Hexane-1,3,6-tricarbonitrile

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019.11.08-2020.1.10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout

- Source:Trout Lodge Trout Farm (Sumner, WA)
- Age at study initiation (mean and range, SD):Junvenile, 40days
- Length at study initiation (length definition, mean, range and SD):34±4mm
- Weight at study initiation (mean and range, SD):0.4263±0.12g

ACCLIMATION
- Acclimation period:12days
- Acclimation conditions (same as test or not):same
- Type and amount of food during acclimation:not specified
- Feeding frequency during acclimation:daily
- Health during acclimation (any mortality observed): NA

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

80 mg/L CaCO3

Test temperature:

11℃

pH:

7.7

Dissolved oxygen:

100 mg/L

Conductivity:

3.13.9-3.15-8 μmhos/cm

Nominal and measured concentrations:

1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:EC chamber C
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 2 gallon glass aquaria
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- Renewal rate of test solution (frequency/flow rate):no
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):3
- No. of vessels per vehicle control (replicates):10
- Biomass loading rate:1 g fish/L



TEST CONCENTRATIONS
- Range finding study: 0.1, 1, 10, 50, and 100 mg/L
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: limit test concentration from OECD203

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The NOEC of Tricyanohexane was determined to be 100mg/L, and the LC50 was determined to be >100mg/L.

Executive summary:

This study was conducted to assess the toxicity of the test substance,Tricyanohexane, to rainbow trout,Oncorhynchus mykiss,in a 96-hour static test.

Test concentrations were determined by preliminary range-finding test. The range-finder indicated aLC5o (Median Lethal Concentration) of>100 mg/L, so a limit test was conducted in lieu of a definitive.The limit test was conducted using the test substance at a concentration of 100 mg/L and was administered to the fest system, Oncorhynchus mykiss, in moderately hard synthetic freshwater. Three replicates of ten organisms each were treated. A control group containing three control replicates often organisms was not exposed to the test substance.Dissolved oxygen, temperature, conductivity and pH measurements were recorded at dosing and termination.Observations for survival in each test chamber were made daily.The test was terminated after 96± 1 hours of exposure. A mortality rate of 0.00% was observed in the control and organisms treated with 100 mgL. Therewere no observable abnormalities in either group.The No Observed Effect Concentration (NOEC) of Tricyanohexane was determined to be 100 mg/L, and the Median Lethal Concentration (LC50) was determined to be > 100mg/L.Results presented are based on nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L