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EC number: 427-720-1 | CAS number: 26364-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study according OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 427-720-1
- EC Name:
- -
- Cas Number:
- 26364-65-8
- Molecular formula:
- C4H5N3S
- IUPAC Name:
- [(1,3-thiazolidin-2-ylidene)amino]formonitrile
- Details on test material:
- name: Cyanamide, (4,5-dihydroxy-2-thiazolyl)-
molecular formula: C4 H5 N3 S
molecular weight: 127.2
physical state: solid
analytical purity: 98 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 178 mg/l CaCO3
- Test temperature:
- 20.2°C
- pH:
- at the start of the study: 7.9
at the end of the study: 8.0 - Dissolved oxygen:
- at the start of the study: 9.4
at the end of the study: 8.7 - Nominal and measured concentrations:
- see below
- Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % CL-limits: 29-43 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- An acute toxicity test was carried out on September 24, 1996 under the same conditions using the
reference substance K2Cr2O7, reagent grade (test concentrations: 0.75, 1.00, 1.33, 1.78, 2.37
and 3.16 mg/liter). The 24 hour EC50 of 1.71 mg/1 (95% confidence limits 1.58 - 1.85 mg/l)
determined in this test lies within the required range of 0.9 - 1.9 mg/l. The gradient of the line of
regression (after Litchfield & Wilcoxon) was s = 1.37. ('Results on the toxicity to water fleas of
the reference substance potassium dichromate', report HBF/Dm 163 of September 25, 1996).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48 hours) was 35 mg/l.
The substance is subject to classification based on the results of this study. - Executive summary:
The control mortalities are below the 10 % value which is regarded as the limit for natural
mortality. As the physico-chemical measurements show, the composition of the test water
corresponds to the nominal values. The EC50 of the reference substance lies within the required
range. Thus, the study conditions correspond to the standard.
At the beginning of the test, the content of active ingredient was analyzed at all test
concentrations. The measured concentrations were 103.9 % to 108.0 % (mean: 106.8 %) of the
nominal ones. A stability test at the end of the 48 hours exposure period showed no degradation
of the test substance. These results indicate that the test concentrations prepared in this test
correspond to nominal concentrations for the study duration.
The EC50 (24 hours) determined for 2-Cyanimino-l,3-thiazolidine was about 100 mg pure
substance/l and the EC50 (48 hours) was 35 mg pure substance/1 (95 %-confidence limits 29 to 43
mg pure substance/l).
Based on nominal concentrations, the no-observed-effect-concentration (NOEC) after 24 hours
was 18 mg pure substance/l and after 48 hours 10 mg pure substance/l, the lowest-observedeffect-
concentration (LOEC) after 24 hours was 32 mg pure substance/l and after 48 hours 18 mg
pure substance/l. The threshold effect concentration after 24 hours was 24 mg pure substance/l
and after 48 hours 13 mg pure substance/l (TEC, geometric mean of NOEC and LOEC).
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