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EC number: 204-337-6 | CAS number: 119-61-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: A standard NTP (National Toxicology Program) protocol. Equivalent to OECD 471.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 982
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 986
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (only four strains tested)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzophenone
- EC Number:
- 204-337-6
- EC Name:
- Benzophenone
- Cas Number:
- 119-61-9
- Molecular formula:
- C13H10O
- IUPAC Name:
- benzophenone
- Details on test material:
- - Name of test material (as cited in study report): Benzophenone
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mixture (metabolic activation enzymes and cofactors from Aroclor 1254-induced male Sprague-Dawley rat or Syrian hamster liver)
- Test concentrations with justification for top dose:
- 0 (control), 1, 3, 10, 33, 100, 166, 333 and 1000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- With metabolic activation
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- Strains TA1537, TA98, TA100, TA1535
- Positive controls:
- yes
- Remarks:
- Without metabolic activation
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Strain TA1537
- Positive controls:
- yes
- Remarks:
- Without metabolic activation
- Positive control substance:
- sodium azide
- Remarks:
- Strains TA100, TA1535
- Positive controls:
- yes
- Remarks:
- Without metabolic activation
- Positive control substance:
- other: 4-nitro-o-penhylenediamine
- Remarks:
- Strain TA98
- Details on test system and experimental conditions:
- In the standard protocol (preincubation) for conducting the Ames assay, a test tube containing a suspension of one strain of Salmonella typhimurium plus S9 mix or plain buffer without S9, is incubated for 20 minutes at 37 ºC with the test chemical. Control cultures, with all the same ingredients except the test chemical, are also incubated. In addition, positive control cultures are prepared; these contain the particular bacterial tester strain under investigation, the various culture ingredients, and a known potent mutagen. After 20 minutes, agar is added to the cultures and the contents of the tubes are thoroughly mixed and poured onto the surface of Petri dishes containing standard bacterial culture medium (Vogel-Bonner Medium. The plates are incubated, and bacterial colonies that do not require an excess of supplemental histidine appear and grow. These colonies are comprised of bacteria that have undergone reverse mutation to restore function of the histidine -manufacturing gene. The number of colonies is usually counted after 2 days.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (stlight toxit at 1000 µg/plate in all strains with metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Benzophenone did not significatly increase the number of revertant colonies both in the absence or presence of a metabolic activation.
The substance was determined to be non-mutagenic under test conditions. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
TA100:
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
118 |
12.3 |
118 |
11.7 |
111 |
1.8 |
133 |
7.2 |
105 |
8.5 |
146 |
4.4 |
1 |
113 |
7.4 |
||||||||||
3 |
107 |
8.1 |
125 |
2.2 |
95 |
4.6 |
130 |
3.8 |
||||
10 |
110 |
10.3 |
132 |
7.7 |
102 |
7.3 |
136 |
4.1 |
90 |
6.6 |
131 |
4.3 |
33 |
100 |
6.4 |
123 |
2.8 |
84 |
3.9 |
128 |
10.7 |
96 |
4.2 |
112 |
11.7 |
100 |
110 |
4.7 |
114 |
9.8 |
78 |
6.3 |
154 |
7 |
99 |
7.5 |
124 |
1.9 |
166 |
52s |
7.5 |
||||||||||
333 |
80 |
4.1 |
117 |
8.7 |
86 |
7 |
90 |
6.6 |
||||
1000 |
50s |
6.1 |
35s |
10.9 |
||||||||
Positive Control |
383 |
14.9 |
297 |
16.9 |
1784 |
26.1 |
2174 |
37.4 |
922 |
112.2 |
1638 |
60.4 |
TA1535
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
36 |
1.9 |
32 |
2.3 |
11 |
2.1 |
16 |
1.8 |
13 |
3.5 |
6 |
1.2 |
1 |
33 |
2.5 |
||||||||||
3 |
37 |
0.7 |
30 |
3.2 |
9 |
1.7 |
9 |
1.7 |
||||
10 |
31 |
0.7 |
30 |
1.2 |
9 |
1.8 |
10 |
2.2 |
11 |
2.7 |
12 |
3 |
33 |
26 |
5.2 |
27 |
2 |
10 |
2.7 |
10 |
1.5 |
8 |
0.3 |
6 |
3.7 |
100 |
32 |
3.8 |
22 |
5.4 |
7 |
0.6 |
11 |
3 |
10 |
2.7 |
8 |
3.4 |
166 |
0s |
0 |
||||||||||
333 |
6 |
1.5 |
8 |
0.9 |
8 |
2.7 |
5 |
0.3 |
||||
1000 |
4 |
1 |
1s |
0.9 |
||||||||
Positive Control |
395 |
21.7 |
404 |
28.2 |
492 |
17.2 |
691 |
15.2 |
211 |
18.1 |
535 |
23 |
TA1537
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
4 |
0.9 |
7 |
0.3 |
9 |
0.9 |
7 |
0.6 |
7 |
0.3 |
6 |
1.2 |
1 |
6 |
2.1 |
||||||||||
3 |
5 |
0.7 |
5 |
1.8 |
8 |
2.3 |
7 |
2.4 |
||||
10 |
4 |
0.9 |
7 |
0.6 |
5 |
1.2 |
8 |
2.6 |
6 |
1.2 |
5 |
0.7 |
33 |
6 |
1.7 |
6 |
1.2 |
7 |
1.5 |
8 |
2.3 |
6 |
1.2 |
13 |
2 |
100 |
4 |
0.3 |
5 |
1.8 |
7 |
1.8 |
8 |
2.7 |
8 |
0.6 |
8 |
0.6 |
166 |
2s |
0.3 |
||||||||||
333 |
3 |
1.5 |
5 |
1.5 |
7 |
0.9 |
5 |
1.5 |
||||
1000 |
4 |
1.8 |
3s |
0.3 |
||||||||
Positive Control |
186 |
19.4 |
443 |
51.6 |
408 |
11.7 |
125 |
7.3 |
132 |
20.3 |
509 |
19.9 |
TA98
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
98 |
78 |
13 |
2.6 |
36 |
2.5 |
32 |
0 |
23 |
2.3 |
31 |
3.3 |
1 |
19 |
0.3 |
||||||||||
3 |
19 |
3.8 |
13 |
4.8 |
34 |
3.3 |
39 |
1.5 |
||||
10 |
19 |
1.9 |
10 |
2.4 |
30 |
2.8 |
34 |
4.5 |
33 |
1.3 |
30 |
0.7 |
33 |
20 |
2.3 |
17 |
0.9 |
31 |
2.7 |
36 |
4.2 |
21 |
2.4 |
27 |
7.5 |
100 |
14 |
1.9 |
12 |
2.2 |
30 |
3.2 |
33 |
4.4 |
28 |
5.5 |
27 |
1.2 |
166 |
0s |
0 |
||||||||||
333 |
23 |
1 |
15 |
1.2 |
25 |
4.5 |
14 |
3.2 |
||||
1000 |
15 |
2.1 |
6s |
0.3 |
||||||||
Positive Control |
475 |
5.4 |
431 |
38.4 |
1629 |
25.7 |
1901 |
39.4 |
867 |
11.9 |
1221 |
9.9 |
Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative (with and without metabolic activation)
Benzophenone did not significantly increase the number of revertant colonies both in the absence or presence of a metabolic activation. The substance was determined to be non-mutagenic under test conditions. - Executive summary:
A standard NTP (National Toxicology Program) protocol was performed to investigate the mutagenicity of benzophenone (test method equivalent to OECD 471). Different concentrations of test substance (from 100 to 10000 μg/plate) were tested in Salmonella typhimurium TA 1535, TA1537, TA100 and TA 98 by the preincubation assay (37°C, 20 min) with and without metabolic activation. Positive and negative controls confirmed the sensitivity of the test system. Benzophenone did not significantly increase the number of revertant colonies both in the absence or presence of a metabolic activation. The substance was determined to be non-mutagenic under test conditions.
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