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EC number: 209-025-3 | CAS number: 552-89-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Data from the literature in which all parameters described are closely comparable to a guideline method and well documented.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Rec assay
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- o-Nitrobenzaldehyde
- IUPAC Name:
- o-Nitrobenzaldehyde
- Reference substance name:
- 2-nitrobenzaldehyde
- EC Number:
- 209-025-3
- EC Name:
- 2-nitrobenzaldehyde
- Cas Number:
- 552-89-6
- Molecular formula:
- C7H5NO3
- IUPAC Name:
- 2-nitrobenzaldehyde
- Details on test material:
- - Name of test material (as cited in study report): o-Nitrobenzaldehyde
- Analytical purity: min. 99%
- Source: Tokyo Kasei Kogyo Co.
Constituent 1
Constituent 2
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- bacteria, other: Bacillus subtilis
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0.01, 0.05, 0.1, 0.5, 1, 5 mg (Dose/plate)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: sterilized dimethyl sulphoxide (DMSO)
- Source: Junsei Chemical Co
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- other: N-Ethyl-N'- nitro-N-nitrosoguanidine (ENNG), 2-aminoanthracene (2-AA)
- Details on test system and experimental conditions:
- Ames test:
METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk
DURATION
- Preincubation period: 0.1 ml of various concentrations of test compounds were added to a sterile test-tube containing 3 -6 x 10^7 bacerial cells, 0.5 ml of S9 mix or sodium phosphate buffer (pH 7.4). This mixture was preincubated in a shaker water-bath at 37°C for 15 min, then added to 2 ml molten top agar (45°C). The contents of each tube were mixed well and immediately poured onto the surface of a minimal-agar plate.
- Exposure duration: The plates were inverted and incubated at 37°C in the dark for 70 h.
- Control: A control plate contained 0.05 ml of DMSO.
- Contamination test: was carried out through each experiment.
- The background bacterial lawn: was routinely checked by microscopy
- Ampicilin resistance: TA 98 and TA 100 were checked routinely
NUMBER OF REPLICATIONS: all tests were performed in duplicate and repeated at least 3 times separately
Rec assay:
Rec assay according to procedures described by Kada et al. (1972) and Hirano et al. (1982).
In brief, liquid broth medium consisted of 10 g beef extract, 10 g polypeptone and 5 g NaCl per 100 ml. Minimal salt solution (MM) containing 1 g (NH4)2SO4, 10 g KH2PO4, 0.1 g MgSO4 x 7H2O and 0.5 g sodium citrate per 1000 ml (neutralized with KOH) was used for dilution and washing of bacteria. Overnight cultures of strains H17 and M45 in liquid broth medium were diluted 10 times with minimal salt solution. They were streaked from small pipettes onto the surface of a broth agar plate, care being taken not to let them touch one another. Paper discs, 13 mm in diameter, were prepared by punching filter papers. A disc has soaked in 0.05 ml of various concentrations of compounds and placed on the plate so as to cover the end of the bacterial streaks. After incubation for 24 h at 37°C , grown bacteria become visible except in the inhibition zone depending on the strain and on the compound used. When a compound showed higher inhibition to M45 (Rec-) than to H17 (Rec+) the assay was repeated. A difference of more than 1 mm was a positive response.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA 98, TA 1538, TA 1537, TA 100, TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Concentrations 1 and 5 mg/plate indicate toxic effects with and without metabolic activation in all test strains
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: Bacillus subtilis
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 2: Results for o-Nitrobenzaldehyde, data represent the results of 3 separate experiments and give the mean values of 3 plates. The numbers of revertant colonies indicate mean +- S.D., all these numbers of revertant colonies were counted after 68 - 72 h incubation.
Dose (/plate) | Ames test | Rec assay | |||||||||
TA98 | TA1538 | TA1537 | TA100 | TA1535 | (mm)** | ||||||
-S9 | +S9 |
-S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | ||
0.01 mg | 24 +-3 | 22 +-3 | 20 +-3 | 21 +-3 | 8 +-2 | 10 +-2 | 163 +-18 | 174 +-20 | 31 +-5 | 35 +-4 | |
0.05 | 28 +-4 | 31 +-5 | 28 +-3 | 26 +-4 | 9 +-2 | 9 +-1 | 168 +-22 | 170 +-23 | 21 +-3 | 30 +-5 | |
0.1 | 24 +-3 | 28 +-4 | 31 +-3 | 25 +-4 | 12 +-3 | 11 +-2 | 164 +-16 | 168 +-19 | 29 +-5 | 32 +-4 | |
0.5 | 14 +-7* | 38 +-5 | 18 +-2 | 22 +-3 | 10 +-3 | 10 +-2 | 169 +-18 | 173 +-16 | 35 +-5 | 32 +-4 | 0 |
1 | 0* | 10 +-4* | 0 * | 0* | 0* | 0* | 0* | 0* | 0* | 0* | |
5 | 0* | 0* | 0* | 0* | 0* | 0* | 0* | 0* | 0* | 0* | 5.5 |
DMSO | |||||||||||
0.05 ml | 26 +-5 | 28 +-6 | 22 +-4 | 20 +-5 | 8 +-3 | 10 +-2 | 181 +-26 | 191 +-29 | 30 +-5 | 34 +-4 | 0 |
ENNG | |||||||||||
2 µg | 1778 +-401 | ||||||||||
10 µg | 1389 +-331 | ||||||||||
2 -NF | |||||||||||
2 µg | 1559 +-325 | ||||||||||
5 µg | 1632 +-294 | ||||||||||
9 -AA | |||||||||||
100 µg | 1068 +-447 | ||||||||||
2 -AA | 1014 +-166 | 1322 +-238 | |||||||||
2 µg | |||||||||||
5 µg | 1082 +-351 | 185 +-41 | 161 +-29 | ||||||||
4 -NQO | |||||||||||
1 mg | 7 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under this test conditions no mutagenic effects of o-Nitrobenzaldehyde were observed. - Executive summary:
The mutagenicities of 37 mono-nitrobenzene derivatives, i.e. the ortho, meta, and para isomers of nitrotoluene, nitrophenol, nitroaniline, nitroanisole, nitrobenzaldehyde, nitrobenzyl chloride, nitrobenzonitrile, nitroacetophenone, nitrophenetol, nitrobenzoic acid, nitrophenylacetic acid, and nitrocinnamic acid and p-nitrothiophenol, were tested in the Ames test and rec assay. The rec assay gave more positive results than the Ames test. The para-substituted nitrobenzene derivatives were always positive in the rec assay as were all but 2 in the Ames test, while 5 out of 12 ortho and meta derivatives were mutagenic in the Ames test. The results of the present study, combined with those of the previous study, are discussed with special reference to the structure-mutagenicity relationships.
In this endpoint study record only the results for the substance o-Nitrobenzaldehyde (CAS 552 -89 -6) were considered.
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