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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003/07/21 to 2003/09/04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Study performed under GLP conditions. The guidelines followed in this study are OECD guideline 202 part 1 91884, JMAFF (2-7-2-1) (2001) and EU Commission Directive 92/69/EEC C.2 (1992). No deviations occurred from these guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part 1 91884
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF
- Version / remarks:
- 2-7-2-1, 2001
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- The test material is Cyromazine, it is a white, light beige solid crystalline powder.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Five Daphnia magna (6-24 hours old and not first progeny brood) were added to each of four replicate vessels for each treatment and control. The daphnids were not fed for the duration of the test. The Daphnia magna were assessed visually for signs of immobility after 24 and 48 hours. All samples were deep-frozen (at about -20 °C) immediately after.
Test solutions
- Vehicle:
- yes
- Remarks:
- reconstituted water
- Details on test solutions:
- The test medium of the highest nominal concentration of 100 mg/L was prepared by dissolving 100 mg of test item completely in 1000 mL of test water using ultrasonic treatment for 15 minutes and intense stirring for 15 minutes at room temperature. In a series of different dilution steps this test medium was diluted with test water to prepare the test media with the lower test item concentrations as stated above. The test media were prepared just before introduction of the Daphnia magna (= start of the test).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Age at study initiation: 6-24 hours old and not first progeny brood
- Diet: Not fed during test
Study design
- Test type:
- static
- Water media type:
- other: reconstituted water
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/L as CaCO3
- Test temperature:
- water was at constant 20°C
- pH:
- 7.6 to 7.7
- Dissolved oxygen:
- 8.5 mg/L
- Nominal and measured concentrations:
- Nominal Concentrations: 1.0, 2.2, 4.6, 10, 22, 46, 100 mg/L
- Details on test conditions:
- Test vessel consisted of a 100mL glass beaker (with a lid) containing 50 mL of the appropriate test solution. The water was aerated until oxygen saturation prior to initiation but for the 48-hours duration of the test the vessels were not aerated. Analytical determination of the test substance concentrations were made at test start and end.
- Reference substance (positive control):
- yes
- Remarks:
- Control in this experiment was untreated reconstituted water
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- The measured concentrations at test start or at test end were 99% to 108% of nominal All results are therefore presented in terms of nominal concentration
Immobilisation and symptoms of toxicity
Any other information on results incl. tables
Physical parameters monitored:
The water was at a constant 20°C and measured light intensity ranged from 460-670 lux (16-hour photoperiod). The pH of the test medium and the control ranged from 7.6 to 7.7 and the water hardness was 2.5 mmol/L as CaCO3. Dissolved oxygen concentration in the test media was at least 8.5 mg/L.
Analytical findings:
The measured concentrations at test start or at test end were 99% to 108% of nominal All results are therefore presented in terms of nominal concentration
Immobilisation and symptoms of toxicity:
Table 1
Acute toxicity of test material on the mobility of Daphnia magna in a 48-hour static study
Nominal concentration (mg/L) | Immobilised Daphnia magna after 24 hours | Immobilised Daphnia magna after 48 hours | ||
No. | % | No. | % | |
Control | 0 | 0 | 0 | 0 |
1.0 | 0 | 0 | 0 | 0 |
2.2 | 0 | 0 | 0 | 0 |
4.6 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | 4 | 20 |
22 | 0 | 0 | 5 | 25 |
46 | 0 | 0 | 7 | 35 |
100 | 0 | 0 | 7 | 35 |
The 48-h NOEC was determined to be 46 mg cyromazine/L and the 48-h LOEC was 10 mg cyromazine/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 of cyromazine with Daphnia magna was >100 mg/L.
- Executive summary:
The acute toxicity of the test item cyromazine to Daphnia magna was determined in a 48-hour static test according to the JMAFF Test Guideline, 2-7-2-1 (2001), the EU Commission Directive 92/69/EEC, Part (1992), and the OECD Guideline for Testing of Chemicals, No. 202, Part I (1984). This study followed the procedures indicated by the following internationally accepted guidelines and recommendations.
The nominal test item concentrations tested were 1.0, 2.2, 4.6, 10, 22, 46, and 100 mg/L. Additionally, a control was tested in parallel. During the test period the test item concentrations in the test media were measured in the range from 99 to 108 % of the nominal values. Under the conditions of the test the test item
Cyromazine was stable in the test media during the test period of 48 hours. The 24-hour EC50 was > 100 mg/L along with the 24 hour EC100. The 48 hour EC50 was > 100 mg/L Therefore, all reported results are related to the nominal concentrations of the test item.
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