Indeno[4,3a-b]furan, decahydro-2,2,7,7,8,9,9-heptamethyl-

Long-term toxicity to aquatic invertebrates

Description of key information

An OECD TG 211 study in compliance with GLP criteria on Daphnia magna is available for the substance. The test organism (1/replicate; 10 replicate/concentration) were exposed to nominal concentrations of 0 (negative control; dilution water), 0 (solvent control; 0.005% DMF), 7.5, 15, 30, 60 and 120 µg/L under semi-static conditions (media renewed daily) at 20˚C for 21 days. The test concentrations were analytically verified and determined as < Method Detection Limit (MDL), < MDL, 3.9, 7.3, 15.5, 34.1 and 78.6 µg/L, respectively (time-weighted mean measured concentrations; 49 - 65% nominal). Test conditions were maintained constant throughout the study. The dissolved oxygen concentration has been at least 60% of the air saturation value throughout the test. After 21 days exposure, adult survival was 90% to 100% among the treatments, except for the highest test concentration (100% mortality on day 4). The mean number of produced neonates per surviving parent animal ranged from 107 to 137. All produced neonates were normal. No aborted eggs or other unusual behaviour were observed. There were no significant effects of treatment on reproductive output or growth of surviving parent animals. The most sensitive parameter was survival of the adults (100% at 78.6 ug/l. Absence of reproductive effects at the next concentration resulted in a NOEC of 34.1 µg/L, based on time-weighted mean measured concentration.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

34.1 µg/L