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EC number: 220-115-1 | CAS number: 2631-68-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Remarks:
- DNTCB (1,3,5-trichloro-2,6-dinitrobenzene) EVALUATION OF ACUTE ORAL TOXICITY IN RATS - Acute toxic class method
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- yes
- Remarks:
- Environmental parameters: A temperature higher than 25°C was registered and A relative humidity lower than 30%. As no effect was noted on the health of the animals, this deviation is considered as without impact on the conclusion of the study.
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- 1,3,5-trichloro-2,6-dinitrobenzene
- Cas Number:
- 6284-83-9
- IUPAC Name:
- 1,3,5-trichloro-2,6-dinitrobenzene
- Reference substance name:
- 1,3,5-trichloro-2,4-dinitrobenzene
- EC Number:
- 228-510-0
- EC Name:
- 1,3,5-trichloro-2,4-dinitrobenzene
- Cas Number:
- 6284-83-9
- Molecular formula:
- C6HCl3N2O4
- IUPAC Name:
- 1,3,5-trichloro-2,4-dinitrobenzene
- Test material form:
- solid: particulate/powder
- Details on test material:
- • Date received: 17 September 2021
• CAS No.: 6284-83-9
• EINECS No.: 228-510-0
• Container: plastic flask (n=1)
• Category: Chemical Substance
• Quantity: 111.70 g (container + content + label)
• Storage: room temperature
• Homogeneity: yes
• Form: solid (powder)
• Stability: not specified
• Colour: yellowish
• Production date: not specified
• Expiry date: February 2024
• Composition: 1,3,5-trichloro-2,6-dinitrobenzene • Purity: 99.24%
It was identified under the code number: PH-21/0912.
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- The test item DNTCB (1,3,5-trichloro-2,6-dinitrobenzene) was administered to a group of 6 female
Sprague Dawley rats at the dose of 300 mg/kg body weight, then to a group of 6 female Sprague
Dawley rats at the dose of 2000 mg/kg body weight. The experimental protocol was established
according to the official method as defined in the O.E.C.D. Test Guideline No. 423 dated December
17th, 2001.
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- olive oil
- Details on oral exposure:
- Drinking water (tap-water from public distribution system) and foodstuff (ENVIGO – 2014 and 2016)
were supplied ad libitum. Food was removed on day 1 and then redistributed 4 hours after the test item administration. - Doses:
- 300 and 2000 mg/kg
- No. of animals per sex per dose:
- Group treated (300 mg/kg): 3 female rats Rf6292 to Rf6294 (Step 1); 3 female rats Rf6298 to Rf6300 (Step 2)
Group treated (2000 mg/kg): 3 female rats Rf6335 to Rf6337 (Step 3); 3 female rats Rf6338 to Rf6340 (Step 4)
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Group treated at 300 mg/kg:
No mortality was noted in animals treated at the dose of 300 mg/kg body weight.
Group treated at 2000 mg/kg:
Two mortalities were noted in animals treated at the dose of 2000 mg/kg body weight at 3 hours post-treatment. - Clinical signs:
- bodyweight loss
- convulsions
- other: dyspnea/decrease of spontaneous activity
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 of the test item DNTCB (1,3,5-trichloro-2,6-dinitrobenzene) is higher than 2000 mg/kg
body weight.
In accordance with the O.E.C.D. Test Guideline No. 423, the LD50 cut-off of the test item may be
considered as 2500 mg/kg body weight by oral route in the rat.
The test item DNTCB (1,3,5-trichloro-2,6-dinitrobenzene) does not have to be classified in
accordance with the Regulation EC No. 1272/2008 on classification, labelling and packaging of
substances and mixtures.
No signal word or hazard statement is required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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