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EC number: 475-290-9 | CAS number: 39537-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-07-21 to 1994-07-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted 4 April, 1984
- Deviations:
- yes
- Remarks:
- Test concentrations exceeded recommended highest concentration of 1 g/L
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations, freshly prepared and aged test solution after 0, 24 and 48 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 2.5 g test substance to 250 mL solution water. The test concentrations were prepared separately by diluting the stock solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): 6 - 24 h
- Source: bred at testing facility originally obtained from LWA Düsseldorf, Germany
- Feeding during test: no - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.0 - 21.0 °C
- pH:
- 6.17 - 7.86
- Dissolved oxygen:
- 6.5 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations: control, 4096, 8192 and 10000 mg/L
The recovery rate was > 90%.
Measured at 0 h: 3953, 7941 and 9692 mg/L (new media)
Measured at 24 h: 3913, 8461 and 9830 mg/L (old media); 4179, 8373 and 9990 (new media)
Measured at 48 h: 4077, 8291 and 10226 mg/L (old media) - Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): covered with paper
- Volume of solution: 10 mL
- Renewal rate of test solution: after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water (ISO test water)
- Intervals of water quality measurement: at test start and after 24 h and 48 h
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night cycle
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation after 0, 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 1, 10, 100, 1000 and 10000 mg/L
- Results used to determine the conditions for the definitive study: no immobilised Daphnia after 48 h - Reference substance (positive control):
- yes
- Remarks:
- potassium-dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 8 192 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no
- Results with reference substance (positive control):
- Tested once per month, results of the latest testing: EC50 (48 h): 1.4 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test procedure a acute toxic effect of the test substance towards daphnia was found after 48 hours in the test solution of 10 g/L resulting in an EC50 (48 h) of 10000 mg/L.
Reference
Description of key information
EC50 (48 h): 10000 mg/L (nominal)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10 000 mg/L
Additional information
One study investigating the acute toxicity of the substance to aquatic invertebrates is available. The study was conducted according to OECD guideline 202, part I (1984, GLP). Daphnia magna were exposed to nominal test substance concentrations of 4096, 8192 and 10000 mg/L incl. a control. The test solutions were renewed after 24 hours and the test concentration was analytically verified by HPLC. The recovery rates in the freshly prepared and in the aged test solutions were >90%. Thus, the effect concentrations were based on nominal concentrations. An immobilisation of 50% was observed at the highest test concentration of 10000 mg/L after 48 hours. Based on this result an EC50 (48 h) of 10000 mg/L was determined.
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