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EC number: 609-904-5 | CAS number: 41263-74-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The experimental design of the study consists of a test for Direct Reduction of MTT by the test material, followed by the main test.
For the main test, triplicate SkinEthic tissues were treated with 30 mg oftest material and exposed for 10 minutes and 60 minutes. The tissues were
incubated at 37°C in a humidified atmosphere of 5% C02 in air for the appropriate exposure period.
Duplicate tissues served as the negative control or positive control for each exposure period.
At the end of the 10 minute exposure period each SkinEthic tissue was rinsed using Dulbecco' s Phosphate Buffered Saline (DPBS) and placed into
a 'holding plate' until all the tissues had been rinsed. The rinsed tissues (two per group) were then transferred to an MTT 'loading plate', and
incubated at 37°C for approximately 3 hours in a humidified atmosphere of 5% C02 in air. The remaining test material treated tissue was retained for possible histology. Following MTT loading, each SkinEthic tissue was blotted dry and placed into an MTT 'extraction plate' in order to extract aIl of the reduced MTT from the tissues. The same rinsing, retention, loading and extraction procedures were repeated for the 60 minute tissues once
the 60 minute exposure period was complete.
At the end of the extraction period, the extracted MTT solution was mixed for each SkinEthic tissue and 3 x 200 111 sampies, representing each
tissue, were transferred to the appropriate weIls of a 96 weIl plate. The optical density at 540nm (ODS40) of each weIl was measured.
Data arepresented in the form of % viability (MTT conversion relative to negative controls) for each of the two exposure periods.
The results were used to make a prediction of the eye irritation potential of the test material. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4-(methylamino)-3-nitrobenzoic acid
- EC Number:
- 609-904-5
- Cas Number:
- 41263-74-5
- Molecular formula:
- C8 H8 N2 O4
- IUPAC Name:
- 4-(methylamino)-3-nitrobenzoic acid
Constituent 1
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- other: relative mean viability [%]
- Value:
- 92.7
- Remarks on result:
- other: exposure time: 10 minutes
- Irritation parameter:
- other: relative mean viability [%]
- Value:
- 92
- Remarks on result:
- other: exposure time: 60 minutes
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Negative result, not likely to be a severe ocular irritant.
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