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EC number: 801-277-8 | CAS number: 507448-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
- EC Number:
- 801-277-8
- Cas Number:
- 507448-65-9
- Molecular formula:
- C7H10F3NO2
- IUPAC Name:
- ethyl (2Z)-4,4,4-trifluoro-3-(methylamino)but-2-enoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
Water samples of each concentration (2 x 100 m volume each) were taken from the freshly prepared test solutions at the start of exposure.
At test termination (48 hours) 2 samples of 50 ml and 2 samples of 100 ml were taken from all 4 replicates of each concentration.
All samples were kept at -18 °C to -22 ° C until analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The specimens were thawed in a microwave oven at ambient temperature. After intensively shaking, the measured specimens were diluted with methanol in the ratio 1:1. The final volume was measured to calculate the dilution factor. These solutions were filtered through a 0.45 µm filter before injection.
24.6 – 66.8 mg CA 2245 A were weighed, dissolved and made up to 25.0 ml with methanol. From these stock solutions, reference solutions in the range of the test concentrations (related to and extended to about 10 % below and above the expected concentration) were diluted with methanol/test water = 1/1 (v/v). So references with concentrations between 0.20 – 13.36 mg/L CA 2455 A were used.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Species: Daphnia magna STRAUS 1820
- Strain: Clone 5
- Source: In-house
- Age at study initiation: < 24 hours
Study design
- Test type:
- static
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Water hardness corrsponded to 260 and 240 mg CaCO3/L (2 bottles).
- Test temperature:
- 21 ± 1 °C
- pH:
- 8.5 and 8.0 – 8.1 at the start and at the end of exposure, respectively.
- Dissolved oxygen:
- 100 -104 % and 92 – 95 % at the start and at the end of exposure, respectively.
- Salinity:
- Conductivity was measured to be 642 and 663 µS (2 bottles)
- Nominal and measured concentrations:
- Nominal Concentrations: 0.50, 1.0, 2.0, 4.0, 8.0 and 16.0mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 ml glass beakerss containing 100 ml of test solution or blank test medium
- No. of animals per concentration: 20 animals for each concentration and the control
- No. of organisms per vessel: 5 animals exposed per test vessel and the control
- No. of vessels per concentration: 4 test vessels for each concentration
- No. of vessels per control: 4 test vessels for the control
TEST MEDIUM / WATER PARAMETERS
- Test medium: M4- medium according to ELENDT.
OTHER TEST CONDITIONS
- Photoperiod: Light-dark cycle of 16 hours light and 8 hours datk with a 30 minutes transition period.
- Light intensity: Fluorescent light (approx. 1100 lux).
EFFECT PARAMETERS MEASURED
- Oxygen content: At start and test end (48 hours).
- pH: At start and test end (48 hours).
- Temperature: At start and test end (48 hours).
- Hardness: From the freshly prepared M4-medium.
- Conductivity: From the freshly prepared M4-medium.
TEST CONCENTRATIONS
- Test concentrations: Nominal 0.50, 1.0, 2.0, 4.0, 8.0 and 16.0mg/L
BIOLOGICAL OBSERVATIONS
- Observation intervals: Immobilization and other behavioural changes of the daphnids were recorded after 24 and 48 hours of
exposure.
Those animals not able to swim within 15 seconds after gentle agitation of the test container were
considered to be immobile.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 6.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL
- Details on results:
- - Test concentrations: The measured test concentrations of CA 2455 A were 0.38, 0.74, 1.6, 3.1, 5.9 mg/L and LOD
(limit of detection, test item was not homogeneously distributed at the highest concentration)
at the start of exposure and(test item is not hydrolytical stable).
- Other observations: Despite the fact, that the test item is not miscible with water, the initial recovery directly after
application was between 70 and 80 % of nominal concentration (except the highest concentration).
The solubility of the test item and its physical characteristics did not allow to achieve the nominal test
concentrations over the whole test period.
- Other biological observations: No immobilization of daphnids was observed in the controls and at test concentrations of 0.50, 1.0
and 2.0 mg/L. After exposure for 48 hours 24, 60 and 95 % of daphnids were immobilized at test
concentrations of 4.0, 8.0 and 16.0 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
The EC 50 (48h) of CA 2455 A was determined to be 6.5 mg/L based on nominal concentrations.
The highest concentration with no observed effects after 48 hours was 2.0 mg/L based on nominal concentrations of CA 2455 A.- Executive summary:
Test concentrations of nominal 0.50, 1.0, 2.0, 4.0, 8.0 and 16.0mg/L of CA 2455 A were selected based on results of a range-finding test and a preliminary test. Exposure started when the daphnids were placed in the test vessel containing the test solutions.
Twenty daphnids were exposed to each of the desired test concentrations under static conditions in M4-medium. For the test concentrations and the control, four replicates were performed. Each replicate consisted of five specimens in 100ml test solution. The daphnids were observed for immobilization and behavioural changes after 24 h and 48 h of exposure.
The water temperature was maintained constant throughout the duration of the test at 21±1 °C in all test concentrations. The oxygen saturation was measured to be 100 -104 % and 92 – 95 % at the start and at the end of exposure, respectively. The pH was 8.5 and 8.0 – 8.1 at the start and at the end of exposure, respectively.
The measured test concentrations of CA 2455 A were 0.38, 0.74, 1.6, 3.1, 5.9 mg/L and LOD (limit of detection, test item was not homogeneously distributed at the highest concentration) at the start of exposure and <LOQ, 0.05, 0.16, 0.27 and 1.7 mg test item/L at the end of exposure (test item is not hydrolytical stable).
Despite the fact, that the test item is not miscible with water, the initial recovery directly after application was between 70 and 80 % of nominal concentration (except the highest concentration).
No immobilization of daphnids was observed in the controls and at test concentrations of 0.50, 1.0 and 2.0 mg/L. After exposure for 48 hours 24, 60 and 95 % of daphnids were immobilized at test concentrations of 4.0, 8.0 and 16.0 mg/L.
Following values are based on nominal concentrations.
Exposure period
EC 50
95 % confidence limit
Slope
No effect level (observed)
Hours
mg/L
mg/L
mg/L
24
48
8.0
6.5
6.5 – 10.2
5.3 – 8.1
1.71
1.78
2.0
2.0
Conclusion:
The EC 50 (48h) of CA 2455 A was determined to be 6.5 mg/L based on nominal concentrations.
The highest concentration with no observed effects after 48 hours was 2.0 mg/L based on nominal concentrations of CA 2455 A.
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