatilisation (major process, 27.08 (ED) - 36.40 % (GV)) and degradation (7.80 (GV) - 20.78 % (ED)), which caused a continuous repartitioning of the test material from the sediment to the water. Some degradation of the test material in the sediment was observed (1.73 (GV) - 9.29 % (ED)). Bound residues accounted for 3.03 (GV) - 7.83 % (ED) and complete mineralisation accounted for 0.63 (GV) - 4.51 % (ED) of applied radioactivity after 100 days.
The rate of test material dissipation in the water, sediment and combined water and sediment compartments could be described by first order kinetics and/or multi-compartment first order kinetics and/or the hockey stick model (see Table 1 and 2). - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the study, the test material in the water phase is removed through volatilisation (major process) and degradation. The dissipation half-life of the test material in the aqueous phase ranges from 10.1 - 10.9 days, based on first order kinetics and 3.5 - 6.3 days, based on the hockystick model.
Metabolites in the water layer were 2,6-dichlorobenzamide, 2-chlorobenzoic acid (only in one of two test systems) and eight unidentified minor metabolites, none of which exceeded 10 % of applied radioactivity or 5 % of applied radioactivity at two consecutive time points. - Executive summary:
In a GLP compliant water/sediment study conducted in line with standardised guideline OECD 308, the degradation of the test material in two water/sediment systems was determined. The test material was incubated in the laboratory in two non-contaminated water/sediment systems at 20 ± 2 ºC for 100 days. The initial test material concentration in the water layer was approximately 2.7 mg/L. Replicate samples of each water/sediment system were taken at 0, 3, 7, 15, 30, 59 and 100 days. The test material in the water phase is removed through volatilisation (major process) and degradation.
The dissipation half-life of the test material in the aqueous phase ranges from 10.1 - 10.9 days, based on first order kinetics and 3.5 - 6.3 days, based on the hockystick model.
Metabolites in the water layer were 2,6-dichlorobenzamide, 2-chlorobenzoic acid (only in one of two test systems) and eight unidentified minor metabolites, none of which exceeded 10 % of applied radioactivity or 5 % of applied radioactivity at two consecutive time points.
Reference
Table 1: Degradation rates of the test substance in the 'Goorven' water/sediment test system
Compartment | Process | DT50 (days) | DT75 (days) | DT90 (days) | r2 * | |
Water | Volatilisation + transfer to sediment + degradation | 10.9 | 21.7 | 36.1 | 0.814 | |
3.521, 39.4, 3.52 | 7.041, 78.8, 35.8 | 11.71, 131, 87.7 | 0.988 | |||
Degradation | 233 | 467 | 775 | 0.989 | ||
Water + Sediment | Volatilisation + degradation | 100 | 201 | 333 | 0.997 | |
Degradation | 3582, 307 | 7172, 614 | 11912, 1020 | 0.999, 0.973 | ||
Sediment | Degradation + repartitioning to water | 152 | 304 | 505 | 0.948 | |
Degradation | 737 | 1475 | 2450 | 0.989 |
1hockey stick model: before hinge point, after hinge point, overall
2based on 1st order multi-compartment model, and normalised data/1st order
*coefficient of determination
Table 2: Degradation rates of the test material in the 'Engelse Dijk' water/sediment test system
Compartment | Process | DT50(days) | DT75(days) | DT90(days) | r2 * | |
Water | Volatilisation + transfer to sediment + degradation | 10.1 | 20.3 | 33.7 | 0.934 | |
6.261, 28.6, 6.26 | 12.51, 57.3, 26.8 | 20.71, 95.1, 64.6 | 0.991 | |||
Degradation | 56.6 | 113 | 188 | 0.986 | ||
Water + Sediment | Volatilisation + degradation | 47 | 94 | 156 | 0.975 | |
Degradation | 72.62, 65.6 | 1452, 131 | 2412, 218 | 0.990, 0.962 | ||
Sediment | Degradation + repartitioning to water | 68.6 | 137 | 228 | 0.925 | |
Degradation | 119 | 237 | 394 | 0.986 |
1hockey stick model: before hinge point, after hinge point, overall
2based on 1st order multi-compartment model, and normalised data/1st order
*coefficient of determination
Table 3: Summary of the quantitation of [14C]-dichlobenil and metabolites in water, sediment and polyurethane foam (PUF) for the Goovern system (% of applied)
Water | Sediment extractables | Volatiles (PUF) | |||||||
Time (days) | Replicate | Test material | BAM | others | Test material | BAM | others | Test material | Others |
0 | A | 97.30 | 0.00 | 1.45 | 1.32 | 0.00 | 0.00 | 0.00 | 0.00 |
B | 97.65 | 0.00 | 1.28 | 1.51 | 0.00 | 0.00 | 0.00 | 0.00 | |
mean | 94.78 | 0.00 | 1.36 | 1.41 | 0.00 | 0.00 | 0.00 | 0.00 | |
3 | A | 54.12 | 0.00 | 0.68 | 41.29 | 0.00 | 0.00 | 2.97 | 0.00 |
B | 53.88 | 0.00 | 0.67 | 40.72 | 0.00 | 0.00 | 4.01 | 0.00 | |
mean | 54.00 | 0.00 | 0.67 | 41.00 | 0.00 | 0.00 | 3.49 | 0.00 | |
7 | A | 44.96 | 0.00 | 0.54 | 48.61 | 0.00 | 0.00 | 5.41 | 0.00 |
B | 45.88 | 0.00 | 1.19 | 47.11 | 0.00 | 0.00 | 5.28 | 0.00 | |
mean | 45.42 | 0.00 | 0.87 | 47.86 | 0.00 | 0.00 | 5.34 | 0.00 | |
15 | A | 30.35 | 0.56 | 0.85 | 55.95 | 0.00 | 0.00 | 9.90 | 0.00 |
B | 30.43 | 0.30 | 0.73 | 55.19 | 0.00 | 0.00 | 9.06 | 0.00 | |
mean | 30.39 | 0.43 | 0.79 | 55.57 | 0.00 | 0.00 | 9.48 | 0.00 | |
30 | A | 23.53 | 1.17 | 1.03 | 58.50 | 0.00 | 0.00 | 12.87 | 0.00 |
B | 25.31 | 1.17 | 1.12 | 51.99 | 0.00 | 0.00 | 18.03 | 0.00 | |
mean | 24.42 | 1.17 | 1.08 | 55.24 | 0.00 | 0.00 | 15.45 | 0.00 | |
59 | A | 17.80 | 2.96 | 2.53 | 50.85 | 0.00 | 0.00 | 22.46 | 0.00 |
B | 15.79 | 2.68 | 2.43 | 46.55 | 0.00 | 0.00 | 28.15 | 0.00 | |
mean | 16.80 | 2.82 | 2.48 | 48.70 | 0.00 | 0.00 | 25.31 | 0.00 | |
100 | A | 11.44 | 4.11 | 2.85 | 38.93 | 1.30 | 0.00 | 36.37 | 0.00 |
B | 10.84 | 4.82 | 3.83 | 36.20 | 2.16 | 0.00 | 36.44 | 0.00 | |
mean | 11.14 | 4.46 | 3.34 | 37.56 | 1.73 | 0.00 | 36.40 | 0.00 |
BAM = 2,6-dichlorobenzamide
others = includes residual (post-extraction) radioactivity in the water layer and up to four individual metabolites all ≤0.61% of applied
Table 3: Summary of the quantitation of [14C]-dichlobenil and metabolites in water, sediment and polyurethane foam (PUF) for the Englese Dijk system (% of applied)
Water | Sediment extractables | Volatiles (PUF) | ||||||||
Time (days | Replicate | Test material | BAM | others | 2-CBA | Test material | BAM | others | Test material | others |
0 | A | 97.69 | 0.00 | 2.13 | 0.00 | 2.50 | 0.00 | 0.00 | 0.00 | 0.00 |
B | 98.99 | 0.00 | 2.05 | 0.00 | 1.63 | 0.00 | 0.00 | 0.00 | 0.00 | |
mean | 98.34 | 0.00 | 2.09 | 0.00 | 2.07 | 0.00 | 0.00 | 0.00 | 0.00 | |
3 | A | 61.04 | 0.00 | 0.92 | 0.00 | 27.64 | 0.00 | 2.57 | 5.74 | 0.18 |
B | 66.77 | 0.00 | 1.05 | 0.00 | 26.04 | 0.00 | 1.46 | 3.73 | 0.00 | |
mean | 63.90 | 0.00 | 0.98 | 0.00 | 26.84 | 0.00 | 2.02 | 4.74 | 0.09 | |
7 | A | 47.22 | 0.36 | 1.82 | 0.00 | 42.20 | 0.00 | 1.01 | 6.95 | 0.00 |
B | 48.33 | 0.26 | 1.92 | 0.00 | 42.53 | 0.00 | 1.44 | 6.83 | 0.00 | |
mean | 47.77 | 0.31 | 1.87 | 0.00 | 42.36 | 0.00 | 1.23 | 6.89 | 0.00 | |
15 | A | 31.77 | 0.79 | 4.39 | 0.00 | 46.97 | 0.00 | 36.7 | 10.21 | 0.23 |
B | 34.40 | 0.62 | 3.62 | 0.00 | 47.14 | 0.00 | 1.87 | 10.79 | 1.03 | |
mean | 33.08 | 0.70 | 4.00 | 0.00 | 47.06 | 0.00 | 2.77 | 10.50 | 0.63 | |
30 | A | 21.33 | 2.39 | 8.07 | 2.10 | 41.60 | 1.44 | 4.54 | 15.39 | 0.00 |
B | 20.13 | 4.38 | 7.12 | 4.60 | 38.42 | 2.09 | 2.29 | 17.80 | 0.00 | |
mean | 20.73 | 3.38 | 7.59 | 3.35 | 40.01 | 1.76 | 3.41 | 16.59 | 0.00 | |
59 | A | 11.54 | 2.53 | 14.77 | 4.79 | 29.02 | 0.00 | 7.36 | 20.81 | 0.00 |
B | 8.08 | 5.71 | 6.69 | 13.47 | 21.29 | 3.82 | 6.28 | 21.00 | 0.00 | |
mean | 9.81 | 4.12 | 10.73 | 9.13 | 25.15 | 1.91 | 6.82 | 20.91 | 0.00 | |
100 | A | 7.84 | 4.20 | 7.44 | 43.8 | 25.44 | 2.18 | 7.78 | 25.60 | 2.43 |
B | 6.64 | 6.45 | 7.66 | 11.45 | 20.03 | 1.90 | 6.72 | 26.13 | 0.00 | |
mean | 7.24 | 5.32 | 7.55 | 7.91 | 22.74 | 2.04 | 7.25 | 25.87 | 1.21 |
BAM = 2,6-dichlorobenzamide
2 -CBA = 2-chlorobenzoic acid
others = includes residual (post-extraction) radioactivity in the water layer and up to four individual metabolites all ≤0.61% of applied
Description of key information
The half-life of the test material was determined to be 10.1-10.9 days (first order kinetics) or 3.5-6.3 days (hockystick model) according to a study performed in line with OECD Guideline 308.
Key value for chemical safety assessment
Additional information
See 'Environmental Fate and Pathways' endpoint summary.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.