Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro, other
- Remarks:
- mammalian cell gene mutation assay; sister chromatid exchange assay in mammalian cells; DNA damage and repair assay in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The genotoxicity of lead acetate was investigated in the presence or absence of UV irradiation. A DNA strand break assay in HeLa cells was used to examine DNA damage associated with exposure to 500 uM lead acetate. In V79 cells, 0.5 to 5 uM lead acetate was used in a HPRT locus mutation assay and 1 to 10 uM lead acetate was used in a sister-chromatid exchange (SCE) assay.
- GLP compliance:
- not specified
- Type of assay:
- other: mammalian cell gene mutation assay; sister chromatid exchange assay in mammalian cells; DNA damage and repair assay in mammalian cells
Test material
- Reference substance name:
- Acetic acid, lead salt, basic
- EC Number:
- 257-175-3
- EC Name:
- Acetic acid, lead salt, basic
- Cas Number:
- 51404-69-4
Constituent 1
Results and discussion
Test results
- Species / strain:
- other: Human HeLa cells; Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Remarks on result:
- other: Test system: all strains/cell types tested
Any other information on results incl. tables
Lead acetate alone did not induce DNA strand breaks, but UV-induced strand breaks persisted longer in the presence of lead acetate compared to UV-irradiation alone. Lead acetate alone did not induce mutations at the HPRT locus, but did enhance UV-induced mutagenicity between 0.5 and 5 uM. Lead acetate alone did not induce an increase in SCEs, but did enhance the number of UV-induced SCEs at all concentrations tested.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
- Executive summary:
The genotoxicity of lead acetate was investigated in the presence or absence of UV irradiation. A DNA strand break assay in HeLa cells was used to examine DNA damage associated with exposure to 500 uM lead acetate. In V79 cells, 0.5 to 5 uM lead acetate was used in a HPRT locus mutation assay and 1 to 10 uM lead acetate was used in a sister-chromatid exchange (SCE) assay. Lead acetate alone did not induce DNA strand breaks, but UV-induced strand breaks persisted longer in the presence of lead acetate compared to UV-irradiation alone. Lead acetate alone did not induce mutations at the HPRT locus, but did enhance UV-induced mutagenicity between 0.5 and 5 uM. Lead acetate alone did not induce an increase in SCEs, but did enhance the number of UV-induced SCEs at all concentrations tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.