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EC number: 227-290-3 | CAS number: 5766-67-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
The inherent biodegradability of EDTN was determined by the Modified MITI Test (II) according to the OECD Guidelines for Testing of Chemicals, Method No. 302 C. Based on the result of the residue analysis, the biodegradation appeard to be 62.9%. The BOD results showed that biodegradation of EDTN was 32.6% after 14 days, 63.5% after 28 days.
Hydrolysis of ethylenediaminetetraacetonitrile(EDTN) results in the formation of ethylenediaminetetraacetate (EDTA) and ammonium. In nature hydrolysis of nitriles is catalyzed by enzymes denoted as nitrilases. Biodegradable nitriles are metabolized by microorganisms through an initial hydrolysis with nitrilases. The carboxylic acids formed by this initial reaction are utilized as carbon and energy source (Kobayashi and Shimizu, 2000; Wackett and Hershberger, 2001). Provided that EDTN is biodegradable, metabolism has to be initiated by hydrolysis. Key to justification of read-across of hydrolysis of nitriles is the unity of biochemistry first described by Kluyver and Donker (1926). The EDTA formed upon hydrolysis is not utilised by microorganisms as carbon and energy source under the conditions prescribed in the OECD 301, 302 and 303 tests (Witschel and Egli, 2001; van Ginkel et al, 1997). EDTN is therefore expected NOT to be biodegradable in OECD tests. Fortuitous biological removal of a few ammonium ions might be possible.The biodegradation found in the modified MITI (II) test (see robust summary) is, therefore, considered highly unlikely. The 64% biodegradation was calculated using an endogenous oxygen consumption of 86 mg/L and a mean oxygen consumption in the presence of the test substance of only 110 mg/L. The slight increase in oxygen consumption does not allow an accurate assessment of the biodegradability with the BOD. The nitrification potential in the MITI (II) test due to the biomass concentration used is high. Moreover, it is also expected that biodegradation starts by liberating ammonium which immediately results nitrification. Use of theThODNO3instead of ThODNH3to calculate the biodegradation is therefore more appropriate. With the ThODNO3the biodegradation percentage at day 28 is only 27. An almost complete recovery of EDTN is achieved using extraction with acetonitrile in the abiotic control. In the test, however, high concentrations of activated sludge are present. The 63% degradation found with the residue analysis is therefore most likely the result of extraction problems. In conclusion classification of EDTN as inherent biodegradable with the results obtained in the MITI (II) test is very doubtful.
The lack of EDTN biodegradation in OECD tests does not mean that this substance is recalcitrant in nature because the stringency of the test procedures could account for the recalcitrance. The OECD tests do only detect growth-linked biodegradation. Degradation of EDTN (removal of ammonium through hydrolysis by a co-metabolic (fortuitous) process) is envisaged.
The analogue substance PDTN was also not readily biodegradable (see also at section 13). However, both PDTN and EDTN are used as intermediates with no release to the environment.
References
Ginkel C.G van, K.L. VandenBroucke and C.A. Stroo (1997) Bioresour. Technol. 59: 151 -155
Key Lab of pesticide Environmental Assessment and Pollution Control MEP (2009) Report for inherent biodegradation Modified MITI (II) test EDTN Report no R2009NC007-02(see robust summary)
Kluyver A.J. and H.J.L. Donker (1926) Chemie der Zelle und Gewebe, 13: 134‑190Kobayashi M. and S. Shimizu (2000) Nitrile hydrolases. Current Opinion in Chemical Biology 4: 95 -102
Wackett P. and C.D. Hershberger (2001) Biocatalysis and Biodegradation Microbial transformation of organic compounds. ASM Press, Washington DC 20036 -2904
Witschel M. and T. Egli (2001) FEMS Microbiol. Rev. 25: 69-106
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