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Diss Factsheets
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EC number: 229-239-0 | CAS number: 6443-69-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): 3,4,5-Trimethoxytoluol
- Physical state: yellowish, solid
- Analytical purity: 96.6%
- Lot/batch No.: 30364/143
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 12.5, 25, 50, 100 mg/L
- Sampling method: at the begin (after 0 h) and at the end of the exposition (after 48 h) samples from vessels without daphnids were analysed
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The concentration of the stock solution was 100 mg/L (net weight : 50.3 mg/500 ml). The stock solution was treated in an ultrasonic bath for approx. 15 min and then stirred for approx. 30 min
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology
- Age at study initiation: 2 - 24 h (starting with the 3rd breed of parent animals)
- Feeding during test: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.42 mmol/L
- Test temperature:
- 19.5 - 20.1 °C
- pH:
- 7.8 - 8.1
- Dissolved oxygen:
- 8.5 - 9.3 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 12.5, 25, 50, 100 mg/L
Measured test concentrations (0 h): n.d., 13.4, 24.9, 49.3, 135.9 mg/L
Measured test concentrations (48 h): n.d., 12.5, 24.9, 49.0, 95.8 mg/L
n.d. = non-detectable - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: a synthetic fresh water was used for culture and test purposes (M4 medium)
- Alkalinity: 0.87 mmol/L
- Conductivity: 608 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement:
- Temperature: Continuously during the whole test period in a separate vessel close the test vessels
- pH, oxygen content: after 0 h in an additional replicate without daphnids and after 48 h in replicate 1 of each tested concentration
OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm 16:8 h
- Light intensity: artificial light, type warm white; about 1-8 µE/(m²/s) at a wave length of 400 - 750 nm
EFFECT PARAMETERS MEASURED: mobility after 0, 24, 48 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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