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Diss Factsheets
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EC number: 200-291-6 | CAS number: 56-84-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- 1. Characterization of the test substance by the manufacturer was not performed under Good Laboratory Practice Standards. 2. The dosing preparation used in the study was not analyzed for stability, homogeneity, or accuracy of concentration. The procedures
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- Aspartic acid
- EC Number:
- 200-291-6
- EC Name:
- Aspartic acid
- Cas Number:
- 56-84-8
- Molecular formula:
- C4H7NO4
- IUPAC Name:
- aspartic acid
- Details on test material:
- Substance Tested: ¿ L-Aspartic Acid
¿ 56-84-8 (CAS Number)
Haskell Number: 27761
Purity: ¿ 99.5%
Physical Characteristics: White solid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- 1. Housing
All animals were housed individually in suspended, stainless steel, wire-mesh cages.
2. Environmental Conditions
Animal rooms were maintained at a temperature of 18-26°C and a relative humidity of 30-70%. Animal rooms were artificially illuminated (fluorescent light) on an approximate 12-hour light/dark cycle.
3. Feed and Water
Tap water was available ad libitum. PMI® Nutrition International, LLC Certified Rodent LabDiet® 5002 was available ad libitum except during the fasting period.
4. Identification
Each rat was assigned an animal number which was recorded on a card affixed to the cage. The rats were tail-marked, using a water-insoluble marker, with the animal number.
5. Quarantine
Rats were observed daily for general health and weighed at least once during the 6-day quarantine period.
6. Animal Health and Environmental Monitoring Program
As specified in the Haskell Laboratory animal health and environmental monitoring program, the following procedures are performed periodically to ensure that contaminant levels are below those that would be expected to impact the scientific integrity of the study:
¿ Water samples are analyzed for total bacterial counts, and the presence of coliforms, lead, and other contaminants.
¿ Samples from freshly washed cages and cage racks are analyzed to ensure adequate sanitation by the cagewashers.
Certified animal feed is used, guaranteed by the manufacturer to meet specified nutritional requirements and not to exceed stated maximum concentrations of key contaminants, including specified heavy metals, aflatoxin, chlorinated hydrocarbons, and organophosphates. The presence of these contaminants below the maximum concentration stated by the manufacturer would not be expected to impact the integrity of the study. The animal health and environmental monitoring program is administered by the attending laboratory animal veterinarian. Evaluation of these data did not indicate any conditions that affected the validity of the study.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Doses:
- The rats were fasted approximately 18 hours prior to dosing. L-Aspartate was suspended in 0.5% aqueous methylcellulose on an as-is, weight per volume basis (approximately 15,000 mg test substance in 30 mL vehicle). The test substance was administered in a single oral dose by
gavage at a dose amount of 5000 mg/kg and a volume of 10 mL/kg body weight. The amount of dosing suspension administered to each animal was based on the animal¿s fasted body weight
determined on the day of dosing. The dosing suspension was stirred throughout the dosing
procedure to maintain homogeneity. The rats were approximately 10 weeks old on the day of
dosing. - No. of animals per sex per dose:
- 5
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Mortality:
- All rats survived for the duration of the study.
- Clinical signs:
- other: High carriage was observed in one male rat on the day of dosing. No clinical signs were observed in any of the other rats.
- Gross pathology:
- No gross lesions were found in the study.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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