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EC number: 204-552-5 | CAS number: 122-52-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Adopted according to OECD SIDS (publicly available peer reviewed source). Original document not available.
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- SIDS Initial Assessment Report For SIAM 16 (Triethyl Phosphite), 2003 Paris.
- Author:
- OECD
- Year:
- 2 003
- Bibliographic source:
- UNEP Publications
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- (adopted 1983)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Triethylphosphite
- IUPAC Name:
- Triethylphosphite
- Details on test material:
- Purity: 98.8%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- Paraffinum perliquidum
- Details on exposure:
- Dose volume: 5 mL/kg bw (10 mL/kg bw in the positive control)
- Duration of treatment / exposure:
- Not applicable.
- Frequency of treatment:
- Single treatment.
- Post exposure period:
- 16, 24, and 48 h after treatment.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1500 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophosphamide, 20 mg/kg bw, dissolved in deionised water.
Route of application: intraperitoneally. Animals were sacrificed 24 hours after the administration.
Examinations
- Tissues and cell types examined:
- Normochromatic and polychromatic erythrocytes in blood smears prepared from femur bone marrow.
- Details of tissue and slide preparation:
- PREPARATION OF SPECIMENS: at least one intact femur was prepared from each sacrificed animal, and smears were prepared according to the method as described by Schmid (Mut. Res. 31, 9-15, 1975).
- Evaluation criteria:
- EVALUATION: Coded slides were evaluated using light microscopy. Generally, 1000 polychromatic erythrocytes were counted per animal. The incidence of cells with micronuclei was determined, as well as the ratio of polychromatic to normochromatic erythrocytes (number of normochromatic erythrocytes per 1000 polychromatic ones). In addition, also the number of micronucleated normochromatic erythrocytes was determined.
ASSESSMENT CRITERIA: A result was considered positive if, at any of the intervals, there was a relevant and significant increase in the number of polychromatic erythrocytes showing micronuclei in comparison to the negative control. A test was considered negative if there was no relevant or significant increase in the rate of micronucleated polychromatic erythrocytes at any time. A test was also considered negative if there was no significant increase in that rate which according to the laboratory`s experience was within the range of negative controls.
ACCEPTANCE CRITERIA: A test was considered acceptable if the figures of negative and positive controls were within the expected range, in accordance with the laboratory`s experience and/or the available literature data. - Statistics:
- Standard deviation, Wilcoxon`s non-parametric rank sum test at a 5% significance level, or one-sided chi-square-test, if the micronulei rate for polychromatic erythrocytes was increased in the negative controls.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- Clinical signs as well as a shift in PCE/NCE ratio.
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- PRE-TEST:
In a pre-test groups of five females and five males were intraperitoneally administered with 1000 mg/kg bw, 1500 mg/kg bw, 1750 mg/kg bw and 2500 mg/kg bw triethyl phosphite dissolved in corn oil. The following symptoms were recorded up to 48 hours, starting at exposure levels of 1000 mg/kg bw: apathy, semianaesthetised state, roughened fur, staggering gait, sternal and lateral recumbency, spasm, twitching, shivering, difficulty in breathing and flat breathing. In addition, 2 of 5 animals died in the 1750 mg/kg bw group, and 4 out of 5 animals in the 2500 mg/kg bw group. As the test substance was shown to be not stable in corn oil at a concentration of 10 mg/mL, an additional study was performed with paraffinum perliquidum as vehicle. In this study no animal died after a single intraperitoneal dose of 1500 mg/kg bw. The following symptoms were recorded for up to 48 hours: apathy, roughened fur, staggering gait, sternal and lateral recumbency, spasm, shivering and difficulty in breathing.
TOXICITY:
All treated animals showed the following symptoms of toxicity after administration of 1500 mg/kg bw triethyl phosphite until sacrifice: apathy, semi-anaesthetised state, roughened fur, pallor, staggering gait, sternal position, spasm, twitching, shivering and difficulty in breathing. No symptoms were recorded for the control group. All animals survived until the end of the test.
There was an altered ratio between normochromatic and polychromatic erythrocytes with relevant variations noted for the 16 hours group.
CLASTOGENICITY:
No effect. There were also no relevant variations in results between males and females. The ratio of polychromatic to normochromatic erythrocytes was altered by the treatment with triethyl phosphite in the 16-hours group as shown in the following:
Negative control: 1000:711;
Triethyl phosphite: 1000:1450 (16 hours group);
Triethyl phosphite: 1000:940 (24 hours group);
Triethyl phosphite: 1000:1004 (48 hours group);
Positive control: 1000:680
There was no increase in the incidence of micronucleated polychromatic erythrocytes in the triethyl phosphite treated groups, whereas the positive control showed a significant increase:
Negative control: 2.0 +/- 1.3 / 1000;
Triethyl phosphite: 2.3 +/- 1.6 / 1000 (16 hours group);
Triethyl phosphite: 2.2 +/- 1.9 / 1000 (24 hours group);
Triethyl phosphite; 2.5 +/- 1.9 / 1000 (48 hours group);
Positive control: 15.9 +/- 5.9 / 1000
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
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