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EC number: 201-178-4 | CAS number: 79-11-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Oct - 21 Oct 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Alga, growth inhibition test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Chloroacetic acid
- EC Number:
- 201-178-4
- EC Name:
- Chloroacetic acid
- Cas Number:
- 79-11-8
- Molecular formula:
- C2H3ClO2
- IUPAC Name:
- chloroacetic acid
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): Chloroacetic acid
- Physical state: non colored crystalline
- Analytical purity: 100%
_ Vapour pressure: 1 hPa at 20°C
-Water solubility: 45% at 20 °C
- Lot/batch No.: 610C2140
- Stability under test conditions: stable, verified by IR.
- Storage condition of test material: in the cool and dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.02, 0.03, 0.59, 0.10, 0.17, 0.300 mg/L
- Sampling method: Test water was sampled from all vessels and mixed in one sample for each concentration. These samples were centrifuged (3000 rpm, 10 min).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item was soluted in medium to prepare Stock solution I (1000 mg/L). Stock solution I was diluted with medium to prepare stock solution II (1 mg/L). Stock solution II was diluted with medium to make series of concentrations.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: ATCCC22662
- Source (laboratory, culture collection): American Type Culture Collection
- Age of inoculum (at test initiation): 5 days
- Method of cultivation:
ACCLIMATION
- Acclimation period: 5 days
- Culturing media and conditions (same as test or not): same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 d
Test conditions
- Test temperature:
- 22.2 - 23.0 °C
- pH:
- 8.1 - 9.8
- Nominal and measured concentrations:
- Nominal concentration: 0.02, 0.034, 0.059, 0.100, 0.170, 0.300 mg/L
Mean measured concentration: 0.0182, 0.0331, 0.0586, 0.107, 0.179, 0.316 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): closed with air permeability silicone cap
- Material, size, headspace, fill volume: glassm 300 mL,
- Initial cells density: 5 x 1000 cells/mL
- Control end cells density: 2098000 ± 206000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no adjustment
- Photoperiod: continuously
- Light intensity and quality: 75 μE/m2/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Range finding study: performed twice.
- Test concentrations:
Range finding study I
Growth inhibition after 72 hours were 2, -1, 1, 7 and -2% in 0.008, 0.009, 0.01, 0.015, and 0.02 mg/L, respectively.
Range finding study II
Growth inhibition after 72 hours were -31, -39, -30, 34, 97, 99, 99% in 0.015, 0.02, 0.03, 0.04, 0.2, 0.3, and 0.4 mg/L, respectively. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.158 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.152 - 0.164 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.066 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.05 - 0.088 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.033 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.033 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities
- Unusual cell shape: not observed.
- Colour differences: not observed.
- Aggregation of algal cells: not observed.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Test water was always colorless during the test. - Reported statistics and error estimates:
- After confirmation of equality of variance, 1-way ANOVA and Dunnet multiple comparison test was performed. In case of no statistical siginificant, the highest concentration was regarded as NOEC.
Any other information on results incl. tables
Table 1. Measured concentration of the test substance in test cultures
Nominal concentration (mg/L) |
Measured concentration (mg/L) (percent of nominal) |
Mean (a) measured concentration (mg/L) |
|||
0 hour |
24 hours |
48 hours |
72 hours |
||
Control |
< 0.0003 |
< 0.0003 |
< 0.0003 |
< 0.0003 |
- |
0.0200 |
0.0218 (109) |
0.0209 (105) |
0.0185 (93) |
0.0098 (49) |
0.0182 (91) |
0.0340 |
0.0370 (109) |
0.0373 (110) |
0.0346 (102) |
0.0195 (57) |
0.0331 (97) |
0.0590 |
0.0623 (106) |
0.0623 (106) |
0.0604 (102) |
0.0448 (76) |
0.0586 (99) |
0.100 |
0.111 (111) |
0.107 (107) |
0.109 (109) |
0.093 (99) |
0.107 (107) |
0.170 |
0.184 (108) |
0.175 (103) |
0.183 (108) |
0.172 (101) |
0.179 (105) |
0.300 |
0.328 (109) |
0.313 (104) |
0.317 (106) |
0.310 (103) |
0.316 (105) |
(a): time weighted mean
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.