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EC number: 939-512-2 | CAS number: 85681-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-05-19 to 2003-12-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:1.0, 2.2, 4.6, 10, 22, 46 and 100 mg/L and blank control
- Sampling method: 0 h, 24 h and 72 h
10 mL from approximate centre of vessels
- Sample storage conditions before analysis: Not applicable, samples analysed on day of sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test solutions with a concentration of 1.0. 10 and 100 mg/L were prepared seperately. After a 10 minutes treatment with ultrasnic waves to accelerate the disolving of the test substance in the test medium, the test solutions were clear and colourless but the 10 and 100 mg/L concentrations contained a layer of foam. After the foam had disappeared from the highest test concentrations a ten-fold dilution was prepared from the 1.0 mg/L concentration. The final test solutions were clear and colourless
Final: Test concentrations prepared from 100 mg/L stock solution. 10 minutes magnetic stirring used to accelerate disolving of the test substance in the test media. Layer of foam dissolved in test medium by use of air pressure.
- Controls: Blank
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Thin foam on test concentrations in final test. - Test organisms (species):
- Scenedesmus capricornutum
- Details on test organisms:
- TEST ORGANISM
- Common name: Blue/green algae
- Strain: NIVA CHL 1
- Source: In-house laboratory culture with known history
- Age at study initiation (mean and range, SD):
- Method of breeding: Inncoculation of growth medium with algal cells from a pure culture on agar. Suspensions continuously aerated and exposed to light at 23 ± 2°C
- Feeding during test: Not applicable
ACCLIMATION
- Acclimation period: 3 days (pre-culture)
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Medium M2 (ISO-standard)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not applicable, continuous culture - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Hardness:
- 0.24 mmol (24 mg/L CaCO3)
- Test temperature:
- 23 ± 2°C
- pH:
- 8.3 ± 0.2
- Dissolved oxygen:
- Not measured
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 1.0, 2.2, 4.6, 10, 22, 46 and 100 mg/L (nominal)
Average exposure concentrations: 0.36, 0.86, 1.7, 3.4, 7.2, 14 and 33 mg/L (see attached document 03-006F Exposure concentrations)
Analysis of test samples in the final test showed the test cncentrations were not stable especially over the last 48 h of the test period. The possible cause of loss of the test mateial was biodegradation. During the exposure period test solutions became gradually turbid probabaly due to bacterial growth.
The average exposure concentrations based on peak 1 and 2 were calculated and proved to be quite similar. Based on better analytical recoveries for peak 2, toxicological effects were related to the average expsure concentrations calculated for peak 2. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass
- Material, size, headspace, fill volume: 50 mL test solution + 1 mL algal suspension
- Aeration: Not specified
- Renewal rate of test solution (frequency/flow rate): Not renewed
- Initial cells density: 1E04 cells/mL
- Control end cells density: 1E04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
Also 1 replicate of each test concentration without algae and 1 extra replicate of each test concentration and the blank-cintrol for sampling purposes.
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-Q water
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: No
- Photoperiod: constant
- Light intensity and quality: TLD Lamps of the type cool-white of 30 watt with light intensity 73 - 96 µE/m2/sec
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope and a counting chamber and spectrophotometer
- Chlorophyll measurement: No
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.1 -100 mg/L increasing by a factor of 10
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate - periodical check on sensitivity of test system
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL = 0.69 - 5.2 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.45 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL = 0.15 - 1.29 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.36 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL = 3.2 - 14 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL = 0.68 - 3.1 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.86 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The test material reduced growth rates of Selenastrum capricornutum significantly at average exposure concentrations of 0.36 mg/L. upwards resulting in 99% inhibition at an average exposure concentration of 31 mg/L. Statistically signficant inhibition of cell growth was found at all average exposure concentrations tested (Bonferroni t and Tukey test).
Growth rates were in the range of the controls at the average exposure concentrations of 0.36 and 0.86 mg/L duringthe 72 h test period, whereas the growth rate of algae exposed to 1.7 mg/L and higher were increasingly reduced.
Although growth rate was reduced it increased as exposure progressed at average test substance concentrations of 1.7 up to 7.2 mg?l. Only the highest average test concentration (33 mg/L) growth rate decreased as exposure progressed. Statistically significant reduction of growth rate was found at average test concentrations of 1.7 mg/L and higher.
During the exposure period test solutions became gradually turbid, porbably due to baterial growth. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- ErC50: 0.89 mg/L (95% CL = 0.56 - 1.4 mg/L)
- Other: ISO standard ErC50 = 0.60 - 1.03 mg/L - Reported statistics and error estimates:
- Analysis of test samples in the final test showed the test cncentrations were not stable especially over the last 48 h of the test period. The possible cause of loss of the test mateial was biodegradation. During the exposure period test solutions became gradually turbid probabaly due to bacterial growth.
The average exposure concentrations based on peak 1 and 2 were calculated and proved to be quite similar. Based on better analytical recoveries for peak 2, toxicological effects were related to the average expsure concentrations calculated for peak 2. - Validity criteria fulfilled:
- yes
- Conclusions:
- The test material reduced growth rates of Selenastrum capricornutum significantly at average exposure concentrations of 0.36 mg/L. upwards resulting in 99% inhibition at an average exposure concentration of 31 mg/L.
The ErC50 was 6.8 mg/L (95% confidence limits: 3.2 - 14 mg/L), the ErC10 was 1.5 mg/L (95% confidence limits: 0.68 - 3.1 mg/L) and the NOEC (growth rate) was 0.86 mg/L.
During the exposure period test solutions became gradually turbid, porbably due to baterial growth. - Executive summary:
In a 72 -hour algal inhibition study performed in accordance with OECD No 201, EU Method C3 and ISO Standard 8692 the test material was dosed at nominal concentrations of 1.0, 2.2, 4.6, 10, 22, 46 and 100 mg/L to Selenastrum capricornutum. The dose concentrations were chosen using a range finding study. A blank control was run in conjunction with the final test. Results of a positive control study on potassium dichromate were appended to the study to confirm sensitivity of the test system.
Analysis of the samples showed that the test material was unstable in the test medium after 48 hours and had decreased to below detection level after 72 hours. The Study Director indicated that loss was probably due to biodegradation of the test material. The average exposure concentrations were therefore 0.36, 0.86, 1.7, 3.4, 7.2, 14 and 33 mg/L.
The study met the acceptability criteria and was considered valid.
During the exposure period test solutions became gradually turbid probabaly due to bacterial growth.
The test material reduced growth rates of Selenastrum capricornutum significantly at average exposure concentrations of 0.36 mg/L. upwards resulting in 99% inhibition at an average exposure concentration of 31 mg/L.
The ErC50 was 6.8 mg/L (95% confidence limits: 3.2 - 14 mg/L), the ErC10 was 1.5 mg/L (95% confidence limits: 0.68 - 3.1 mg/L) and the NOEC (growth rate) was 0.86 mg/L.
Reference
Table 1: % inhibition of cell growth during the final test
Average test material conc. (mg/L) |
Cell growth (0-72 h) |
|
Mean area (A) |
Inhibition (%) |
|
Blank control |
1745.04 |
|
0.36 |
1460.92 |
16.3 |
0.86 |
1440.48 |
17.5 |
1.7 |
1104.92 |
36.7 |
3.4 |
571.64 |
67.2 |
7.2 |
89.60 |
94.9 |
14 |
114.04 |
93.5 |
33 |
15.88 |
99.1 |
Table 2: % reduction of growth rate at different time intervals
Average test material conc. (mg/L) |
Mean growth rate |
|||||
µ (0-24 h) |
Reduction (%) |
µ (0-48 h) |
Reduction (%) |
µ (0-72 h) |
Reduction (%) |
|
Blank control |
0.04763 |
|
0.06644 |
|
0.06332 |
|
0.36 |
0.04356 |
8.6 |
0.06270 |
5.6 |
0.06079 |
4.0 |
0.86 |
0.03973 |
16.6 |
0.06172 |
7.1 |
0.06100 |
3.7 |
1.7 |
0.03156 |
33.7 |
0.05636 |
15.2 |
0.05750 |
9.2 |
3.4 |
0.01420 |
70.2 |
0.04272 |
35.7 |
0.04800 |
24.2 |
7.2 |
0.01134 |
76.2 |
0.01273 |
80.8 |
0.02486 |
60.7 |
14 |
0.03175 |
33.3 |
0.01373 |
79.3 |
0.02506 |
60.4 |
33 |
0.00944 |
80.2 |
0.00437 |
93.4 |
0.00259 |
95.9 |
Description of key information
Selenastrum capricornutum were exposed to the substance under static conditions for 72 hours in a GLP study performed according to OECD TG 201. The ErC50 (0 -72h) was 6.8 mg/L and the NOEC was 0.86 mg/L, both based on mean measured concentrations.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 6.8 mg/L
- EC10 or NOEC for freshwater algae:
- 0.86 mg/L
Additional information
One reliable study is available. In this study performed under GLP according to OECD TG 201 Selenastrum capricornutum (3 replicates/concentration) were exposed under static conditions to the substance for 72 hours at nominal concentrations of 10, 2.2, 4.6, 10, 22, 46 and 100 mg/L [Bouwman LM (2003b)]. The ErC50 (0 -72h) was 6.8 mg/L and the NOEC was 0.86 mg/L, both based on mean measured concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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