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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed guideline study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
Qualifier:
equivalent or similar to guideline
Guideline:
other: draft ISO/DIS 10712 (1994)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3-nitro-p-toluic acid
EC Number:
202-549-3
EC Name:
3-nitro-p-toluic acid
Cas Number:
96-98-0
Molecular formula:
C8H7NO4
IUPAC Name:
4-methyl-3-nitrobenzoic acid
Details on test material:
purity: 99.4 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture:
- Method of cultivation: analogous DIN 38 412 Teil 8 (1991)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
16 h

Test conditions

Test temperature:
21+-1 °C
pH:
7.3
Nominal and measured concentrations:
nominal: 500 mg/L and 1000 mg/L
Reference substance (positive control):
yes
Remarks:
2,4-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
other: 0.3 % inhibition
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
other: 19.6 % inhibition
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Results with reference substance (positive control):
2,4-Dichlorophenol after 16 h
30 mg/L : 26.2 % inhibition
90 mg/L: 97.6 % inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The substance is not toxic to Pseudomonas putida
Executive summary:

The substance was tested for toxicity for Pseudomonas putida according to DIN 38 412 Teil 8 (1991) by photometric determination of the cell density. 2,4 -Dichlorophenol was used as positive reference substance. After 16 hours the inhibition was 19.6 % with 1000 mg/L and 0.3 % with 500 mg/L. The EC 50 is greater than 1000 mg/L