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EC number: 218-792-3 | CAS number: 2235-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Developmental toxicity / teratogenicity
Administrative data
- Endpoint:
- developmental toxicity
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data from secondary source
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Reregistration Eligibility Decision (RED) for test material
- Author:
- US EPA
- Year:
- 1 998
- Bibliographic source:
- Prevention, Pesticides Toxic Substances ,US EPA archive document ,September 1998 )
- Reference Type:
- secondary source
- Title:
- Reproductive and developmental toxicity study of test material
- Author:
- HSDB
- Year:
- 2 018
- Bibliographic source:
- .S. National Library of Medicine, National Institutes of Health, Health & Human Services, 2018
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Developmental toxicity study of test material was performed on female CD rats.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- N,N-diethyl-m-toluamide
- EC Number:
- 205-149-7
- EC Name:
- N,N-diethyl-m-toluamide
- Cas Number:
- 134-62-3
- Molecular formula:
- C12H17NO
- IUPAC Name:
- Diethyltoluamide
- Details on test material:
- - Name of test material (as cited in study report):DEET (N,N-Diethyl-Meta-Toluamide),- Molecular formula :C12H17NO- Molecular weight : 191.272g/mol- Substance type: Organic
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CD
- Details on test animals or test system and environmental conditions:
- No data available
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- No data available
- Analytical verification of doses or concentrations:
- not specified
- Details on mating procedure:
- mated female used
- Duration of treatment / exposure:
- 14 days (from days 6 to 15 of gestation)
- Frequency of treatment:
- daily
- Duration of test:
- No data available
Doses / concentrations
- Remarks:
- 0, 125, 250or 750 mg/kg/day
- No. of animals per sex per dose:
- Total:1000 mg/kg bw/day:25 females 125 mg/kg bw/day:25 females 250mg/kg bw/day:25 females 750mg/kg bw/day: 25 females
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- No data available
Examinations
- Maternal examinations:
- Parental animals observation and examinationsCAGE SIDE OBSERVATIONS: yes DETAILED CLINICAL OBSERVATIONS: Yes Time schedule: BODY WEIGHT: YesTime schedule for examinations: FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study): yes Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: no Compound intake calculated as time-weighted averages from the consumption and body weight gain data: Yes / No / No data: No data availableWATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): No data Time schedule for examinations: OTHER:
- Ovaries and uterine content:
- The ovaries and uterine content was examined after termination: Yes Examinations included: - Gravid uterus weight: Yes - Number of corpora lutea: No data - Number of implantations: No data - Number of early resorptions: No data - Number of late resorptions: No data - Other:
- Fetal examinations:
- - External examinations: Yes: all per litter - Soft tissue examinations: Yes: all per litter - Skeletal examinations: Yes: all per litter - Head examinations: No data
- Statistics:
- No data available
- Indices:
- No data available
- Historical control data:
- No data available
Results and discussion
Results: maternal animals
General toxicity (maternal animals)
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Clinical signs such as hypoactivity, ataxia, decreased muscle tone, foot splay, perinasal encrustation, and perioral wetness were observed; some of these signs were suggestive of neurotoxicity in this dose group because none of these signs were seen in the controls. Some of these clinical signs were seen only sporadically in the other treated groups.
- Dermal irritation (if dermal study):
- not specified
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- In the 750mg/kg /day dose group dams, there was an increase in mortality rate,
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- In the 750mg/kg /day dose group dams, there was an a reduction in body weight gain
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Description (incidence and severity):
- In the 750mg/kg /day dose group dams, there was an a reduction in food consumption,
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- not specified
- Clinical biochemistry findings:
- not specified
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not specified
- Immunological findings:
- not specified
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Description (incidence and severity):
- an increase in mean liver weights.
- Gross pathological findings:
- not specified
- Neuropathological findings:
- not specified
- Histopathological findings: non-neoplastic:
- not specified
- Histopathological findings: neoplastic:
- not specified
- Other effects:
- not specified
Maternal developmental toxicity
- Number of abortions:
- not specified
- Pre- and post-implantation loss:
- effects observed, treatment-related
- Description (incidence and severity):
- A slight increase in percent post-implantation loss was seen in the 750mg/kg/day dose group
- Total litter losses by resorption:
- not specified
- Early or late resorptions:
- not specified
- Dead fetuses:
- not specified
- Changes in pregnancy duration:
- not specified
- Description (incidence and severity):
- Migrated Data from removed field(s)
Field "Effects on pregnancy duration" (Path: ENDPOINT_STUDY_RECORD.DevelopmentalToxicityTeratogenicity.ResultsAndDiscussion.ResultsMaternalAnimals.MaternalDevelopmentalToxicity.EffectsOnPregnancyDuration): not specified - Changes in number of pregnant:
- not specified
- Other effects:
- not specified
Effect levels (maternal animals)
- Dose descriptor:
- NOAEL
- Effect level:
- > 250 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Basis for effect level:
- body weight and weight gain
- clinical signs
- food consumption and compound intake
- mortality
- organ weights and organ / body weight ratios
- pre and post implantation loss
- Remarks on result:
- other: overall no toxic effects observed
Maternal abnormalities
- Abnormalities:
- not specified
- Localisation:
- not specified
Results (fetuses)
- Fetal body weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- a statistically-significant decrease in mean fetal body weight/litter was seen in the 750mg/kg /day dose group
Migrated Data from removed field(s)
Field "Fetal/pup body weight changes" (Path: ENDPOINT_STUDY_RECORD.DevelopmentalToxicityTeratogenicity.ResultsAndDiscussion.ResultsFetuses.FetalPupBodyWeightChanges): not specified - Reduction in number of live offspring:
- not specified
- Changes in sex ratio:
- not specified
- Changes in litter size and weights:
- not specified
- Changes in postnatal survival:
- not specified
- External malformations:
- no effects observed
- Description (incidence and severity):
- The incidence of external variations and/or malformations were comparable in the control and treatment groups
- Skeletal malformations:
- no effects observed
- Description (incidence and severity):
- The incidence of skeletal variations and/or malformations were comparable in the control and treatment groups
- Visceral malformations:
- no effects observed
- Description (incidence and severity):
- The incidence of visceral variations and/or malformations were comparable in the control and treatment groups
- Other effects:
- not specified
Effect levels (fetuses)
- Dose descriptor:
- NOAEL
- Effect level:
- 250 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- not specified
- Basis for effect level:
- reduction in number of live offspring
- external malformations
- skeletal malformations
- visceral malformations
- Remarks on result:
- other: overall no developmental toxic effects observed
Fetal abnormalities
- Abnormalities:
- not specified
- Localisation:
- other: not specified
Overall developmental toxicity
- Developmental effects observed:
- not specified
- Treatment related:
- not specified
- Relation to maternal toxicity:
- not specified
- Dose response relationship:
- not specified
- Relevant for humans:
- not specified
Applicant's summary and conclusion
- Conclusions:
- No Observed Effect Level (NOEL) for developmental toxicity was considered to be 250mg/kg/day, When female CD rats were treated with test material orally.
- Executive summary:
Developmental toxicity study of test material was performed on mated female CD rats. Undiluted test material in dose concentration0, 125, 250, or 750 mg/kg/day were administered via oral gavage route from days 6 to 15 of gestation.Control animals received corn oil .Twenty-five mated female rats per group were used in study. Clinical signs, body weights, food consumption, maternal liver and gravid uterine weights, maternal ovarian and uterine examination were noted. fetal external examinations and internal soft-tissue and skeletal examinations were carried out.In the 750mg/kg/day dose dams clinical signs such as hypoactivity, ataxia, decreased muscle tone, foot splay, perinasal encrustation, and perioral wetness were observed; some of these signs were suggestive of neurotoxicity in this dose group because none of these signs were seen in the controls. Some of these clinical signs were seen only sporadically in the other treated groups. In the high-dose dams, there was an increase in mortality rate, a reduction in body weight gain and food consumption, and an increase in mean liver weights. A slight increase in percent post-implantation loss was seen in the 750mg/kg/day dose group and a statistically-significant decrease in mean fetal body weight/litter was seen in the 750mg/kg/day dose group. No additional compound-related effects were found.The incidence of external, visceral, and skeletalvariations and/or malformations were comparable in the control and treatment groups. HenceNo Observed Effect Level (NOEL) for developmental toxicity was considered to be 250mg/kg/day,When femaleCD rats were treated withtest material orally.
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